Effects and Mechanism of Bufei Yishen Formula in a Rat Chronic Obstructive Pulmonary Disease Model

Li, Jiansheng; Yang, Liping; Yao, Qing; Li, Ya; Tian, Yange; Li, Suyun; Jiang, Shaojun; Wang, Ying; Li, Xinmin; Guo, Zheng

doi:10.1155/2014/381976

Cited by 10 publications

(13 citation statements)

References 25 publications

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“…All herbs were decocted with water, steam sterilized, and brought to a final concentration of 0.6 g/mL. BYF has been described in a previous study [ 13 ] and a Chinese patent (number 2011101175781).…”

Section: Methodsmentioning

confidence: 99%

“…We previously showed that a traditional Chinese medicine (TCM), Bufei Yishen Formula (BYF), had a curative effect on COPD, and the molecular mechanisms of its effect on COPD were investigated using transcriptomics (microarray analysis). We found that BYF treatment could significantly improve pulmonary function and lung tissue pathology of COPD rats by reducing the expression level of inflammation-related interleukins [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Such an approach is predicted to accelerate pharmacological research, such as drug target screening and validation, mechanistic research, and medicinal evaluations [ 14 – 17 ]. A modified COPD rat model was established, as described in our previous study [ 13 ] and by Chen et al [ 18 ], and three experimental groups (COPD, BYF, and controls) were used to compare pulmonary function and pathology. High performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC QTOF/MS) was used to identify differential metabolites (DMs) between the three experimental groups.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS

Yang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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As a traditional Chinese medicine, Bufei Yishen Formula (BYF) is widely used in China as an effective treatment for chronic obstructive pulmonary disease (COPD). Because of the component complexity and multiple activities of Chinese herbs, the mechanism whereby BYF affects COPD is not yet fully understood. Herein, pulmonary function experiments and histomorphological assessments were used to evaluate the curative effect of BYF, which showed that BYF had an effect on COPD. Additionally, a high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC QTOF/MS) metabonomics method was used to analyze the mechanism of the actions of BYF on rats with COPD induced by a combination of bacteria and smoking. Partial least squares discriminate analysis (PLS-DA) was used to screen biomarkers related to BYF treatment. Candidate biomarkers were selected and pathways analysis of these metabolites showed that three types of metabolic pathways (unsaturated fatty acid metabolism-related pathways, phenylalanine metabolism-related pathways, and phospholipid metabolism-related pathways) were associated with BYF treatment. Importantly, arachidonic acid and related metabolic pathways might be useful targets for novel COPD therapies.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS

Yang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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“…Previous research has confirmed that such granules could also improve COPD patient self-efficacy and satisfaction [30]. The dramatic and long-term effects of BY on decreasing inflammatory responses, reducing pulmonary pathological impairment, and airway remodeling have been shown in a COPD rat model [31][32][33][34].…”

Section: Introductionmentioning

confidence: 85%

Effect of Bufei Yishen Granules Combined with Electroacupuncture in Rats with Chronic Obstructive Pulmonary Disease via the Regulation of TLR-4/NF-κB Signaling

Tian

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Background. The combined therapy of Bufei Yishen granules (BY) and electroacupuncture (EA) has shown good effects clinically in treating chronic obstructive pulmonary disease (COPD). The present study aimed to observe the effects of the BY + EA combination in a COPD rat model and dissect the potential mechanisms via Toll-like receptor (TLR) 4/nuclear factor kappa B (NF-κB) signaling. Methods. The COPD rats were treated with normal saline, aminophylline, Bufei Yishen granules, electroacupuncture, or Bufei Yishen granules combined with electroacupuncture. The pulmonary function; lung tissue histology; levels of inflammatory factors; expression levels of TLR-4, inhibitor of nuclear factor kappa B (IκB), and NF-κB; and activation of NF-κB in the lung tissues were evaluated. Results. Pulmonary function was markedly decreased in the COPD rats, and the lung tissue histology of the COPD rats showed severe pathological changes. The pulmonary function and lung tissue morphology in the treatment groups (APL, BY, EA, and BY + EA) were improved. The increased levels of the inflammatory cytokines interleukin (IL)-1β and IL-6 indicated a chronic inflammatory state in the COPD rats. In the BY, EA, and BY + EA groups, the levels of IL-1β and IL-6 were decreased, especially in the BY + EA group. In addition, the mRNA and protein expression levels of TLR-4, IκB, and NF-κB were obviously downregulated in the BY and BY + EA groups; and the NF-κB p65 activation was significantly decreased in the BY, EA, and BY + EA groups. Conclusions. Bufei Yishen granules and electroacupuncture have curative effects in COPD rats, and the combination therapy of Bufei Yishen granules and electroacupuncture is superior. The TLR-4/NF-κB pathway may be involved in the potential mechanisms by which Bufei Yishen granules and electroacupuncture reduce inflammation.

