Effects of adding luteinizing hormone to a medium containing follicle stimulating hormone on progesterone‐induced differentiation of cumulus cells during meiotic resumption of porcine oocytes

Abstract

In the present study, we investigated the effects of adding luteinizing hormone (LH) to a medium containing follicle stimulating hormone (FSH) on the shift in expression of progesterone receptor (PR) isoforms (PR-A and PR-B) and the roles in function of cumulus cells of cumulus-oocyte complexes (COC). The level of PR-B mRNA in cumulus cells was up-regulated by FSH during the first 16-h cultivation but the level was significantly decreased at 20 h. The decrease of PR-B mRNA was accelerated when COC were cultured with FSH and LH. Still, a high level of total PR mRNA was maintained in cumulus cells cultured with or without the addition of LH up to 20 h, suggesting that the expression of PR isoforms was shifted from PR-B to PR-A in cumulus cells. The reduction of PR-B was also induced by addition of progesterone to FSH-containing medium. The addition of LH or progesterone to FSH-containing medium stimulated cumulus expansion of COC as compared with that of COC cultured with FSH. In the expanded COC, ADAMTS-1 which is expressed in granulosa cells and cumulus cells in rodent follicles through LH-induced progesterone-and PR-dependent pathway, was more accumulated within the COC matrix. These results suggest that the addition of LH or progesterone to FSH-containing medium is required for the differentiation of cumulus cells, such as cumulus expansion, mediated by the shift from PR-B to PR-A in them.

Proteomic Exploration of Porcine Oocytes During Meiotic Maturation in vitro Using an Accurate TMT-Based Quantitative Approach

“…It is established that the increase in progesterone induced by a luteinizing hormone is associated with meiotic resumption of porcine oocytes (57). As we expected, the KEGG pathways analysis showed that a total of 8 over-expressed proteins were involved in the "progesterone-mediated oocyte maturation" pathway, indicating their function in regulation of oocyte maturation.…”

Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro

“…By contrast, in the pig, either a progesterone synthesis inhibitor or PGR antagonist potently suppressed the cumulus cell functions (Shimada et al 2004a(Shimada et al , 2004b, the meiotic resumption , and the developmental competence of oocytes (Shimada et al 2004b). Moreover, in pig follicles, the PGR is expressed in both cumulus and granulosa cells when cultured with FSH alone (Shimada et al 2004c) or recovered from eCG-primed gilts (Slomaczynska et al 2000). These observations suggest that, in the pig, progesterone as well as estrogen impacts functional changes in cumulus and granulosa cells that are critical for the acquisition of responses to the ovulatory LH signal.…”

The diagnostic value of transvaginal color Doppler ultrasonography plus serum β-HCG dynamic monitoring in intrauterine residue after medical abortion

“…These hormones can modulate genes and metabolism levels through neurotransmitters in the brain, foster the sensitivity and activity of the central nervous system, and ultimately promote smoothness of pregnancy, so the serum P and FSH levels in pregnant women are usually highly expressed. [ 24 – 27 ] According to pertinent investigation, after medical abortion, pregnant woman’ body has undergone major changes physiologically, the central nervous system, gene and metabolic levels have decreased sensibly, and the serum estrogen and progesterone levels have also minified accordingly. [ 28 ] Honkanenet al found that the decline in β-HCG after a medically induced abortion is inversely correlated with the time taken to abort, Which means the absolute and the relative values of β-HCG as well as the ET after medical abortion were higher in women who turned out to be late failures than in successfully treated women.…”