Effects of a Dark Septate Fungal Endophyte on the Growth and Physiological Response of Seedlings to Drought in an Epiphytic Orchid

Liu, Na; Jacquemyn, Hans; Liu, Qiang; Shao, Shi-Cheng; Ding, Gang; Xing, Xiaoke

doi:10.3389/fmicb.2022.961172

Cited by 15 publications

(11 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The majority of OTUs of this order associating with orchids belonged to the family Herpotrichiellaceae (27 out of 33 OTUs, Table S3). Members of Chaetothyriales were occasionally detected in the rhizosphere of orchid roots (Herrera et al 2010; Oja et al 2017) and were found to enhance orchid seedling growth and drought resistance (Liu et al 2022).…”

Section: Resultsmentioning

confidence: 99%

“…Some endophytic fungi may produce a variety of bioactive compounds and promote orchids’ resistance to biotic and abiotic stresses (Ma et al 2015). Inoculation of a dark septate endophyte Exophiala strain ( Chaetothyriales ) was also found to enhance the drought resistance of Coelogyne seedlings (Liu et al 2022). These studies highlight the potential ecological significance of root endophytes and raise the question of whether they can act as mycorrhizal fungi under certain environmental conditions or in particular life stages of orchids.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Root-associated Fungi in Orchidaceae: Diversity, Phylogeny, Ecology, and Outstanding Questions

Wang

Lerou

Nuytinck

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Mycorrhizal fungi form ubiquitous symbiotic associations with almost all land plants and are of key interest to evolutionary biologists and ecologists because this ancient symbiosis was essential for the colonization of land by plants, a major turning point in the evolutionary history of the earth, and the subsequent development and functioning of the terrestrial ecosystems. Within the orchid family (Orchidaceae), plants establish unique interactions with specific orchid mycorrhizal fungi. These fungal symbionts are essential for the development of orchids as they provide carbon and soil nutrients to germinating orchid seeds and the nutritional supply continues for adult orchids to different degrees. Fueled by the development of DNA sequencing techniques, the diversity of mycorrhizal and other root-associated fungi in orchid roots has been extensively reported in evolutionary and ecophysiological studies. However, the full taxonomic range of orchid-associated fungi remains to be investigated in a broad phylogenetic framework, hampering a further understanding of the evolution and ecological adaptation of orchid mycorrhizal interactions. In this study, we used the most complete DNA dataset to date to map the phylogenetic distribution and ecological lifestyles of root-associated fungi in Orchidaceae by phylogenetic reconstructions at the fungal order level. We found that a broad taxonomic range of fungi (clustered into 1898 operational taxonomic units) resided in orchid roots, belonging to at least 150 families in 28 orders in Basidiomycota and Ascomycota. These fungi were assigned to diverse ecological lifestyles including typical orchid mycorrhizal fungi ("rhizoctonia"), ectomycorrhizal fungi, wood- or litter-decaying saprotrophic fungi, and other endophytes/pathogens/saprotrophs. This overview reveals that among the four different mycorrhizal types, the orchid mycorrhizal symbiosis probably involves the highest diversity of fungal taxa. We hope that our newly reconstructed phylogenetic framework of orchid-associated fungi and the assessment of their potential mycorrhizal status will benefit future ecological and evolutionary studies on orchid-fungal interactions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Root-associated Fungi in Orchidaceae: Diversity, Phylogeny, Ecology, and Outstanding Questions

Wang

Lerou

Nuytinck

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…other endophytic fungi, bacteria and nonmycorrhizal pelotons). For instance, Helotiaceae were abundant in the investigated orchid roots and DSEs being nonmycorrhizal fungi can also form peloton‐like structures in orchids (Liu et al ., 2022) and have been shown to affect isotope N signatures of plants (Giesemann et al ., 2020). Though unlikely, we cannot entirely exclude extraction artefacts, that is selective loss of parts of the fungal cell content, to have additionally influenced isotopic signatures found for the pelotons.…”

Section: Discussionmentioning

confidence: 99%

“…samples. Notably, Helotiaceae can serve beneficial dark septate endophytes (DSEs) for plants (Newsham, 2011), and the important ecological function of DSEs in the nutrition of orchids particularly in stressful environments is only just at the beginning of being addressed (Liu et al ., 2022).…”

