2012
DOI: 10.1371/journal.pone.0042776
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Effective Elicitation of Human Effector CD8+ T Cells in HLA-B*51:01 Transgenic Humanized Mice after Infection with HIV-1

Abstract: Humanized mice are expected to be useful as small animal models for in vivo studies on the pathogenesis of infectious diseases. However, it is well known that human CD8+ T cells cannot differentiate into effector cells in immunodeficient mice transplanted with only human CD34+ hematopoietic stem cells (HSCs), because human T cells are not educated by HLA in the mouse thymus. We here established HLA-B*51:01 transgenic humanized mice by transplanting human CD34+ HSCs into HLA-B*51:01 transgenic NOD/SCID/Jak3−/− … Show more

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Cited by 14 publications
(17 citation statements)
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“…In the latter case, the source of human CD34 + cells was from a type 1 diabetic patient [27]. HIV-1-specific CD8 + T cells restricted by HLA-B51 [64] and HLA-B57 [19] were also shown to be induced in HIV-1-infected B51-Tg NSG mice and BLT mice, respectively.…”
Section: Discussionmentioning
confidence: 98%
“…In the latter case, the source of human CD34 + cells was from a type 1 diabetic patient [27]. HIV-1-specific CD8 + T cells restricted by HLA-B51 [64] and HLA-B57 [19] were also shown to be induced in HIV-1-infected B51-Tg NSG mice and BLT mice, respectively.…”
Section: Discussionmentioning
confidence: 98%
“…Two additional HLA class I transgenes have been tested in humanized mice, namely a fully human HLA-A2.1 molecule (termed Enge) in NSG mice91015 and a fully human HLA-B51.1 molecule in NOK mice11 (Table 1). The NOK mice are NOD.…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, to make the experiment easier and more effective as previously suggested [9], we constructed humanized NOJ mice by transplanting CD34 + HSCs isolated from umbilical cord blood into the liver of newborn mice. The present study used hNOJ mice that were not irradiated prior to HSC transplantation (i.e., hNOJ (IR−) mice), in comparison with irradiation-treated hNOJ mice (i.e., hNOJ (IR+) mice) that have been already established [18], [47], [48]. Each hematopoietic cell population, such as T cells, B cells, monocytes, and dendritic cells in irradiation-treated hNOJ mice show the same developmental characteristics [18], [47], [48] as those in other conventional humanized mouse models constructed with different strains using the same method (newborn mice were irradiated prior to intrahepatic transplantation of umbilical cord blood-derived HSCs) [16], [17], [49].…”
Section: Discussionmentioning
confidence: 99%