Effects and mechanisms of basic fibroblast growth factor on the proliferation and regenerative profiles of cryopreserved dental pulp stem cells

Luo, Lihua; Zhang, Yanni; Chen, Hongyu; Hu, Fengting; Wang, Xiaoyan; Xing, Zhenjie; Albashari, Abdullkhaleg Ali; Xiao, Jian; He, Yan; Ye, Qingsong

doi:10.1111/cpr.12969

Cited by 21 publications

(17 citation statements)

References 52 publications

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“…In comparison, dental pulp stem cells (DPSCs), originated from neural crest and enclosed in a dental pulp chamber, seem to be an excellent source of stem cells. Because DPSCs can be extracted from discarded teeth, which is non‐invasive and raises no ethic concerns 7,8 . A recent animal study showed that exosomes secreted from DPSCs had stronger immuno‐modulating effects than those from the BMSCs 9 .…”

Section: Introductionmentioning

confidence: 99%

“…Because DPSCs can be extracted from discarded teeth, which is non‐invasive and raises no ethic concerns. 7 , 8 A recent animal study showed that exosomes secreted from DPSCs had stronger immuno‐modulating effects than those from the BMSCs. 9 An intravenous administration of DPSCs was proved to confer a similar functional recovery and superior reduction in infarct size following the middle cerebral artery occlusion in a rat model compared with the administration of BMSCs.…”

Section: Introductionmentioning

confidence: 99%

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Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response

Luo

et al. 2021

Cell Proliferation

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Objectives The study aimed to determine whether dental pulp stem cell‐derived exosomes (DPSC‐Exos) exert protective effects against cerebral ischaemia‐reperfusion (I/R) injury and explore its underlying mechanism. Materials and Methods Exosomes were isolated from the culture medium of human DPSC. Adult male C57BL/6 mice were subjected to 2 hours transient middle cerebral artery occlusion (tMCAO) injury followed by 2 hours reperfusion, after which singular injection of DPSC‐Exos via tail vein was administrated. Brain oedema, cerebral infarction and neurological impairment were measured on day 7 after exosomes injection. Then, oxygen‐glucose deprivation–reperfusion (OGD/R) induced BV2 cells were studied to analyse the therapeutic effects of DPSC‐Exos on I/R injury in vitro. Protein levels of TLR4, MyD88, NF‐κB p65, HMGB1, IL‐6, IL‐1β and TNF‐α were determined by western blot or enzyme‐linked immunosorbent assay. The cytoplasmic translocation of HMGB1 was detected by immunofluorescence staining. Results DPSC‐Exos alleviated brain oedema, cerebral infarction and neurological impairment in I/R mice. DPSC‐Exos inhibited the I/R‐mediated expression of TLR4, MyD88 and NF‐κB significantly. DPSC‐Exos also reduced the protein expression of IL‐6, IL‐1β and TNF‐α compared with those of the control both in vitro and in vivo. Meanwhile, DPSC‐Exos markedly decreased the HMGB1 cytoplasmic translocation induced by I/R damage. Conclusions DPSC‐Exos can ameliorate I/R‐induced cerebral injury in mice. Its anti‐inflammatory mechanism might be related with the inhibition of the HMGB1/TLR4/MyD88/NF‐κB pathway.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response

Luo

et al. 2021

Cell Proliferation

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“…In this study, ad-SVF spheroids could secrete more growth factors than ad-SVF and promote endothelial cell migration, which indicate a better recovery effect in UAB induced by PBOO. bFGF, as a member of FGFs, is demonstrated to suppress cell apoptosis and promote proliferation [35]. HGF was reported to be a potential anti-brotic factor in damaged tissues and pro-angiogenic factor, which has similar effects with VEGF-A [36,37].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder

