Effectiveness of different methods for anti-Sm antibody identification. A multicentre study

Abstract

Methods for the measurement of autoantibodies frequently provide controversial results. The objective of the present study was to evaluate the performance of Spanish Clinical Laboratories in the measurement of anti-Sm antibodies. A total of 23 laboratories participated, analysing 30 serum samples from patients with systemic lupus erythematosus and other autoimmune and non-autoimmune diseases. The laboratories used four extractable nuclear antigen screens, eight enzyme-linked immunosorbent assays (ELISAs) specific for anti-Sm, one line-blot, one dot-blot and one double immunodiffusion assay, from 15 different manufacturers. A total of 871 results were obtained. In general, very good sensitivity was obtained (95-100%), but specificity was moderate (52-86%) and must be improved. Most ELISAs and the line-blot were valid assays for anti-Sm detection and could serve as tests both for analysis and/or confirmation. The likelihood ratios indicated that both methods can be considered very useful or useful for the determination of anti-Sm antibodies. Nevertheless, the analytical quality of the methods for the measurement of anti-Sm antibodies could probably be improved by standardisation of the methods and the participation of laboratories in external quality control programs.

Biosensors for Biological Warfare Agent Detection

“…The conventional detection methods are based on selective cultivation, 44 . Immunological routine methods for pathogen detection such as ELISA 45,46 , dot immunobindig assay (DIA) 47 , electrochemiluminiscence (ECL) 48 , and time-resolved fluorescence (TRF) 49 were employed.…”

Performance evaluation of a laboratory‐developed light‐initiated chemiluminescence assay for quantification of egg white‐specific IgE

“…Compared with Dot-ELISA, ELISA is more suitable for quantitative analysis. 14 The disadvantage is that due to the limitation of ELISA plate coating area, high amount of total IgE (tIgE) may affect the binding of sIgE antibody so that influence the sensitivity of analysis. ImmunoCAP system has strong anti-interference capability to allergen-specific IgG antibodies.…”

“…The use of avidin and biotin systems can amplify signal and then improve the analytical sensitivity. Compared with Dot‐ELISA, ELISA is more suitable for quantitative analysis 14 . The disadvantage is that due to the limitation of ELISA plate coating area, high amount of total IgE (tIgE) may affect the binding of sIgE antibody so that influence the sensitivity of analysis.…”

Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor

“…The dot immunobinding assay was used for characterization of monoclonal antibodies against the laminin receptor [7], mycobacterial antigen [8] and serotypization [9]. From the biosensor-based tools, surface plasmon resonance (SPR) is convenient for observation of immunological interactions [10].…”

“…Classical methods for measurement and characterization of antibodies include immunological assays such as agglutination tests, enzyme-linked immunosorbent assay (ELISA), western blot, immunofluorescence and flow cytometry [ 6 ]. The dot immunobinding assay was used for characterization of monoclonal antibodies against the laminin receptor [ 7 ], mycobacterial antigen [ 8 ] and serotypization [ 9 ]. From the biosensor-based tools, surface plasmon resonance (SPR) is convenient for observation of immunological interactions [ 10 ].…”