Effectivity of 5% Temulawak Extract (Curcuma xanthorrhiza) on Post-Extraction Fibroblast Cells in Wistar Rats (Rattus norvegicus)

Abstract

Introduction: Socket wound would undergo 3 phases of wound-healing process, i.e. inflammation phase, proliferation phase, and re-modelling phase. In proliferation phase, fibroblast cells were important for producing collagen and elastic cells. One of natural ingredients believed to stimulate fibroblasts is Curcuma xanthorriza. The active compounds in curcuma, namely curcumin and flavonoid, had the ability to increase fibroblast migration and accelerate wound healing. This study aims to observe the effectivity of 5% temulawak extract (Curcuma xanthorrhiza) on post-extraction fibroblast cells in Wistar rats (Rattus norvegicus). Methods: The experiment was conducted on 30 rats that were divided into 2 groups, control group and experimental group. Each group of rats were given intermuscular anesthesia and underwent mandibular incisors extraction. The experiment group was given intermuscular injection of 5% curcuma extract into their wound socket, while control group were given placebo gel injection. On day 3, 5, and 7 decapitation was performed to get the mandibular samples. Furthermore, the number of fibroblast cells was observed using a light microscope with 3 different fields of view at 400 times. Results: The results showed that the mean number of fibroblasts in the experiment group was higher than the control group. Conclusion: 5% temulawak extract (Curcuma xanthorrhiza) had significant effect on increasing post-extraction fibroblast cells in Wistar rats (Rattus norvegicus).