Effects of albusin B (a bacteriocin) of Ruminococcus albus 7 expressed by yeast on growth performance and intestinal absorption of broiler chickens-its potential role as an alternative to feed antibiotics

Wang, Han‐Tsung; Yu, Chung Huang; Hsieh, Ya-Hui; Chen, Shiau-Wei; Chen, Bao-Ji; Chen, Ching-Yi

doi:10.1002/jsfa.4463

Cited by 30 publications

(42 citation statements)

References 35 publications

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“…This suggests that multi-strain lactobacilli mixture composition might be a major concern in probiotic supplementation. The BA product applied in this study was a yeast with bacteriocin; it has been shown to be a broiler growth promoter owing to its ability to enhance the lactic acid bacteria growth in digestive tract and antibiotic activity [8]. In a broiler model, dietary supplementation with 0.15% to 0.3% S. cerevisiae resulted in a growth performance equal to that observed with antibiotic supplementation, and broiler chickens with yeast supplementation had better growth and carcass weight than control broilers [17].…”

Section: Discussionmentioning

confidence: 99%

“…Cloning of the structural gene and plasmid construction for expression in S. cerevisiae and mass production of bacteriocin-expressing yeast were conducted as described by Wang et al [8]. The alb B gene (NCBI: AF469209) of albusin B from R. albus 7 was applied for expression in S. cerevisiae DBY 747 as a bacteriocin source.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously established Saccharomyces cerevisiae as a stable and safe expression system for the mass production of albusin B [8]. A previous study also suggested that yeast with albusin B inhibits pathogenic bacteria, increases intestinal absorption of protein and glucose, and improves gut barrier function.…”

Section: Introductionmentioning

confidence: 99%

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Effect of supplementation of yeast with bacteriocin and Lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens

Chen

Wang

2016

Asian-Australas J Anim Sci

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ObjectiveThe aim of the present study was to investigate the effect of yeast with bacteriocin and Lactobacillus cultures (mixture of Lactobacillus agilis BCRC 10436 and Lactobacillus reuteri BCRC 17476) supplements, alone or in combination, on broiler chicken performance.MethodsA total of 300, 1-d-old healthy broiler chickens were randomly divided into five treatment groups: i) basal diet (control), ii) basal diet+0.25% yeast (Saccharomyces cerevisiae) (YC), iii) basal diet+0.25% yeast with bacteriocin (BA), iv) basal diet+Lactobacillus cultures (LAB), and v) basal diet +0.25% yeast with bacteriocin+Lactobacillus cultures (BA+LAB). Growth performance, cecal microbiota, cecal fermentation products, and blood biochemistry parameters were determined when chickens were 21 and 35 d old.ResultsThe supplementation of YC, BA, and BA+LAB resulted in a significantly better feed conversion rate (FCR) than that of the control group during 1 to 21 d (p<0.05). The LAB supplementation had a significant effect on the presence of Lactobacillus in the ceca at 35 d. None of the supplements had an effect on relative numbers of L. agilis and L. reuter at 21 d, but the BA supplementation resulted in the decrease of both Lactobacillus strains at 35 d. The BA+LAB supplementation resulted in higher short chain fatty acid (SCFA) in the ceca, but LAB supplementation significantly decreased the SCFA at 35 d (p<0.05). All treatments tended to decrease ammonia concentration in the ceca at 21 d, especially in the LAB treatment group. The BA supplementation alone decreased the triacylglycerol (TG) concentration significantly at 21 d (p<0.05), but the synergistic effect of BA and LAB supplementation was required to reduce the TG concentration at 35 d. The YC supplementation tended to increase the plasma cholesterol at 21 d and 35 d. However, the BA supplementation significantly decreased the cholesterol and low density lipoprotein cholesterol level at 35 d. In conclusion, the BA+LAB supplementation was beneficial to body weight gain and FCR of broiler chickens.ConclusionThe effect of BA and LAB supplementation may be a result of the growth of lactic acid bacteria enhancement and physiological characterization of bacteriocin, and it suggests that the BA and LAB supplementation level or Lactobacillus strain selection should be integrated in future supplementation designs.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effect of supplementation of yeast with bacteriocin and Lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens

Chen

Wang

2016

Asian-Australas J Anim Sci

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“…Although the factors affecting the production of albusin B by R. albus have been studied, much less regarding the mechanisms of action of these bacteriolysins is understood. Nonetheless, when recombinant Saccharomyces cerevisiae DBY 747 expressing albusin B of R. albus 7 was fed (at 2.5 g kg of body weight Ϫ1 ) to broiler chickens, an improvement of growth performance was observed, suggesting that ruminal bacteriocins could have a role as potential alternatives to the growth promoters used in livestock production (55).…”

Section: Discussionmentioning

confidence: 99%

Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes

Azevedo

Bento

Ruiz

et al. 2015

Appl Environ Microbiol

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b Some species of ruminal bacteria are known to produce antimicrobial peptides, but the screening procedures have mostly been based on in vitro assays using standardized methods. Recent sequencing efforts have made available the genome sequences of hundreds of ruminal microorganisms. In this work, we performed genome mining of the complete and partial genome sequences of 224 ruminal bacteria and 5 ruminal archaea to determine the distribution and diversity of bacteriocin gene clusters. A total of 46 bacteriocin gene clusters were identified in 33 strains of ruminal bacteria. Twenty gene clusters were related to lanthipeptide biosynthesis, while 11 gene clusters were associated with sactipeptide production, 7 gene clusters were associated with class II bacteriocin production, and 8 gene clusters were associated with class III bacteriocin production. The frequency of strains whose genomes encode putative antimicrobial peptide precursors was 14.4%. Clusters related to the production of sactipeptides were identified for the first time among ruminal bacteria. BLAST analysis indicated that the majority of the gene clusters (88%) encoding putative lanthipeptides contained all the essential genes required for lanthipeptide biosynthesis. Most strains of Streptococcus (66.6%) harbored complete lanthipeptide gene clusters, in addition to an open reading frame encoding a putative class II bacteriocin. Albusin B-like proteins were found in 100% of the Ruminococcus albus strains screened in this study. The in silico analysis provided evidence of novel biosynthetic gene clusters in bacterial species not previously related to bacteriocin production, suggesting that the rumen microbiota represents an underexplored source of antimicrobial peptides.T he production of low-molecular-weight antimicrobial peptides is a trait widely distributed among species of bacteria and archaea (1). Although a variety of functions has been assigned to these compounds (e.g., toxins, virulence factors, bacterial hormones), the bacteriocins have mainly been investigated due to their potential as alternatives to antibiotics and food preservatives (2-4). With the rise in antibiotic resistance among commensal and pathogenic strains of bacteria, there is an urgent need to identify and develop novel therapeutic strategies for clinical applications in human and animal health (5).Bacteriocins are often defined as ribosomally synthesized antimicrobial peptides produced by bacteria or archaea (6). Bacteriocins show great diversity in their chemical structures and mechanisms of action, and at least four main groups have been proposed to classify these antimicrobial agents (7). Class I contains posttranslationally modified antimicrobial peptides, such as lanthipeptides, sactipeptides, and lasso peptides, which differ in their molecular structures, their mechanisms of action, and the enzymatic apparatuses involved in modifying the precursor peptides (7,8). Class II bacteriocins consist of antimicrobial peptides without posttranslationally modified residues and ca...

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“…Este gênero tem sido relacionado com a melhora na conversão alimentar, além da espécie Ruminococcus torques relacionada a melhora do desempenho das aves (TOROK et al, 2011;STANLEY, 2016). Estudo mostrou que a suplementação de dietas com bacteriocina produzida pelo Ruminococcus albus aumentou a absorção intestinal de nutrientes e elevou o número de Lactobacillus, diminuindo a população de bactérias patogênicas (WANG et al, 2011). Outro membro desta família é o gênero Anaerofilum, composto por bactérias anaeróbias que fermentam mono e dissacarídeos produzindo lactato, acetato e outros .…”

Section: Membros Da Família Staphylococcaceae E Ordem Bacillales Os unclassified

Diferentes estratégias do uso de sorgo para frangos de corte: desempenho e saúde intestinal

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Effects of albusin B (a bacteriocin) of Ruminococcus albus 7 expressed by yeast on growth performance and intestinal absorption of broiler chickens-its potential role as an alternative to feed antibiotics

Cited by 30 publications

References 35 publications

Effect of supplementation of yeast with bacteriocin and Lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens

Effect of supplementation of yeast with bacteriocin and Lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens

Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes

Diferentes estratégias do uso de sorgo para frangos de corte: desempenho e saúde intestinal

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