Section: Discussionmentioning

confidence: 99%

Novel insights into orchid mycorrhiza functioning from stable isotope signatures of fungal pelotons

Zahn,

Söll,

Chapin

et al. 2023

New Phytologist

View full text Add to dashboard Cite

Summary Stable isotope signatures of fungal sporocarps have been instrumental in identifying carbon gains of chlorophyllous orchids from a fungal source. Yet, not all mycorrhizal fungi produce macroscopic sporocarps and frequently fungi of different taxa occur in parallel in orchid roots. To overcome this obstacle, we investigated stable isotope signatures of fungal pelotons extracted from orchid roots and compared these data to the respective orchid and reference plant tissues. Anoectochilus sandvicensis and Epipactis palustris represented specialized or unspecialized rhizoctonia‐associated orchids. Epipactis atrorubens and Epipactis leptochila are orchids considered ectomycorrhiza‐associated with different preferences for Basidio‐ and Ascomycota. 13C enrichment of rhizoctonia pelotons was minor compared with plant tissues and significantly lower than enrichments of pelotons from ectomycorrhizal Epipactis species. 15N values of pelotons from E. leptochila and E. atrorubens showed similar patterns as known for respective sporocarps of ectomycorrhizal Ascomycota and Basidiomycota, however, with an offset towards lower 15N enrichments and nitrogen concentrations. Our results suggest an explicit fungal nutrition source of orchids associated with ectomycorrhizal fungi, whereas the low 13C enrichment in rhizoctonia‐associated orchids and fungal pelotons hamper the detection of carbon gains from fungal partners. 15N isotopic pattern of orchids further suggests a selective transfer of 15N‐enriched protein‐nitrogen into orchids.

show abstract

“…Plant endosymbionts, such as dark septate endophytic (DSEs) fungi, known as root colonizers, are recognized for their role in improving plant health and resilience under stress to both biotic and abiotic factors via several pathways [8,[15][16][17][18]. DSEs belong to the Ascomycota phylum and have specialized hyphae that produce a large amount of melanin and are divided into separate segments.…”

Section: Introductionmentioning

confidence: 99%

The elusive bio-stimulatory function of Cladophialophora chaetospira SK51 to alleviate Fusarium wilt of strawberry

Harsonowati,

Triasih,

Narisawa

2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

The highly virulent Fusarium oxysporum formae speciales (f.sp.) fragaria causes Fusarium wilt and significant losses in the strawberry agroindustry. The effective control strategies for this phytopathogen have yet to be developed. We aimed to evaluate the bioefficacy of root-colonizer dark septate endophytic fungi (DSEs) in controlling Fusarium wilt in simultaneous with biostimulation activities. Three out of nineteen tested isolates were selected because of their superior bioefficacy in regulating disease suppression and growth promotion. Notably, the highest level of disease suppression was marked at 90.48%, followed by 85.71% and 61.90% with the inoculation of these three selected isolates i.e., SK51, SK47, and SK48, respectively. The fungal isolates were identified based on partial SSU, LSU, and ITS as DSE fungus Cladophialophora chaetospira strain SK51, Exophiala sp. strain SK47, and E. pisciphila strain SK48. Our findings showed that C. chaetospira SK51 exhibited significant potential in promoting plant growth and suppressing diseases. It significantly enhanced various plant growth factors, such as the total plant biomass, the content of chlorophyll (SPAD), the formation of flower buds, and the development of fruits compared to both non-inoculated control and the other two DSE-inoculated plants. Our study provides new findings and perspectives on the use of the DSE, particularly C. chaetospira SK51 as a bioprotectant and biostimulant for enhancing the development, flower bud formation, and fruit development in strawberry plants.

show abstract

Effects of a Dark Septate Fungal Endophyte on the Growth and Physiological Response of Seedlings to Drought in an Epiphytic Orchid

Cited by 15 publications

References 55 publications

Root-associated Fungi in Orchidaceae: Diversity, Phylogeny, Ecology, and Outstanding Questions

Root-associated Fungi in Orchidaceae: Diversity, Phylogeny, Ecology, and Outstanding Questions

Novel insights into orchid mycorrhiza functioning from stable isotope signatures of fungal pelotons

The elusive bio-stimulatory function of Cladophialophora chaetospira SK51 to alleviate Fusarium wilt of strawberry

Contact Info

Product

Resources

About