Effects of a toxin isolated from Tityus bahiensis scorpion venom on the hippocampus of rats

Ossanai, Luciene ToshieTakeishi; Lourenço, Geane Antiques; Nencioni, Ana Leonor Abrahão; Ivo, Lebrun; Yamanouye, Norma; Dorce, Valquíria Abrão Coronado

doi:10.1016/j.lfs.2012.06.029

Cited by 14 publications

(14 citation statements)

References 35 publications

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“…This might have resulted from an “all-or-none” effect, a phenomenon commonly occurring with scorpion venoms in which the effect may happen in a very strong fashion or not at all. Although this phenomenon has already been observed in several experiments in our laboratory and in clinical cases of envenoming, it lacks a clear explanation [22,40]. …”

Section: Discussionmentioning

confidence: 94%

Mild reproductive effects of the Tityus bahiensis scorpion venom in rats

Dorce

Nencioni

2014

J Venom Anim Toxins incl Trop Dis

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BackgroundScorpion envenoming is a public health problem in Brazil, where Tityus serrulatus and T. bahiensis are considered the most dangerous scorpions. They are well adapted to urbanized environments, and there is an increasing probability of human exposure to these venoms, including during pregnancy. Not much is known about the effects of prenatal exposure to the venom, and no information is available to aid in the rational treatment of victims stung during pregnancy. Thus, this study aimed to investigate whether venom from the scorpion T. bahiensis administered once to pregnant female rats at a dose that causes a moderate envenomation may lead to deleterious effects on the reproductive performance of the dams and on the development of their offspring. This is the first work demonstrating that T. bahiensis venom, when administered experimentally to rats, alters maternal reproductive performance and the morphological development of fetuses. The venom was given to dams on the 5th (GD5) or on the 10th (GD10) gestational day. After laparotomy, on GD21, fetuses and placentas were counted, weighed and externally analyzed. The corpora lutea were counted. The sex and vitality of fetuses were evaluated, and each litter was then randomly divided for visceral or skeletal analyses. Data were analyzed by ANOVA followed by the Tukey-Kramer test and Fisher’s exact test. The significance level for all tests was set at p < 0.05.ResultsGD5 group presented an increased number of pre-implantation losses. Weight gains in fetuses and placentas were observed in the GD5 and GD10 groups. Weights of the heart and lungs were elevated in GD5 and GD10 and liver weight in GD10.ConclusionsModerate envenomation by T. bahiensis scorpion venom alters maternal reproductive performance and fetal development. However, these are preliminary results whose causes should be investigated more carefully in future studies.

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Section: Discussionmentioning

confidence: 94%

Mild reproductive effects of the Tityus bahiensis scorpion venom in rats

Dorce

Nencioni

2014

J Venom Anim Toxins incl Trop Dis

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“…Indeed, cerebral alterations were assumed to be the consequence of neurotransmitter releases [43][44][45][46][47][48][49][50][51]. More recently, similar structural damage in brain tissue (i.e., edema, hemorrhage, neuronal darkness) were evoked by a small scorpion toxin called Kaliotoxin isolated from Aah [52].…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of the Androctonus Australis Hector Nanobody Nbf12-10 Antivenom to Neutralize Significantly the Toxic Effect and Tissue Damage Provoked by Fraction of Androctonus mauretanicus (Morocco) Scorpion Venom

R¹

2015

Biochem Pharmacol (Los Angel)

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Scorpion stings are life threatening in large parts of the world. Toxins from scorpion venom are responsible for severe metabolic and tissue disruption and immunotherapy is the only specific treatment able to neutralize the toxic effects of scorpion venom. The Androctonus mauretanicus (Am) is the most dangerous scorpion in Morocco, whereas in Tunisia Androctonus australis hector (Aah) is causing most casualties. In this work, we investigated the potential of NbF12-10, a new immunotherapeutic concept based on anti-toxin Nanobodies (Nbs) to neutralize Am scorpion venom. We first explored the immune cross-reactivity between Am and Aah scorpions venoms using anti-AahI and anti-AahII polyclonal, NbAahI'F12 (anti-AahI'), monospecific NbAahII10 (anti-AahII) monospecific and bispecific NbF12-10 (anti-AahI'/antiAahII) monoclonal antibodies and subsequently we study the histological damages observed after envenomation with the F3 toxic fraction of Am scorpion venom by intra-cerebroventricular (i.c.v) injection and the capacity of NbF12-10 to reduce tissue damage induced by F3 fraction after i.c.v administration of F3:NbF12-10 mixture, in mice. Results showed significant para-specific activity of anti-Aah polyclonal and monoclonal antibodies towards Am venom fractions. Histological investigations revealed severe tissue damage in brain, lung and liver after i.c.v. administration of F3 fraction. The NbF12-10 pre-mixed with F3 fraction showed an efficient neutralizing capacity against lethal effect of this toxic fraction. Moreover, in vitro pre-incubation of F3 with NbF12-10 at 8-fold molar excess led to significantly reduced tissue damage. Further, NbF12-10 displays a noteworthy potential to neutralize Am toxins and to rescue 50% of envenomed mice from dying. This study provides first evidence that NbF12-10 nano-therapeutic has promising prospective to treat scorpion envenoming in the Maghreb area.

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“…Despite no more than 10 toxins having already been isolated from this venom [20][21][22][23], their mechanisms of action remain poorly explored [12,24,25].…”

Section: Introductionmentioning

confidence: 99%

Tb1, a Neurotoxin from Tityus bahiensis Scorpion Venom, Induces Epileptic Seizures by Increasing Glutamate Release

Beraldo-Neto

Freitas

Pimenta

et al. 2020

Toxins

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Here, we report the neurotoxic effects aroused by the intracerebral injection (in rats) of Tb1, which is a neurotoxin isolated from Tityus bahiensis scorpion venom. Biochemical analyses have demonstrated that this toxin is similar to the gamma toxin from T. serrulatus, which is a β-scorpion toxin that acts on sodium channels, causing the activation process to occur at more hyperpolarized membrane voltages. Male Wistar rats were stereotaxically implanted with intrahippocampal electrodes and cannulas for electroencephalographic recording and the evaluation of amino acid neurotransmitters levels. Treated animals displayed behavioral and electroencephalographic alterations similar to epileptiform activities, such as myoclonus, wet dog shakes, convulsion, strong discharges, neuronal loss, and increased intracerebral levels of glutamate. Scorpion toxins are important pharmacological tools that are widely employed in ion channel dysregulation studies. The current work contributes to the understanding of channelopathies, particularly epilepsy, which may originate, among other events, from dysfunctional sodium channels, which are the main target of the Tb1 toxin.

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Effects of a toxin isolated from Tityus bahiensis scorpion venom on the hippocampus of rats

Cited by 14 publications

References 35 publications

Mild reproductive effects of the Tityus bahiensis scorpion venom in rats

Mild reproductive effects of the Tityus bahiensis scorpion venom in rats

Effectiveness of the Androctonus Australis Hector Nanobody Nbf12-10 Antivenom to Neutralize Significantly the Toxic Effect and Tissue Damage Provoked by Fraction of Androctonus mauretanicus (Morocco) Scorpion Venom

Tb1, a Neurotoxin from Tityus bahiensis Scorpion Venom, Induces Epileptic Seizures by Increasing Glutamate Release

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