Effectiveness of wound healing using the novel collagen dermal substitute INSUREGRAF®

Abstract

Collagen sponges are often used as dermal substitutes in the treatment of burns, trauma, infections, and wounds. Dermal substitutes that can be applied in a one-stage operation are particularly important for dermal regeneration. Some protocols for the production of collagen sponges have been developed, but many issues remain, including low yield, contraction, and expense. In this study, the effectiveness of two skin substitutes was evaluated. Specifically, we compared two thin matrices, i.e., the newly developed INSUREGRAF® 1.2 mm and the widely used Matriderm® 1 mm Single Layer, with respect to their biochemical and mechanical properties, safety, and efficacy. We examined the rate of contractibility and biocompatibility using in vitro and in vivo models. The INSUREGRAF had an interconnected pore structure, which affects cell attachment and proper vascularization. Accordingly, this novel collagen sponge type has the potential to promote skin tissue regeneration and is especially suitable for fullthickness skin defects as a one-stage operation substitute.

Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies

“…In terms of material structure, SEM images showed that the sponge of PELNAC™ is different to the matrix of MatriDerm ® . Both materials are highly porous, 80–95% porosity for PELNAC™ and 98.20% porosity for MatriDerm ® according to the literature [11,21]. The two scaffolds have pores of different sizes (70–110 µm for PELNAC™ and 25–60 µm for MatriDerm ® ) that are interconnected.…”

Enhancement of wound healing efficiency mediated by artificial dermis functionalized with EGF or NRG1

“…Recently, INSUREGRAF ® was developed as a OSDS. However, there are still hurdles in that INSUREGRAF ® cannot perfectly heal the wound without scarring in severe skin damage [23]. For this purpose, we attempted to fabricate an efficient artificial dermis functionalized with growth factors to promote wound repair.…”

“…The type I atelo collagen solution (Matrixen-PSC, Bioland), which was used to fabricate INSUREGRAF ® as previously described [23], was derived from porcine skin. This solution was used to fill a copper mold to maintain the structure of the dermal substitutes.…”

“…For example, skin scaffolds (termed as skin substitutes or artificial dermis) including INSUREGRAF ® (South Korea), Matriderm ® (Germany), Pelnac ® (UK), and Integra ® (USA) have been developed as skin wound dressings and applied clinically for eventual scar-free wound healing [21,22]. In particular, the recently developed artificial dermis INSUREGRAF ® is composed of collagen, which has multiple desirable characteristics such as biocompatibility, an interconnected pore structure, and sufficient biodegradability, and is consequently able to regenerate skin tissue as a onestage dermal substitute (OSDS) operation in patients with full-thickness (FT) skin defects [23]. However, INSUREGRAF ® cannot effect scar-free wound healing in cases of severe skin damage such as FT excision.…”

Engineering Bioactive Scaffolds for Skin Regeneration

“…[134] They similarly present desired WUR for wound healing. [128] A moist wound bed needs to ameliorate the dermal reconstruction and wound closure, which result from the scaffold property of water vapor permeability (WVP). Importantly, the WVP is linked to two parameters, the thickness, and area to water surface area ratio.…”