Effective release of a broad spectrum antibiotic from elastin‐like polypeptide‐collagen composite

Anderson, Tiffany R.; Marquart, Mary E.; Janorkar, Amol V.

doi:10.1002/jbm.a.35219

Cited by 16 publications

(28 citation statements)

References 53 publications

(107 reference statements)

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“…A simple method of fabrication involves the addition of ELPs to a material that naturally undergoes gelation . ELPs mixed with collagen solutions form hydrogels when incubated at 37 °C with no additional need for cross‐linkers, due to both direct interactions and water‐mediated hydrogen bonding between the two components . ELPs combined with fibrinogen and thrombin form sponges after covalent bonding between ELPs and fibrin monomers generated from thrombin cleavage of fibrinogen …”

Section: Composite Elp‐based Materialsmentioning

confidence: 99%

“…Similarly, hydrophobic interactions between ELP and carbon nanosphere chains enhance the strength of such composite hydrogels . ELP‐collagen hydrogels also have a higher tensile strength arising from stronger intermolecular bonding, and a lower elastic modulus . Hybrid ELP‐PEG hydrogels likewise display lower shear storage and elastic moduli .…”

Section: Composite Elp‐based Materialsmentioning

confidence: 99%

“…Composite ELP‐based hydrogels are utilized in a range of applications. They can be used for drug delivery, particularly in areas that are not amenable to systemic injection or topical application …”

Section: Composite Elp‐based Materialsmentioning

confidence: 99%

“…Hydrogels developed as nerve conduits for peripheral nerve regeneration support Schwann cell adhesion and proliferation . Accordingly, these hydrogels can also entrap cells with high viability, due to their high water content, cytocompatibility, and extracellular matrix‐mimetic structure and mechanics . This was demonstrated when cells mixed with solubilized ELP‐collagen were encapsulated within the matrix of the resulting hydrogel .…”

Section: Composite Elp‐based Materialsmentioning

confidence: 99%

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Fabricated Elastin

Yeo

Aghaei-Ghareh-Bolagh

Brackenreg

et al. 2015

Adv Healthcare Materials

103

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The mechanical stability, elasticity, inherent bioactivity, and self-assembly properties of elastin make it a highly attractive candidate for the fabrication of versatile biomaterials. The ability to engineer specific peptide sequences derived from elastin allows for precise control of these physicochemical and organizational characteristics, and further broadens the diversity of elastin-based applications. Elastin and elastin-like peptides can also be modified or blended with other natural or synthetic moieties, including peptides, proteins, polysaccharides and polymers, to augment existing capabilities or confer additional architectural and biofunctional features to compositionally pure materials. Elastin and elastin-based composites have been subjected to diverse fabrication processes, including heating, electrospinning, wet spinning, solvent casting, freeze-drying, and cross-linking, for the manufacture of particles, fibers, gels, tubes, sheets and films. The resulting materials can be tailored to possess specific strength, elasticity, morphology, topography, porosity, wettability, surface charge and bioactivity. This extraordinary tunability of elastin-based constructs enables their use in a range of biomedical and tissue engineering applications such as targeted drug delivery, cell encapsulation, vascular repair, nerve regeneration, wound healing, and dermal, cartilage, bone and dental replacement.

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Section: Composite Elp‐based Materialsmentioning

confidence: 99%

Section: Composite Elp‐based Materialsmentioning

confidence: 99%

Section: Composite Elp‐based Materialsmentioning

confidence: 99%

Section: Composite Elp‐based Materialsmentioning

confidence: 99%

See 2 more Smart Citations

Fabricated Elastin

Yeo

Aghaei-Ghareh-Bolagh

Brackenreg

et al. 2015

Adv Healthcare Materials

103

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“…Previous work has shown that antibioticloaded collagen scaffolds, 47 composite fibers comprised of polyglyconate and porous poly(DLlactic-co-glycolic acid) (PDLGA), 48,49 and electrospun nanofibers consisting of biodegradable poly vinyl alcohol and sodium alginate, 50 can reduce infection either in vitro or in vivo. Recently, Albaugh et al combined topical antibiotics with cellulose dressing to fight infection of lower extremity chronic ulcers infected with MRSA.…”

Section: Discussionmentioning

confidence: 99%

Ciprofloxacin-Loaded Keratin Hydrogels PreventPseudomonas aeruginosaInfection and Support Healing in a Porcine Full-Thickness Excisional Wound

Roy

Tomblyn

Burmeister

et al. 2015

Advances in Wound Care

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Objective: Cutaneous wound infection can lead to impaired healing, multiple surgical procedures, and increased hospitalization time. We tested the effectiveness of keratin-based hydrogels (termed ''keratose'') loaded with ciprofloxacin to inhibit infection and support healing when topically administered to porcine excision wounds infected with Pseudomonas aeruginosa. Approach: Using a porcine excisional wound model, 10 mm full-thickness wounds were inoculated with 10 6 colony-forming units of P. aeruginosa and treated on days 1 and 3 postinoculation with ciprofloxacin-loaded keratose hydrogels. Bacteria enumeration and wound healing were assessed on days 3, 7, and 11 postinjury. Results: Ciprofloxacin-loaded keratose hydrogels reduced the amount of P. aeruginosa in the wound bed by 99.9% compared with untreated wounds on days 3, 7, and 11 postinjury. Ciprofloxacin-loaded keratose hydrogels displayed decreased wound contraction and reepithelialization at day 7 postinjury. By day 11, wounds treated with ciprofloxacin-keratose hydrogels contained collagen-rich granulation tissue and myofibroblasts. Wounds treated with ciprofloxacin-loaded keratose hydrogels exhibited a transient increase in macrophages in the wound bed at day 7 postinjury that subsided by day 11. Innovation: Current therapies for wound infection include systemic antibiotics, which could lead to antibiotic resistance, and topical antimicrobial treatments, which require multiple applications and can delay healing. Here, we show that ciprofloxacin-loaded keratose hydrogels inhibit cutaneous wound infection without interfering with key aspects of the healing process including granulation tissue deposition and remodeling. Conclusions: Ciprofloxacin-loaded keratose hydrogels have the potential to serve as a point-of-injury antibiotic therapy that prevents infection and supports healing following cutaneous injury.

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