Effects of adiponectin on random pattern skin flap survival in rats

Tu, Qiming; Liu, Shaodong; Chen, Tingxiang; Li, Zhijie; Lin, Dingsheng

doi:10.1016/j.intimp.2019.105875

Cited by 9 publications

(8 citation statements)

References 28 publications

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“…In their study, they preferred the McFarlane flap, which is easy to harvest and less time-consuming. 12 More complex flap models were also defined in the previous studies, including composite flaps. Nasir et al 13 epigastric, superficial circumflex iliac, and deep circumflex iliac vessels, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Superior Epididymal Artery-Based Paraepididymal Adipofascial Flap

et al. 2023

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Background Flap surgery is widely performed in reconstructive surgery. Experimental research is vital to improve flap viability. However, the number of flap models for animals is still limited. In this study, we define a new adipofascial flap in rats that can be used to investigate pedicled flap and/or adipofascial flap physiology. Methods Eight Wistar male rats were used. Under deep anesthesia, paraepididymal adipofascial flaps were harvested. Flap perfusion was assessed using a near-infrared fluorescence imaging system. The length of the flap and the diameter of the flap pedicle were measured. Results All animals (n = 8) had sufficient sizes of paraepididymal fat pad, and no animals were lost. The only postoperative complication was testicular hematoma, which was observed in 2 animals. The maximum length of the harvested paraepididymal adipofascial flap was 9.7 cm with a mean of 6.6 cm. The maximum width of the flap was 3.3 cm with a mean of 2.6 cm. The mean pedicle diameter of the paraepididymal adipofascial flap was 1.1 mm. Near-infrared fluorescence imaging revealed adequate perfusion in all flaps. Conclusions The number of reported adipofascial flap models in animals is low, and they are mostly limited to flaps based on epigastric vessels. Superior epididymal artery-based paraepididymal adipofascial flap can be used as a pedicled flap model for studies focusing on adipofascial and/or pedicled flap physiology. Uncomplicated surgical technique and short operative time make this flap a valuable alternative to other flap models.

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Section: Discussionmentioning

confidence: 99%

“…For instance, Tu et al 12 designed the McFarlane flap on 36 rats to show that adiponectin increases flap viability. In their study, they preferred the McFarlane flap, which is easy to harvest and less time-consuming 12 . More complex flap models were also defined in the previous studies, including composite flaps.…”

Section: Discussionmentioning

confidence: 99%

Superior Epididymal Artery-Based Paraepididymal Adipofascial Flap

et al. 2023

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“…Flap transplantation technology is an important technical mean in the field of orthopedics (Picard-Ami et al 1991;Tu et al 2019;Varghese et al 2019). It is widely used in other special fields such as orthopedics, microsurgery, oral and maxillofacial surgery, ophthalmology, etc.…”

Section: Introductionmentioning

confidence: 99%

“…It is widely used in other special fields such as orthopedics, microsurgery, oral and maxillofacial surgery, ophthalmology, etc. (Picard-Ami et al 1991;Tu et al 2019;Varghese et al 2019). However, flap transplanted often sustains ischemia and hypoxia for a period of time, and then reperfusion of blood after ischemia, leading to an inevitably damage to the flap tissue and even its survival (Picard-Ami et al 1991;Tu et al 2019;Varghese et al 2019;Liu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…(Picard-Ami et al 1991;Tu et al 2019;Varghese et al 2019). However, flap transplanted often sustains ischemia and hypoxia for a period of time, and then reperfusion of blood after ischemia, leading to an inevitably damage to the flap tissue and even its survival (Picard-Ami et al 1991;Tu et al 2019;Varghese et al 2019;Liu et al 2019). Nowadays, it is believed that the production of oxygen free radicals, calcium overload, energy metabolism disorders, vascular endothelial cell dysfunction, cytokine or growth factor production, necrosis are the critical mechanisms in the I/R injury to skin flap (Chen et al 2018;Zhang et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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D-allose alleviates ischemia/reperfusion (I/R) injury in skin flap via MKP-1

Hou

Zhang

2020

Mol Med

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Background: D-allose was promising in the protection of ischemia/reperfusion (I/R) injury. We intended to investigate the function of D-allose in skin flap of rat followed by the injury of I/R and whether ERK signal pathway was involved in. Methods: The back flap of Wistar rats was picked up with a vascular bundle of the lateral chest wall. I/R model was made by the venous clamp for 6 h. Rats received D-allose and PD-98059, the inhibitor of ERK1/2, 30 min before modeling. Morphology of tissue was observed by HE staining. Nitric oxide (NO), myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD) levels in skin flap were determined by ELISA kits. mRNA and protein levels were determined by qPCR and Western blot respectively. Results: D-allose alleviated the condition of pathological changes and raised the survival rate of skin flap injured by I/R. Moreover, D-allose suppressed NO, MPO and MDA while elevated SOD levels during I/R status. Furthermore, Dallose decreased MCP-1, TNF-α, IL-1β and IL-6 levels in skin flap injured by I/R. In addition, D-allose inhibited MKP-1 expression and activated ERK1/2 pathway in skin flap injured by I/R. PD-98059 partially counteracted D-allose effects on I/R injury. Conclusions: D-allose exerted its protective function via inhibiting MKP-1expression and further activated ERK1/2 pathway to suppress the progress of oxidative stress, inflammation and necrosis, contributing to the survival of skin flap injured by I/R. Thus, D-allose was promising in the transplantation of skin flap.

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