Liu

Zhou²,

Zhao³

et al. 2021

Preprint

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Background: Underactive bladder (UAB) is a common clinical problem but related research is rarely explored. As there are currently no effective therapies, the administration of adipose stromal vascular fraction (ad-SVF) provides a new potential method to treat underactive bladder. Methods: Male Sprague-Dawley rats were induced by partial bladder outlet obstruction (PBOO) for four weeks and randomly divided into three groups: rats treated with PBS (Sham group); rats administrated with ad-SVF (ad-SVF group) and rats performed with ad-SVF spheroids (ad-SVFsp group). After four weeks, urodynamic studies were performed to evaluate bladder functions and all rats were sacrificed for further studies.Results: We observed that the bladder functions and symptoms of UAB were significantly improved in the ad-SVFsp group than that in the Sham group and ad-SVF group. Meanwhile, our data showed that ad-SVF spheroids could remarkably promote angiogenesis, suppress cell apoptosis and stimulate cell proliferation in bladder tissue than that in the other two groups. Moreover, ad-SVF spheroids increased the expression levels of bFGF, HGF and VEGF-A than ad-SVF. IVIS Spectrum small-animal in vivo imaging system revealed that ad-SVF spheroids could increase the retention rate of transplanted cells in bladder tissue. Conclusions: Ad-SVF spheroids improved functions and symptoms of bladder induced by PBOO, which contributes to promote angiogenesis, suppress cell apoptosis and stimulate cell proliferation. Ad-SVF spheroids provide a potential treatment for the future patients with UAB.

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“…The mitogenic effect of the bFGF on dental pulp cells was also supported recently by adding it in culture medium. Promising results were received in that the bFGF significantly promoted the proliferation of DPSCs through activating the ERK pathway without comprising cell stemness and pluripotency ( Luo et al, 2021 ). With no exceptions, the mitogenic potential of the bFGF on SHEDs was also found ( Sukarawan et al, 2014 ), and further study revealed that the bFGF promoted SHED colony-forming units via the FGFR/PI3K pathway ( Nowwarote et al, 2020 ).…”

Section: The Underlying Mechanisms Of Basic Fibroblast Growth Factor In Dental Pulp Repair and Regenerationmentioning

confidence: 99%

“…Taking those pieces of evidence together, the bFGF has the superiority to promote cell proliferation and control the cell cycle progression via stimulating the expression of related genes and proteins, including Ki67, cdc2, and cyclin B1. Therefore, its mitogenic effect can be put into use to amplify the cell number in order to obtain the sufficient amount of stem cells required for endodontic regenerative treatments and can also brighten the future of stem cell banking and tissue engineering ( Luo et al, 2021 ).…”

Section: The Underlying Mechanisms Of Basic Fibroblast Growth Factor In Dental Pulp Repair and Regenerationmentioning

confidence: 99%

The Regenerative Potential of bFGF in Dental Pulp Repair and Regeneration

Liu

et al. 2021

Front. Pharmacol.

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Regenerative endodontic therapy intends to induce the host’s natural wound-healing process, which can restore the vitality, immunity, and sensitivity of the inflammatory or necrotic pulp tissue destroyed by infection or trauma. Myriads of growth factors are critical in the processes of pulp repair and regeneration. Among the key regulatory factors are the fibroblast growth factors, which have turned out to be the master regulators of both organogenesis and tissue homeostasis. Fibroblast growth factors, a family composed of 22 polypeptides, have been used in tissue repair and regeneration settings, in conditions as diverse as burns, ulcers, bone-related diseases, and spinal cord injuries. Meanwhile, in dentistry, the basic fibroblast growth factor is the most frequently investigated. Thereby, the aim of this review is 2-fold: 1) foremost, to explore the underlying mechanisms of the bFGF in dental pulp repair and regeneration and 2) in addition, to shed light on the potential therapeutic strategies of the bFGF in dental pulp–related clinical applications.

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Effects and mechanisms of basic fibroblast growth factor on the proliferation and regenerative profiles of cryopreserved dental pulp stem cells

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 21 publications

References 52 publications

Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response

Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response

Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder

The Regenerative Potential of bFGF in Dental Pulp Repair and Regeneration

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