Effectiveness of ciprofloxacin microspheres in eradicating bacterial biofilm

Owusu-Ababio, Godfried

doi:10.1016/s0168-3659(98)00113-8

Cited by 14 publications

(10 citation statements)

References 26 publications

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“…Encapsulating low molecular weight drugs into NPs has been shown to retard drug clearance from tissues [31,32]. Furthermore, studies by Owusu-Ababio et al have shown that NPs are effective for delivering antimicrobial drugs into biofilm infections [33]. To effectively treat biofilms in small airways, NPs must be small enough to navigate through the respiratory tract without impaction in the nasopharynx, while large enough to deposit onto the matrix of the biofilms [34–36].…”

Section: Resultsmentioning

confidence: 99%

The antimicrobial efficacy of sustained release silver–carbene complex-loaded l-tyrosine polyphosphate nanoparticles: Characterization, in vitro and in vivo studies

et al. 2009

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The pressing need to treat multi-drug resistant bacteria in the chronically infected lungs of cystic fibrosis (CF) patients has given rise to novel nebulized antimicrobials. We have synthesized a silver–carbene complex (SCC10) active against a variety of bacterial strains associated with CF and chronic lung infections. Our studies have demonstrated that SCC10-loaded into l-tyrosine polyphosphate nanoparticles (LTP NPs) exhibits excellent antimicrobial activity in vitro and in vivo against the CF relevant bacteria Pseudomonas aeruginosa. Encapsulation of SCC10 in LTP NPs provides sustained release of the antimicrobial over the course of several days translating into efficacious results in vivo with only two administered doses over a 72 h period.

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Section: Resultsmentioning

confidence: 99%

The antimicrobial efficacy of sustained release silver–carbene complex-loaded l-tyrosine polyphosphate nanoparticles: Characterization, in vitro and in vivo studies

et al. 2009

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“…Also, patients can not be noncompliant to medical treatment and miss doses, as it frequently happens when on prolonged oral therapy. Moreover, hospitalization time and costs related to the treatment may be reduced [2,4,8,10, 1 4,18,19,27,3 5,3 71. In the present study, S. aureus was chosen to produce a reliable and reproducible model of osteomyelitis because it has been previously used as a standard [13][14][15][27][28][29]33,35,37]. Nevertheless, quinolone resistance has become a concern among clinical isolates of S. uureus [24,27,30].…”

Section: Discussionmentioning

confidence: 99%

“…strains [23,34]. Although the performance of ciprofloxacin in treating experimental infections has been questioned [24,27,30], it has been reported that this agent is very active against the biofilm of S. aureus and P. aeruginosa [33,36], and that it may be a judicious option for antibiotic therapy of osteomyelitis [23,34]. Consequently, CLHAS implants containing ciprofloxacin may have a potential as a controlled DDS for prevention and treatment of musculoskeletal infections.…”

Section: Introductionmentioning

confidence: 99%

Prevention and treatment of experimental osteomyelitis in dogs with ciprofloxacin‐loaded crosslinked high amylose starch implants

Huneault

Lussier

Dubreuil

et al. 2004

Journal Orthopaedic Research

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Crosslinked high amylose starch (CLHAS) matrix was used as a biodegradable drug delivery implant for the prevention and treatment of osteomyelitis. Thirty-two dogs underwent the femoral insertion of a screw inoculated with Staphylococcus aureus and were then randomly assigned to four groups: (A) prevention with ciprofloxacin-CLHAS implants, (B) surgical debridement (positive control), (C) surgical debridement and oral ciprofloxacin treatment and (D) surgical debridement and treatment with ciprofloxacin-CLHAS implants. At week 4 the osteomyelitis was confirmed, the infected site debrided and respective treatments initiated for groups B, C and D. Radiographs, macroscopic evaluations, bacterial cultures and histopathological examinations were used to evaluate the femora at week 10. Femora from preventive group A were almost normal. Dogs of both ciprofloxacin treatment groups C and D showed better bone healing, less periosteal reaction and less screw mobility than dogs from group B. Eradication of infection was observed at proximal/distal sites in B: 25%/12%, C: 37%/62% and D: 62%/75%. Both ciprofloxacin treated groups improved radiographically from week 4 to week 10. Periosteal and marrow neutrophilic and lymphoplasmocytic infiltrations were less severe in groups C and D versus group B. These data suggest that biodegradable ciprofloxacin-CLHAS implants are a safe and efficient modality for the prevention and treatment of osteomyelitis.

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“…17 However, the in vivo efficacy of the SCCs alone may be limited by their rapid clearance, which is typical for small molecule drugs. 21–23 Moreover, silver compounds can react with and be inactivated by sulfur-containing proteins and chloride ions that are present in the bloodstream. 22, 24 Therefore, the development of an appropriate drug delivery system to carry the SCCs should significantly decrease their clearance rate from the body, and could provide protection from external agents present in the bloodstream, increasing the stability and efficacy of the SCCs.…”

Section: Introductionmentioning

confidence: 99%

Aerosolized Antimicrobial Agents Based on Degradable Dextran Nanoparticles Loaded with Silver Carbene Complexes

et al. 2012

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Degradable acetalated dextran (Ac-DEX) nanoparticles were prepared and loaded with a hydrophobic silver carbene complex (SCC) by a single-emulsion process. The resulting particles were characterized for morphology and size distribution using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The average particle size and particle size distribution were found to be a function of the ratio of the organic phase to the surfactant containing aqueous phase with a 1:5 volume ratio of Ac-DEX CH2Cl2 (organic): PBS (aqueous) being optimal for the formulation of nanoparticles with an average size of 100 ± 40 nm and a low polydispersity. The SCC loading was found to increase with an increase in the SCC quantity in the initial feed used during particle formulation up to 30% (w/w); however, the encapsulation efficiency was observed to be the best at a feed ratio of 20% (w/w). In vitro efficacy testing of the SCC loaded Ac-DEX nanoparticles demonstrated their activity against both Gram-negative and Gram-positive bacteria; the nanoparticles inhibited the growth of every bacterial species tested. As expected, a higher concentration of drug was required to inhibit bacterial growth when the drug was encapsulated within the nanoparticle formulations compared with the free drug illustrating the desired depot release. Compared with free drug, the Ac-DEX nanoparticles were much more readily suspended in an aqueous phase and subsequently aerosolized, thus providing an effective method of pulmonary drug delivery.

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Effectiveness of ciprofloxacin microspheres in eradicating bacterial biofilm

Cited by 14 publications

References 26 publications

The antimicrobial efficacy of sustained release silver–carbene complex-loaded l-tyrosine polyphosphate nanoparticles: Characterization, in vitro and in vivo studies

The antimicrobial efficacy of sustained release silver–carbene complex-loaded l-tyrosine polyphosphate nanoparticles: Characterization, in vitro and in vivo studies

Prevention and treatment of experimental osteomyelitis in dogs with ciprofloxacin‐loaded crosslinked high amylose starch implants

Aerosolized Antimicrobial Agents Based on Degradable Dextran Nanoparticles Loaded with Silver Carbene Complexes

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