Effectivity of Preovulation Phase Autologous Serum Application on Moderate-Severe Acne Vulgaris Patients

Abstract

Acne vulgaris (AV) is the most common skin disease, including in Indonesia. This research aims to determine the effectivity of preovulation phase autologous serum application in patients with moderate-severe AV. This research was conducted in Makassar, South Sulawesi. Thirteen patient samples with moderate-severe AV who have met the inclusion criteria were taken from Dr. Wahidin Sudirohusodo General Hospital, Public Senior High School, and Medical Faculty of Hasanuddin University Laboratory. The method used is clinical trial. The examination was done by observing the clinical manifestations, sebum level using 815 sebumeter, and estradiol level examination. All patiens were given the same autologous serum treatment which is applied once a day every night and followed-up on day 21 and day 42. The data were processed using paired t test and Fisher's exact test. The result showed that there were improvements in clinical manifestation and decreased sebum level after autologous serum application (p < 0.05) on day 21 compared to day 1, on day 42 compared to day 21, and day 42 compared to day 1. Preovulation estradiol level were higher than the estradiol level on menstruation or in lutheal phase (p < 0.05). There were no significant relationship between routine activity, familial history, peanuts, chocolate, spicy and oily food consumption, wearing cosmetics, stress, and squeezing pimples (trauma) with number of acne lesion and sebum level (p < 0.05).

Effect of Doxycycline in Autologous Serum to Improvement Clinical Manifestation of Acne Vulgaris

“…[10] The study by Edison et al (2016) on the use of autologous serum containing high levels of estradiol treatment AV results occurred obtain clinical improvement and reduction in sebum after topical autologous serum. [25] Research conducted by Ilham 2011 using rats were divided into 5 groups were treated using eye drops autologous serum with various concentrations has an antibacterial effect because it contains some agents antibacterial include immunoglobulins, lysozyme and complement causes lysis of the bacteria and provide bacteriostatic, whereas anti-inflammatory effect of autologous serum based on the factors epiteliotrofik (epidermal growth factor, transforming growth factor B, fibrinogen and vitamin A) and neutrophilic (substance P, Nerve growth factor) which contain in the serum. [26] This study using autologous serum containing doxycycline in the menstrual phase to assess changes on patients with acne vulgaris, limitation of this study has no control.…”