Effects of Aliphatic Aldehydes on the Growth and Patulin Production of<i>Penicillium expansum</i>in Apple Juice

Taguchi, Takumi; Kozutsumi, Daisuke; Nakamura, Ruka; Satō, Yoshio; Ishihara, Atsushi; Nakajima, Hiromitsu

doi:10.1271/bbb.120629

Cited by 11 publications

(3 citation statements)

References 34 publications

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“…1A). The growth curve of P. expansum in the apple juice observed in this experiment is quite similar to that reported by Taguchi et al [23]. We also monitored the changes in reducing sugar content in the medium during cultivation (Fig.…”

Section: Resultssupporting

confidence: 86%

Proteome Changes in Penicillium expansum Grown in a Medium Derived from Host Plant

Xia¹,

Li²,

Liu³

et al. 2017

Journal of Microbiology and Biotechnology

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causes blue mold rot, a prevalent postharvest disease of pome fruit, and is also the main producer of the patulin. However, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. In this work, a two-dimensional gel electrophoresis-based proteomic approach was applied to probe changes in 3.3703 cultivated in apple juice medium, which was used to mimic the in planta condition. The results showed that the pH value and reducing sugar content in the apple juice medium decreased whereas the patulin content increased with the growing of. A total of 28 protein spots that were up-regulated in when grown in apple juice medium were identified. Functional categorization revealed that the identified proteins were mainly related to carbohydrate metabolism, secondary metabolism, protein biosynthesis or degradation, and redox homeostasis. Remarkably, several induced proteins, including glucose dehydrogenase, galactose oxidase, and FAD-binding monooxygenase, which might be responsible for the observed medium acidification and patulin production, were also detected. Overall, the experimental results provide a comprehensive interpretation of the physiological and proteomic responses of to the host plant environment, and future functional characterization of the identified proteins will deepen our understanding of fungi-host interactions.

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Section: Resultssupporting

confidence: 86%

Proteome Changes in Penicillium expansum Grown in a Medium Derived from Host Plant

Xia¹,

Li²,

Liu³

et al. 2017

Journal of Microbiology and Biotechnology

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show abstract

“…T2H could inhibit fungal growth in broth . Considering that T2H is not water-soluble, we decided to test whether it could affect mycelial biomass in YEPD liquid medium.…”

Section: Resultsmentioning

confidence: 99%

“…graminearum in YEPD liquid medium. Similarly, T2H reduced the bacterial numbers of Pseudomonas syringae, Aeromonas sobria, and Aeromonas hydrophila in tryptic glucose yeast (TGY) broth and Penicillium expansum growth in apple juice broth. , Experimental data on contact angle and surface tension highlighted that good lipophilicity is the basis for the tight binding of T2H to the F. graminearum mycelia.…”

Section: Discussionmentioning

confidence: 98%

Green Leaf Volatile Trans-2-Hexenal Inhibits the Growth of Fusarium graminearum by Inducing Membrane Damage, ROS Accumulation, and Cell Dysfunction

Wang

Zhu

et al. 2022

J. Agric. Food Chem.

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Fusarium graminearum, the main agent of Fusarium head blight (FHB), can cause serious yield loss and secrete mycotoxins to contaminate grain. Here, the biological activity of trans-2-hexenal (T2H) against F. graminearum was determined and its mode of action (MOA) was investigated. Furthermore, surface plasmon resonance with liquid chromatography–tandem mass spectrometry (SPR-LC–MS/MS), bioinformatic analysis, and gene knockout technique were combined to identify the binding proteins of T2H in F. graminearum cells. T2H exhibited satisfactory inhibitory activity against F. graminearum in vitro. Good lipophilicity greatly enhanced the affinity of T2H to F. graminearum mycelia and further caused membrane damage. The FgTRR (thioredoxin reductase) gene negatively regulates the sensitivity of F. graminearum to T2H by reducing the generation of reactive oxygen species (ROS) induced by T2H. Two mutant strains with FgSLX1 (structure-specific endonuclease subunit) and FgCOPB (coatomer subunit β) genes knockout showed decreased sensitivity to T2H, suggesting that these two genes may be involved in the antimicrobial activity of T2H. Taken together, T2H can inhibit F. graminearum growth by multiple MOAs and can be used as a biofumigant to control the occurrence of FHB in the field.

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Inhibitory Mechanisms of Plant Volatile 1-Octanol on the Germination of Aspergillus Flavus Spores

et al. 2023

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Effects of Aliphatic Aldehydes on the Growth and Patulin Production ofPenicillium expansumin Apple Juice

Cited by 11 publications

References 34 publications

Proteome Changes in Penicillium expansum Grown in a Medium Derived from Host Plant

Proteome Changes in Penicillium expansum Grown in a Medium Derived from Host Plant

Green Leaf Volatile Trans-2-Hexenal Inhibits the Growth of Fusarium graminearum by Inducing Membrane Damage, ROS Accumulation, and Cell Dysfunction

Inhibitory Mechanisms of Plant Volatile 1-Octanol on the Germination of Aspergillus Flavus Spores

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