Effective protection induced by three different versions of the porcine S3Pvac anticysticercosis vaccine against rabbit experimental Taenia pisiformis cysticercosis

Betancourt, Miguel; Aluja, Aline S. de; Sciutto, Edda; Hernández, Marisela; Bobes, Raúl J.; Rosas, Gabriela; Hernández, Beatríz; Fragoso, Gladis; Hallal-Calleros, Claudia; Aguilar, Liliana; Flores-Pérez, Iván

doi:10.1016/j.vaccine.2012.02.022

Cited by 26 publications

(10 citation statements)

References 32 publications

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“…However, in other studies by the same author, only 57 cysts were found after infection with 2000 eggs (Craig, 1984). Another group reported that an average of 8 cysticerci was recovered after challenge with 3300 eggs (Gemmell, 1965;Betancourt et al, 2012). We found an average of 206 cysticerci after infection with 5000 eggs.…”

Section: Discussioncontrasting

confidence: 45%

“…Recently, the efficacy of the oral vs injectable S3Pva-synthetic and S3Pvac-phage vaccines from Taenia solium were compared. The data showed that oral S3Pvac-papaya significantly reduced the expected number of hepatic lesions and peritoneal cysticerci to a similar extent to that of the injectable vaccines (Betancourt et al, 2012). In the current study, the reduction in the recovery of larvae induced by the TpUbc2 recombinant vaccine was 79.3 and 90.8%.…”

Section: Discussionmentioning

confidence: 84%

“…In previous studies, most researchers have focused on other methods of vaccine production to control cysticercosis, such as recombinant vaccines Jayashi et al, 2012), synthesized peptide vaccines (Huerta et al, 2001;De Aluja et al, 2005;Cruz-Revilla et al, 2006;Gazarian et al, 2012), and phage vaccines (Betancourt et al, 2012). Recently, the efficacy of the oral vs injectable S3Pva-synthetic and S3Pvac-phage vaccines from Taenia solium were compared.…”

Section: Discussionmentioning

confidence: 99%

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Protection against Taenia pisiformis larval infection induced by a recombinant oncosphere antigen vaccine

et al. 2014

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ABSTRACT. Taenia pisiformis larvae cause significant health problems to rabbits. At present, it is not known whether the recombinant antigen from the T. pisiformis oncosphere is able to confer protective immunity against T. pisiformis larval infection. The full-length cDNA was cloned into a pET32a (+) vector, and the recombinant protein was then expressed in BL21 (DE3) cells. Vaccination with the purified rTpUbc2 coupled with QuilA was carried out in New Zealand rabbits to evaluate the immunoprotective effect against T. pisiformis infection. The full-length open reading frame of the TpUbc2 gene was 444 bp, and encoded a 16.63-kDa protein. Finally, rTpUbc2 was used to evaluate the ability to induce immunoprotective responses in rabbits. A 79.3-90.8% reduction (P < 0.01) in the recovery of larvae was observed in the experimental group compared to the control group. Specific anti-rTpUbc2 antibodies from immunized rabbits had significantly higher levels of IgG (P < 0.01) compared to the control group; however, no significant difference in IgA levels was found between groups 6149 Protection against Taenia pisiformis larvae ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6148-6159 (2014) (P > 0.05). Our data support the use of rTpUbc2 as a potential candidate to develop a vaccine against T. pisiformis larvae.

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Section: Discussioncontrasting

confidence: 45%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

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Protection against Taenia pisiformis larval infection induced by a recombinant oncosphere antigen vaccine

et al. 2014

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“…The rabbits were humanely sacrificed (50 mg/kg ketamine and 100 mg/kg sodium pentobarbital [Sigma, San Francisco, USA]) at 49 days post-infection. Necropsies were as performed as previously described [3]. …”

Section: Methodsmentioning

confidence: 99%

Expression and immunolocalisation ofTpFABPas a candidate antigen for the serodiagnosis of rabbitTaenia pisiformiscysticercosis

et al. 2013

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The larval stage of Taenia pisiformis, also known as Cysticercus pisiformis, is the causative agent of cysticercosis and the cause of severe health problems in rabbits that negatively impacts on husbandry production. To date, there is no fast detection method to identify early infections in rabbits. In the present study, a new dot-ELISA-based on an endogenous antigen fatty acid-binding protein (FABP) was developed for the detection of cysticercosis, and its potential was then evaluated using test serum samples. Immunolocalisation showed that T. pisiformis FABP (TpFABP) localised to the parenchyma of the bladder wall of the cysticercus and perinuclear cytoplasm of parenchyma of the adult parasite. After cloning and expression, recombinant TpFABP (rTpFABP) protein was used for serodiagnosis of T. pisiformis infection in rabbits by dot-ELISA. The antibody was detected 14 days post-infection in rabbits experimentally infected with T. pisiformis. Based on the necropsy results, the sensitivity and specificity of 169 serum samples tested by rTpFABP dot-ELISA were found to be 98.2% (54/55) and 92.1% (105/114), respectively. These data suggest that the dot-ELISA developed in this study has potential for detection of T. pisiformis infection in rabbits.

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“…S3Pvac-papaya induces high protection levels against T. crassiceps and T. pisiformis cysticercosis when parenterally or orally administered [20,21]. While this vaccine has proved efficacious, further improvements are envisioned in light of the molecular tools of plant biotechnology currently available [16,22,23].…”

Section: Introductionmentioning

confidence: 99%

Expression of Multiple Taenia Solium Immunogens in Plant Cells Through a Ribosomal Skip Mechanism

et al. 2015

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Taenia solium cysticercosis is a major parasitic disease that affects the human health and the economy in underdeveloped countries. Porcine cysticercosis, an obligatory stage in the parasite life cycle, is a suitable target for vaccination. While several recombinant and synthetic antigens proved to be effective as vaccines, the cost and logistic difficulties have prevented their massive use. Taking this into account, a novel strategy for developing a multi-epitope low-cost vaccine is herein explored. The S3Pvac vaccine components (KETc1, KETc12, KETc7, and GK1 [KETc7]) and the protective HP6/TSOL18 antigen were expressed in a Helios2A polyprotein system, based on the 'ribosomal skip' mechanism mediated by the 2A sequence (LLNFDLLKLAGDVESNPG-P) derived from the Foot-and-mouth disease virus, which induces self-cleavage events at a translational level. This protein arrangement was expressed in transgenic tobacco cells. The inserted sequence and its transcript were detected in several Helios2A lines, with some lines showing recombinant protein accumulation levels up to 1.3 µg/g of fresh weight in leaf tissues. The plant-derived Helios2A vaccine was recognized by antibodies in the cerebral spinal fluid from neurocysticercosis patients and elicited specific antibodies in BALB/c immunized mice. These evidences point to the Helios2A polyprotein as a promising system for expressing multiple antigens of interest for vaccination and diagnosis in one single construction.

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Effective protection induced by three different versions of the porcine S3Pvac anticysticercosis vaccine against rabbit experimental Taenia pisiformis cysticercosis

Cited by 26 publications

References 32 publications

Protection against Taenia pisiformis larval infection induced by a recombinant oncosphere antigen vaccine

Protection against Taenia pisiformis larval infection induced by a recombinant oncosphere antigen vaccine

Expression and immunolocalisation ofTpFABPas a candidate antigen for the serodiagnosis of rabbitTaenia pisiformiscysticercosis

Expression of Multiple Taenia Solium Immunogens in Plant Cells Through a Ribosomal Skip Mechanism

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