Effective protection by high efficiency bicistronic DNA vaccine against infectious bursal disease virus expressing VP2 protein and chicken IL-2

Kumar, Sachin; Ahi, Yadvinder S.; Salunkhe, Shardul S.; Koul, Monika; Tiwari, Ashok Kumar; Gupta, Praveen Kumar; A, Rai

doi:10.1016/j.vaccine.2008.11.085

Cited by 34 publications

(20 citation statements)

References 27 publications

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“…Moreover, it has been reported that IFN-γ and IL-2 can be used as adjuvants to enhance antigen presentation, T cell immune response and B cell humoral immune response (Lowenthal et al, 1998;Li et al, 2004;Kumar et al, 2009). In this study, the concentrations of serum IFN-γ in the 0.3 mg·kg -1 ·day -1 BCG-PSN group on days 5 and 25, and in the 0.15 and 0.3 mg·kg -1 ·day -1 BCG-PSN groups on day 15 after vaccination were significantly higher than that in the negative control and vaccine group.…”

Section: Discussionmentioning

confidence: 99%

Mixture of polysaccharide and nucleic acid extracted from Bacillus Calmette-Guerin (BCG) enhances immune response of infectious bursal disease virus vaccine in chickens

Wang

Liu

et al. 2016

Genet. Mol. Res.

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ABSTRACT. In this study, the immune response induced by a mixture of polysaccharide and nucleic acid extracted from Bacillus CalmetteGuerin (BCG) was evaluated in chickens inoculated with infectious bursal disease virus (IBDV) vaccine. After the mixture was injected intramuscularly at a dose of 0.075, 0.15 or 0.3 mg·kg -1 ·day -1 for 3 days, the 14-day-old chickens were inoculated with the attenuated IBDV vaccine via intranasal and ocular routes. The relative weight of bursa of Fabricius (BF) and thymus, the serum IBD antibody titer, the CD4+/CD8+ ratio, and the concentrations of IFN-γ, IL-2 and IL-6 in peripheral blood were investigated on days 5, 15 and 25. The IBD antibody titer in BCG-treated groups was higher than in the negative control and only IBD-vaccinated chickens, indicating that the mixture of BCG can significantly enhance chicken humoral response. CD4+/ CD8+ and the secretions of IFN-γ, IL-2 and IL-6 were also clearly increased compared with that in the negative control and IBD-vaccinated chickens, indicating that the mixture can also enhance the cell-mediated immune response. The results also showed that the relative weights of BF and thymus increased after chickens were inoculated with BCG, indicating that the BCG mixture can clearly enhance the immunity of IBD-vaccine and can be expected to be viewed as a candidate for a new type of immune adjuvant.

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Section: Discussionmentioning

confidence: 99%

Mixture of polysaccharide and nucleic acid extracted from Bacillus Calmette-Guerin (BCG) enhances immune response of infectious bursal disease virus vaccine in chickens

Wang

Liu

et al. 2016

Genet. Mol. Res.

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show abstract

“…Thus, its preparation is simple and cost-efficient and each injection of 0.2 mg of SVP per bird is not cost-prohibitive. In the future, the VP2 N5-452H gene of IBDV can be cloned in a bicistronic vector along with the chicken interleukin-2 (chiIL-2) as an adjuvant for the development of a bicistronic DNA vaccine with extremely effective protection [42,43]. This strategy can also be applied to prevent infection from escaped quasi-species and variants of IBDV.…”

Section: Discussionmentioning

confidence: 99%

Vaccine development through terminal deletions of an infectious bursal disease virus protein 2 precursor variant

Lee

Lin

et al. 2010

Process Biochemistry

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“…VP2, the major structural protein of IBDV, contains antigenic epitopes that are responsible for generation of neutralizing antibodies (Vakharia et al, 1993). To perk up the efficiency of VP2 based DNA vaccine against IBDV, various co-stimulatory molecules have been tried with VP2 such as chicken interleukin-2 (chiIL-2) (Kumar et al, 2009), chicken interferon gamma (ChIFN-γ) chicken interleukin-2 (chiIL-2) (Park et al, 2009) and chicken β-defensin-1 which all showed a varying degree of protection against a lethal IBD infection in chickens.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of a Salmonella typhimurium flagellin based chimeric DNA vaccine against infectious bursal disease of poultry

Deb

Dey

Mohan

et al. 2015

Research in Veterinary Science

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Effective protection by high efficiency bicistronic DNA vaccine against infectious bursal disease virus expressing VP2 protein and chicken IL-2

Cited by 34 publications

References 27 publications

Mixture of polysaccharide and nucleic acid extracted from Bacillus Calmette-Guerin (BCG) enhances immune response of infectious bursal disease virus vaccine in chickens

Mixture of polysaccharide and nucleic acid extracted from Bacillus Calmette-Guerin (BCG) enhances immune response of infectious bursal disease virus vaccine in chickens

Vaccine development through terminal deletions of an infectious bursal disease virus protein 2 precursor variant

Development and evaluation of a Salmonella typhimurium flagellin based chimeric DNA vaccine against infectious bursal disease of poultry

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