Effectiveness of Gamma Rays in Attenuating Rodent Malaria Parasites of &lt;em&gt;Plasmodium berghei&lt;/em&gt; in Blood of Mice

Syaifudin, Mukh; Darlina, Darlina; Rahardjo, Tur; Tetriana, Devita; Nurhayati, Siti; Surniyantoro, Harry Nugroho Eko; Kisnanto, Teja

doi:10.17146/aij.2013.219

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…A serial research on malaria vaccine had been done in the Center where the main focus, according to tasks, is the determination of dose and dose rate of gamma rays that is most effective in attenuating P. berghei, either for pre-erythrocytic-or erythrocytic-stage malaria parasites (Syaifudin et al, 2011;Syaifudin et al, 2013). These are considered by several aspects such as hematopoietic, histopathologic, immunologic and genetics.…”

Section: Introductionmentioning

confidence: 99%

Irradiation of intraerythrocytic Plasmodium berghei with a fractionated dose of gamma rays does not effectively reduce the infectivity in mice Mus musculus

et al. 2019

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Malaria infection kills more than one million human every year, mainly under-5-year-old children, including in South East Asian nations. Gamma radiation given at a single dose is commonly used to create the attenuated Plasmodium parasites to get vaccine materials. However, there is no study on the infectivity of parasites after fractionated γ-radiation. This study aimed to assess the infectivity of parasites after irradiated with fractionated γ-rays in mice. A number of Plasmodium bergheithat was irradiated in two fractions of 100 and 50 Gy, 100 and 75 Gy; and 100 and 100 Gy within 5 minutes of interval time was injected intraperitoneally into 12 mice. Mice injected with unirradiated parasites (0 Gy) served as a control group. The parasitemia level of intraerythrocytic parasites in each group was observed at days post injection up to 20 days by making Giemsa stained thin blood smears and observed under the microscope. Results showed that fractionation radiation did not effectively attenuate the parasites where they still grew in blood of mice, except for 100+75 Gy. There are no significant differences among the treatment groups (p0.05). This is different from irradiation at the single dose that resulted in almost completely attenuated parasites mainly the dose of 150 Gy. This implicating that irradiation of gamma rays at a single dose is a better way to mitigate parasites than fractionation dose as the infectivity of irradiated parasites were lower compared to that of fractionated dosage. Keywords: Malaria vaccine, Gamma radiation, Fractionation, Parasitemia

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Section: Introductionmentioning

confidence: 99%

Irradiation of intraerythrocytic Plasmodium berghei with a fractionated dose of gamma rays does not effectively reduce the infectivity in mice Mus musculus

et al. 2019

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“…Utilization of nuclear techniques has been widely applied in various fields. Besides being used in sterile B I O D I V E R S I T A S 20 (3): 893-899, March 2019 894 insect techniques (Dyck et al 2005;Ernawan et al 2017;Ernawan et al 2018), nuclear techniques can also be used in attenuating rodent malaria parasites of Plasmodium berghei in blood of mice (Syaifudin et al 2013) and inhibition of Plasmodium falciparum multiplication supplemented with tritium-labeled hypoxanthine (Surniyantoro et al 2016). Ae.…”

Section: Introductionmentioning

confidence: 99%

A mutant variant of Nix gene on γ-irradiated Aedes aegypti as a male-determination factor

et al. 2019

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Abstract. Ernawan B, Surniyantoro HNE, Sugoro I, Tambunan USF. 2019. A mutant variant of Nix gene on γ-irradiated Aedes aegypti as a male-determination factor. Biodiversitas 20: 893-899. This study was aimed to examine the effect of γ-irradiation on the Nix gene sequence as a male-determination factor on Ae. aegypti. The novelty of the present study is the investigation on the gene mutation level of the deoxyribonucleic acid as an impact of ionizing radiation exposure. Males Ae. aegypti at the pupal stage were sterilized by applying 70 Gy γ-rays in varies dose-rates, i.e., 300, 600, 900, 1200 and 1500 Gy/h utilizing panoramic irradiator and males group without γ-irradiation as controls. Adult males that emerged from the pupal stage were assessed for the Nix gene sequence using a Real-Time Polymerase Chain Reaction method and were sequenced using Clustal Omega and Bioedit software, respectively. The results indicated that mutation occurred in Nix gene sequences were categorized as a point mutation. The change of Nix gene sequences was occurred between the nitrogenous base of guanine to adenine (G>A), thymine to adenine (T>A) and cytosine to adenine (C>A) in certain sequence numbers. There were six and nineteen on the forward-strand and reverse-strand of the Nix gene, respectively, due to γ-irradiation treatment. We conclude that γ-irradiation exposure causes point mutation on the Nix gene of males Ae. aegypti.

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Blastocystis hominis undergoing programmed cell death via cytotoxic gamma irradiation

Mohammad

Eldin

Tawfik

et al. 2022

Experimental Parasitology

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Effectiveness of Gamma Rays in Attenuating Rodent Malaria Parasites of <em>Plasmodium berghei</em> in Blood of Mice

Cited by 3 publications

References 15 publications

Irradiation of intraerythrocytic Plasmodium berghei with a fractionated dose of gamma rays does not effectively reduce the infectivity in mice Mus musculus

Irradiation of intraerythrocytic Plasmodium berghei with a fractionated dose of gamma rays does not effectively reduce the infectivity in mice Mus musculus

A mutant variant of Nix gene on γ-irradiated Aedes aegypti as a male-determination factor

Blastocystis hominis undergoing programmed cell death via cytotoxic gamma irradiation

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