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“…In addition to Western medicine, traditional Chinese herbal medicine (TCHM) has long been used for the clinical treatment of COPD (15,16) and the mechanisms underlying the effects of TCHM on COPD therapy have been widely studied in pre-clinical animal models of COPD (17,18). In this study, TCHM such as Bufei Jianpi, Bufei Yishen and Yiqi Zishen granules, and Liuweibuqi capsule have been demonstrated to effectively improve lung function, increase skeletal muscle strength, bone mass density and bone mineral content, and reduce airway inflammation through different mechanisms, including the inhibition of inflammatory cytokines, regulation of matrix metalloproteinases (MMPs) and their biological inhibitors (tissue inhibitors of MMPs), and modulation of the transforming growth factor-β1/Smad signal pathway (17)(18)(19)(20)(21)(22). Moreover, it has been shown that glucocorticoids used in combination with certain TCHMs have a marked effect in the prevention and treatment of COPD (23)(24)(25)(26)(27) or asthma (28).…”

Section: Introductionmentioning

confidence: 99%

Jinwei Tang modulates HDAC2 expression in a rat model of COPD

Wu¹,

Yang³

et al. 2018

Exp Ther Med

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Abstract. The aim of the present study was to investigate the effect of a Traditional Chinese Herbal Medicine (TCHM), named Jinwei Tang on histone deacetylase 2 (HDAC2) and its role in the regulation of corticosteroid resistance in a rat model of chronic obstructive pulmonary disease (COPD). Male Wistar rats were divided into five groups (each n=10): COPD group, established by the intratracheal instillation of lipopolysaccharide and passive smoke exposure, and control, budesonide, theophylline + budesonide and Jinwei Tang + budesonide groups. Lung function was measured, lung tissue histopathology was examined and HDAC2 expression in the lung was assessed by immunohistochemistry. In addition, protein levels of interleukin-8 (IL-8), tumor necrosis factor (TNF)-α and HDAC2 in lung homogenate were quantified by ELISA. The rat COPD model exhibited alterations of the ratio of forced expiratory volume in 0.2 sec (FEV 0.2 ) to the forced vital capacity, FEV 0.2 , dynamic compliance and airway resistance. HDAC2 expression was markedly reduced in the lung tissue of the COPD group compared with the control group, and treatment with Jinwei Tang + budesonide or theophylline + budesonide resulted in significant attenuation of the reduction of HDAC2 expression in the lungs (P<0.05). However, treatment with budesonide alone did not significantly alter HDAC2 expression. In the Jinwei Tang + budesonide and theophylline + budesonide groups, IL-8 and TNF-α expression was significantly decreased (P<0.05) and the HDAC2 level increased (P<0.05) compared with that in the COPD group.In conclusion, Jinwei Tang modulates airway inflammation and may enhance the anti-inflammatory effect of glucocorticoid through the upregulation of HADC2 expression in a rat model of COPD.

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Effects and Mechanism of Bufei Yishen Formula in a Rat Chronic Obstructive Pulmonary Disease Model

Cited by 10 publications

References 25 publications

Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS

Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS

Effect of Bufei Yishen Granules Combined with Electroacupuncture in Rats with Chronic Obstructive Pulmonary Disease via the Regulation of TLR-4/NF-κB Signaling

Jinwei Tang modulates HDAC2 expression in a rat model of COPD

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