Effects of administering tocopherol after irradiation on survival and proliferation of murine lymphocytes

Roy, R. M.; Petrella, Marco; Shateri, Hossein

doi:10.1016/0163-7258(88)90089-7

Cited by 18 publications

(13 citation statements)

References 2 publications

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“…A similar effect was observed in an earlier study in which an increase in the drug dose from 200 mg to 800 mg resulted in a significant elevation of the P/N ratio (13). An elevated mitogenic response has been reported in in vitro irradiated splenic lymphocytes treated with vitamin E (29).…”

Section: Discussionsupporting

confidence: 70%

In VivoPostirradiation Protection by a Vitamin E Analog, α-TMG

Satyamitra

Devi

Murase³

et al. 2003

Radiation Research

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The water-soluble vitamin E derivative alpha-TMG is an excellent radical scavenger. A dose of 600 mg/kg TMG significantly reduced radiation clastogenicity in mouse bone marrow when administered after irradiation. The present study was aimed at investigating the radioprotective effect of postirradiation treatment with alpha-TMG against a range of whole-body lethal (8.5-12 Gy) and sublethal (1-5 Gy) doses of radiation in adult Swiss albino mice. Protection against lethal irradiation was evaluated from 30-day mouse survival and against sublethal doses was assessed from micronuclei and chromosomal aberrations in the bone marrow 24 h after irradiation. An intraperitoneal injection of 600 mg/kg TMG within 10 min of lethal irradiation increased survival, giving a dose modification factor (DMF) of 1.09. TMG at doses of 400 mg/kg and 600 mg/kg significantly reduced the percentage of aberrant metaphases, the different types of aberrations, and the number of micronucleated erythrocytes. DMFs of 1.22 and 1.48 for percentage aberrant metaphases and 1.6 and 1.98 for micronuclei were obtained for 400 mg/kg and 600 mg/kg TMG, respectively. No drug toxicity was observed at these doses. The effectiveness of TMG when administered postirradiation suggests its possible utility for protection against unplanned radiation exposures.

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Section: Discussionsupporting

confidence: 70%

In VivoPostirradiation Protection by a Vitamin E Analog, α-TMG

Satyamitra

Devi

Murase³

et al. 2003

Radiation Research

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“…The radiomitigative potential of AT was first observed in 1978 when an aqueous preparation was used intraperitoneally [ 62 ]. AT's radiomitigative effect in mice was linked to enhanced cell-mediated immunity [ 63 ].…”

Section: Introductionmentioning

confidence: 99%

Vitamin E: tocopherols and tocotrienols as potential radiation countermeasures

Singh¹,

Beattie²,

Seed

2013

Journal of Radiation Research

111

101

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Despite the potential devastating health consequences of intense total-body irradiation, and the decades of research, there still remains a dearth of safe and effective radiation countermeasures for emergency, radiological/nuclear contingencies that have been fully approved and sanctioned for use by the US FDA. Vitamin E is a well-known antioxidant, effective in scavenging free radicals generated by radiation exposure. Vitamin E analogs, collectively known as tocols, have been subject to active investigation for a long time as radioprotectors in patients undergoing radiotherapy and in the context of possible radiation accidents or terrorism scenarios. Eight major isoforms comprise the tocol group: four tocopherols and four tocotrienols. A number of these agents and their derivatives are being investigated actively as radiation countermeasures using animal models, and several appear promising. Although the tocols are well recognized as potent antioxidants and are generally thought to mediate radioprotection through ‘free radical quenching’, recent studies have suggested several alternative mechanisms: most notably, an ‘indirect effect’ of tocols in eliciting specific species of radioprotective growth factors/cytokines such as granulocyte colony-stimulating factor (G-CSF). The radioprotective efficacy of at least two tocols has been abrogated using a neutralizing antibody of G-CSF. Based on encouraging results of radioprotective efficacy, laboratory testing of γ-tocotrienol has moved from a small rodent model to a large nonhuman primate model for preclinical evaluation. In this brief review we identify and discuss selected tocols and their derivatives currently under development as radiation countermeasures, and attempt to describe in some detail their in vivo efficacy.

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“…Cellular viability was determined using ethidium bromide and acridine orange (27) in order to adjust the cell concentration according to the viable cells number. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test was used to evaluate the radiosensitivity according to cellular viability (21).…”

Section: Methodsmentioning

confidence: 99%

Efecto de la irradiación-γ in vitro sobre la respuesta mitogénica de linfocitos de ratón

2008

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The objective of this study was to analyze the proliferative response of BALB/c mice lymphocytes after in vitro irradiation (0.05 to 6 Gy). The capability of irradiated lymphocytes for proliferating without any stimulation and after activation with specific T and B cell mitogens has been evaluated. The results show that ionizing radiation significantly inhibits spontaneous cellular proliferation and that induced by mitogens and that variations in the degree of inhibition are found depending on the inducing proliferation mitogens and the dosage applied. The conclusion drawn is that different lymphocyte populations have different radiosensitivities, being B cells more sensitive to ionizing irradiation than T cells. Besides, the effects of gamma-irradiation vary according to the different subpopulations of T cells or, alternatively, to different T-dependent activation mechanisms.

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Effects of administering tocopherol after irradiation on survival and proliferation of murine lymphocytes

Cited by 18 publications

References 2 publications

In VivoPostirradiation Protection by a Vitamin E Analog, α-TMG

In VivoPostirradiation Protection by a Vitamin E Analog, α-TMG

Vitamin E: tocopherols and tocotrienols as potential radiation countermeasures

Efecto de la irradiación-γ in vitro sobre la respuesta mitogénica de linfocitos de ratón

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