Effectiveness of Interventions to Modulate the Rumen Microbiota Composition and Function in Pre-ruminant and Ruminant Lambs

Saro, Cristina; Hohenester, Ulli Martin; Bernard, Mickaël; Lagrée, Marie; Martin, Cécile; Doreau, M.; Boudra, Hamid; Popova, Milka; Morgavi, Diego P.

doi:10.3389/fmicb.2018.01273

Cited by 46 publications

(43 citation statements)

References 50 publications

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“…This agrees with Martinez-Fernandez et al (2015) who observed that the abundances of Methanosphaera were increased in anti-methanogenic organosulphur supplementation lambs [45]. Saro et al (2018) reported that greater abundance of Methanosphaera and lower abundance of Methanomassiliicoccaceae in the rumen of low methaneemitting lambs treated with a combination of garlic essential oil and linseed oil, which partially agrees with our results [46]. It has also been reported that the genus Methanosphaera was H2 utilizing species within the rumen [47].…”

Section: Discussionsupporting

confidence: 93%

Garlic Skin Induced Shifts in the Rumen Microbiome and Metabolome of Fattening Sheep

Zhu

et al. 2020

Preprint

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Background: Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This experiment was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, and involved mechanism in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results: The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. Besides, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions: Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

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Section: Discussionsupporting

confidence: 93%

Garlic Skin Induced Shifts in the Rumen Microbiome and Metabolome of Fattening Sheep

Zhu

et al. 2020

Preprint

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“…Methanosphaera, a methanogen capable to utilize methylated substrates like the Methanomassiliicoccaceae, increased in the GAS group, which agrees with the results observed by Martinez-Fernandez et al [34]. Saro et al [35] reported the greater abundance of Methanosphaera and lower abundance of Methanomassiliicoccaceae in the rumen of low methane-emitting lambs treated with a combination of garlic essential oil and linseed oil, which partially agrees with our results. It has also been reported that the genus Methanosphaera was H 2 utilizing species within the rumen [36].…”

Section: Discussionsupporting

confidence: 93%

Garlic skin induced shifts in the rumen microbiome and metabolome of fattening sheep

Zhu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This study was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, ruminal microbes and metabolites in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. In addition, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

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“…The early life of young ruminant animals provides a unique window to potentially program the development of the rumen microbiota [19,33,34]. Inoculation of adult rumen microbiota to young ruminants was thought to be an effective approach to reprogram.…”

Section: Discussionmentioning

confidence: 99%

Repeated inoculation with fresh rumen fluid before or during weaning modulates the microbiota composition and co-occurrence of the rumen and colon of lambs

Zhang

Liu

et al. 2020

BMC Microbiol

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Background: Many recent studies have gravitated towards manipulating the gastrointestinal (GI) microbiome of livestock to improve host nutrition and health using dietary interventions. Few studies, however, have evaluated if inoculation with rumen fluid could effectively reprogram the development of GI microbiota. We hypothesized that inoculation with rumen fluid at an early age could modulate the development of GI microbiota because of its low colonization resistance. Results:In this study, we tested the above hypothesis using young lambs as a model. Young lambs were orally inoculated repeatedly (four times before or twice during gradual weaning) with the rumen fluid collected from adult sheep. The oral inoculation did not significantly affect starter intake, growth performance, or ruminal fermentation. Based on sequencing analysis of 16S rRNA gene amplicons, however, the inoculation (both before and during weaning) affected the assemblage of the rumen microbiota, increasing or enabling some bacterial taxa to colonize the rumen. These included operational taxonomic units (OTUs) belonging to Moryella, Acetitomaculum, Tyzzerella 4, Succiniclasticum, Prevotella 1, Lachnospiraceae, Christensenellaceae R-7 group, Family XIII AD3011, and Bacteroidales S24-7 corresponding to inoculation before weaning; and OTUs belonging to Succiniclasticum, Prevotellaceae UCG-003, Erysipelotrichaceae UCG-004, Prevotella 1, Bacteroidales S24-7 gut group uncultured bacterium, and candidate Family XIII AD3011 corresponding to inoculation during weaning. Compared to the inoculation during weaning, the inoculation before weaning resulted in more co-occurrences of OTUs that were exclusively predominant in the inoculum. However, inoculation during weaning appeared to have more impacts on the colonic microbiota than the inoculation before weaning. Considerable successions in the microbial colonization of the GI tracts accompanied the transition from liquid feed to solid feed during weaning.Conclusions: Repeated rumen fluid inoculation during early life can modulate the establishment of the microbiota in both the rumen and the colon and co-occurrence of some bacteria. Oral inoculation with rumen microbiota may be a useful approach to redirect the development of the microbiota in both the rumen and colon.

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Effectiveness of Interventions to Modulate the Rumen Microbiota Composition and Function in Pre-ruminant and Ruminant Lambs

Cited by 46 publications

References 50 publications

Garlic Skin Induced Shifts in the Rumen Microbiome and Metabolome of Fattening Sheep

Garlic Skin Induced Shifts in the Rumen Microbiome and Metabolome of Fattening Sheep

Garlic skin induced shifts in the rumen microbiome and metabolome of fattening sheep

Repeated inoculation with fresh rumen fluid before or during weaning modulates the microbiota composition and co-occurrence of the rumen and colon of lambs

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