Effects of altering medium strength and sucrose concentration onin vitrogermination and seedling growth ofCypripedium macranthosSw.

Abstract

The genus Cypripedium, known as lady's slipper orchid, has been considered to have a considerable marketability as a potted and garden plant with beautiful flowers; however, this species is becoming endangered and, in some places extinct, due to habitat destruction and illegal collections. As such, an optimized artificial propagation system is necessary for its conservation and horticultural cultivation. For the establishment of the in vitro proliferation of the endangered Cypripedium macranthos Sw., native to Korea, the effects of medium strength and sucrose concentrations on germination, protocorm formation and seedling growth were investigated through asymbiotic seed culture. The highest germination rate and protocorm formation rate were obtained with a 1/4 MS medium; higher MS medium strengths did not generate the favorable conditions required for germination and protocorm growth. The overall growth characteristics of roots and buds developed from protocorms were best in cultures of 1/4 MS medium. On this medium containing 10 g•L-1 sucrose, both the germination rate and protocorm formation rate increased significantly. The general growth properties of seedlings developed on the medium with 10 g•L-1 sucrose were best, showing the highest bud formation rate and root number. Our results demonstrate that the 1/4 MS medium, supplemented with 10 g•L-1 sucrose, could improve in vitro germination, and facilitate the growth of seedlings developed from the protocorms of C. macranthos Sw.

In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm

“…The success of seed germination through in vitro culture is influenced by several factors, including the types of culture media [9, 10], seed maturity [11, 12], plant growth regulators [13, 14], carbohydrates [15], and organic amendments [16].…”

Armazenamento de sementes colhidas de diferentes posições do escapo floral para obtenção de plantas da bromélia imperial - Alcantarea imperialis

“…a taxa de germinação não apresentou diferença entre aquelas mantidas em meio de cultura sem sacarose ou suplementado com 20 g.L -1 (Pickens et al 2003). Já em sementes da orquídea Cypripedium macranthos Sw., a germinação foi favorecida no meio de cultura com 10 g.L -1 em comparação com aqueles em que a sacarose era ausente ou apresentavam uma concentração de 20, 30 ou 40 g.L -1 (Huh et al 2016). Para A. imperialis, o acréscimo de sacarose favoreceu a emergência das plantas apenas com o uso de sementes da base, provavelmente devido a estas estarem em seu estado de maturação fisiológica, conforme relatado anteriormente.…”

The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans

“…On the other hand, some other orchid species (Microtis parviflora, Caladenia formosa) grew better with the presence of sucrose (Huynh al. 2004, Wright et al 2009 especially those germinated asymbiotically (Huh et al 2016) so there is a need for individualised ingredients to cater for the preferences of both orchid and fungus.…”