Effects of a single introduction of pamidronate on intact bone under the conditions of the experimental model of the treatment of tuberculosis ostitis

Abstract

BACKGROUND: Information about the features of intact bone tissue of the contralateral segment in the treatment of tuberculous osteitis is absent in the literature.
 AIM: Study of the features of intact bone tissue in the conditions of complex treatment of experimental tuberculous osteitis.
 MATERIALS AND METHODS: On an experimental model of tuberculous osteitis of the condyle of the right femur (Mycobacterium tuberculosis H37Rv) in 21 rabbits, the morphometric characteristics of the contralateral femurs, representatively selected from 4 groups, were studied: group (control) who did not receive either surgical or medical treatment; groups 24 radical surgical removal of the focus, supplemented: No. 2 antituberculosis therapy, group 3 antituberculosis therapy and a single injection of bisphosphonates (pamidronate), group 4 a single injection of bisphosphonates. In autopsy material studied: the area of bone tissue, the thickness of bone beams, the number of osteoblasts, osteocytes, osteoclasts and the proportion of their active forms.
 RESULTS: The following features were revealed in intact samples of different groups: group 1 the minimum area of bone tissue and the thickness of the beams, the maximum number of osteoblasts, including active ones, the average number of osteocytes; groups 2 and 3 average values of the area and thickness of bone beams, the average number of osteoblasts, the pool of osteocytes is represented mainly by inactive cells; groups 3 and 4 the minimum value of active osteoblasts; group 4 the maximum thickness of the bone beams, a greater total number of osteoblasts and osteocytes. No osteoclasts were found in any group.
 CONCLUSIONS: Different ratios of bone tissue area, thickness of bone beams, and osteoforming cellular elements in animals receiving different treatment indicate both the systemic effect of a specific process and the drug effect of anti-tuberculosis drugs and bisphosphonates on uninfected bones.