Effective utilization of dichloromethane by a newly isolated strain Methylobacterium rhodesianum H13

Chen, Dongzhi; Ouyang, Du-Juan; Liu, Hongxia; Chen, Jing; Zhuang, Qing-Feng; Chen, Jianmeng

doi:10.1007/s11356-013-1972-7

Cited by 15 publications

(3 citation statements)

References 28 publications

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“…These regions may help to enhance adaptability and adhesion of the strain MB200 to surrounding environment ( Supplementary Table S2 ). The capacity to grow in the presence of dichloromethane (DCM) has been demonstrated in M. rhodesianum H13 [ 22 ]. However, the strain MB200 cannot grow on DCM, and the dcm gene cluster was not present in the genome of the strain MB200.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis of a Methylorubrum rhodesianum MB200 Isolated from Biogas Digesters Provided New Insights into the Carbon Metabolism of Methylotrophic Bacteria

Zhang

Xia

Wang

et al. 2023

IJMS

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Methylotrophic bacteria are widely distributed in nature and can be applied in bioconversion because of their ability to use one-carbon source. The aim of this study was to investigate the mechanism underlying utilization of high methanol content and other carbon sources by Methylorubrum rhodesianum strain MB200 via comparative genomics and analysis of carbon metabolism pathway. The genomic analysis revealed that the strain MB200 had a genome size of 5.7 Mb and two plasmids. Its genome was presented and compared with that of the 25 fully sequenced strains of Methylobacterium genus. Comparative genomics revealed that the Methylorubrum strains had closer collinearity, more shared orthogroups, and more conservative MDH cluster. The transcriptome analysis of the strain MB200 in the presence of various carbon sources revealed that a battery of genes was involved in the methanol metabolism. These genes are involved in the following functions: carbon fixation, electron transfer chain, ATP energy release, and resistance to oxidation. Particularly, the central carbon metabolism pathway of the strain MB200 was reconstructed to reflect the possible reality of the carbon metabolism, including ethanol metabolism. Partial propionate metabolism involved in ethyl malonyl-CoA (EMC) pathway might help to relieve the restriction of the serine cycle. In addition, the glycine cleavage system (GCS) was observed to participate in the central carbon metabolism pathway. The study revealed the coordination of several metabolic pathways, where various carbon sources could induce associated metabolic pathways. To the best of our knowledge, this is the first study providing a more comprehensive understanding of the central carbon metabolism in Methylorubrum. This study provided a reference for potential synthetic and industrial applications of this genus and its use as chassis cells.

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Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis of a Methylorubrum rhodesianum MB200 Isolated from Biogas Digesters Provided New Insights into the Carbon Metabolism of Methylotrophic Bacteria

Zhang

Xia

Wang

et al. 2023

IJMS

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“…Methylene chloride had the highest content. Methylene chloride has a sweet taste and chloroform-like odor (Wang et al, 2022), is prevalent in water, and is toxic and a hazardous compound (Chen et al, 2013;Hammoud et al, 2021). Hendra et al (2021) extracted dichloromethane from dragon fruit peel and showed that dichloromethane has high antiplasmodial activity.…”

Section: Volatile Substances In Sv Crayfish Meat During Refrigerationmentioning

confidence: 99%

Analysis of storage quality of crayfish (Procambarus clarkii) meat based on sous-vide cooking

ZHAO,

LI,

CAI

et al. 2023

Food Sci. Technol (Campinas)

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This study aimed to investigate the effect of different combinations of temperature and time of sous-vide (SV) on the quality of crayfish (Procambarus clarkii) meat. The changes in quality indexes and volatile components of crayfish meat after SV treatment were analyzed after refrigeration at 4°C for 1–7 days. The L* values of crayfish meat were higher in the SV group than in the control group (p<0.05). Heating at 75℃ effectively suppressed the decrease in hardness and increase in total volatile basic nitrogen (TVB-N) and total viable count (TVC) of crayfish meat (p<0.05). A heating time of 15 min was optimal for most of the indicators. TVB-N, thiobarbituric acid, and TVC were positively correlated with each other and negatively correlated with hardness. Using GC-MS, 59 volatile compounds were identified with a match of >85%. During storage, the highest percentages of volatile alkanes and aromatic hydrocarbons were 44.57 and 14.81%, respectively, with 33 species of aromatic hydrocarbons being identified. In conclusion, SV treatment with a heating temperature of 75℃ and heating time of 15 min improved the texture and flavor quality of crayfish meat during storage.

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“…GSTs with known crystal structures can be used as templates for the prediction of DCM dehalogenase catalytic mechanism [23][24][25]. In our previous work, the DCM dehalogenase gene sequence was obtained from M. rhodesianum H13, which is a highly efficient DCM-degrading bacterium [26]. The specific activity of DCM dehalogenase from recombinant bacteria Escherichia coli BL21(DE3)-pET28b(þ)-dcm was detected as 0.00686 ± 0.00005 U/ mg, three times greater than from wild-type M. rhodesianum [27].…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics, bacterial expression and enzyme activity analyses of dichloromethane dehalogenase fromMethylobacterium rhodesianumH13

Liu

Shen

et al. 2020

Biotechnology & Biotechnological Equipment

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Dechlorination is the key step in biological degradation of dichloromethane (DCM), which is catalyzed by the enzyme DCM dehalogenase. In this study, the detailed structure and function of DCM dehalogenase in Methylobacterium rhodesianum H13 were investigated. Our results indicate that DCM dehalogenase (C1505H2255N403O430S7) is a stable hydrophilic protein localized in the cytoplasm, with an isoelectric point of 6.35. They also indicate that DCM dehalogenase is a frizzled protein with 52.43% of amino acid residues exposed on its surface, and that alpha-helices are a main structural component (53.47%). As one of the theta class glutathione S-transferases (GSTs), DCM dehalogenase presents as a dimeric structure and shows strong substrate selectivity. The catalytic tunnel radius and length of DCM dehalogenase are both 1.4 Å. Glu42 and Val43 are two of the essential amino acids in both the catalytic tunnel and catalytic pockets. The amino acid sequence of DCM dehalogenase was predicted to be homologous among various microorganisms, and its active site was conserved with eight potential sites. The recombinant DCM dehalogenase was expressed in E. coli BL21 strain. The optimum conditions for DCM dehalogenase are pH 8, temperature of 30 C and presence of Mn 2þ. All the obtained information of DCM dehalogenase will lay the foundation for further study of its structural transformation and functional improvement.

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Effective utilization of dichloromethane by a newly isolated strain Methylobacterium rhodesianum H13

Cited by 15 publications

References 28 publications

Comparative Genomic Analysis of a Methylorubrum rhodesianum MB200 Isolated from Biogas Digesters Provided New Insights into the Carbon Metabolism of Methylotrophic Bacteria

Comparative Genomic Analysis of a Methylorubrum rhodesianum MB200 Isolated from Biogas Digesters Provided New Insights into the Carbon Metabolism of Methylotrophic Bacteria

Analysis of storage quality of crayfish (Procambarus clarkii) meat based on sous-vide cooking

Bioinformatics, bacterial expression and enzyme activity analyses of dichloromethane dehalogenase fromMethylobacterium rhodesianumH13

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