The 2.4-km run and 20-m multistage fitness test (20MSFT) are common aerobic fitness tests in law enforcement. Previous research analyzed relationships between these tests in recruits, and indicated limited transferability due to disparate demands (direction changes, running intensity). However, the correlations between 2.4-km run and 20MSFT in recruits postacademy training may be different, where recruits should be fitter and able to complete highintensity running efforts. This study documented changes in and relationships between the 2.4km run and 20MSFT in law enforcement recruits post-academy training. Retrospective analysis on six academy classes (276 males, 50 females) from one agency was conducted. The 20MSFT and 2.4-km run were completed pre-and post-academy training. Estimated V O2max was derived from 2.4-km run time and the 20MSFT shuttle number. Paired-samples t-tests ascertained whether the 2.4-km run and 20MSFT improved post-academy. Correlations and linear regression derived relationships between the 2.4-km run and 20MSFT. Recruits improved in the 2.4-km run and 20MSFT (p < 0.01). There were significant correlations between the 2.4km run and 20MSFT for all recruits (r = -0.49), males (r = -0.48), and females (r = -0.29).However, the regression equations for all recruits (r 2 = 0.24), males (r 2 = 0.23) and females (r 2 = 0.08) were low. The results suggested there were disparate aspects to the 2.4-km run and 20MSFT in law enforcement recruits post-academy. Even though aerobic fitness improved, there are likely other aspects important to the 20MSFT not developed during academy (changeof-direction ability, high-intensity running). This could have impact the between-test relationship.