Effects of Aluminum in the striped venus clam, Chamelea gallina

Guglielmi, Marco Vito; Semeraro, Daniela; Mentino, Donatella; Patricelli, Eliana; Mastrototaro, Francesco; Carlucci, Roberto; Mastrodonato, María; Scillitani, Giovanni

doi:10.1109/metrosea55331.2022.9950929

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…Nuclei were counterstained with Mayer's hematoxylin and mounted with DPX for observation. Details about histochemical techniques can be found elsewhere Guglielmi, Semeraro, Ricci, et al, 2023). All the chemicals were from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Classic Histochemistrymentioning

confidence: 99%

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Altered glycosylation in secreting cells of the gastric glands of aquaporin‐4‐deficient mice

Mentino,

Nicchia,

Frigeri

et al. 2024

Microscopy Res & Technique

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Aquaporins (AQPs) are important for water transport in the gastrointestinal tract. Changes in their expression and/or localization could cause in disorders and be used as therapeutic targets. Aquaporin‐4 (AQP4) is expressed predominantly on the basolateral membrane of the parietal cells in the corpus of the murine gastric glands. Although the secretion of gastric juice is not affected in AQP4‐deficient knockout, we evaluated by light microscopy whether the lack of AQP4 affects the glycopatterns of secreting gastric cells. Wild type (WT) and AQP4‐deficient knockout mice (KO) were fed a standard diet ad libitum before sacrifice. Segments of stomach corpus were collected, fixed in buffered formalin, and embedded in paraffin wax. Sections, 5‐μm thick, were analyzed by histochemical methods (Periodic acid‐Schiff, Alcian Blue pH 2.5), and binding of lectins specific to GalNAc (SBA, DBA), Gal (PNA) GlcNAc (WGA, GSAII) mannose and/or glucose (ConA), and fucose (UEA‐I, AAA, LTA). Immunohistochemical methods such as anti‐Muc6 for neck cells and anti‐ β‐ H+/K+‐ATPase for parietal cells were also performed. Compared to WT mice, in the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated, and fucosylated residues were observed; lower fucosylation resulted also in the parietal cells. The observed differences of KO in respect to WT could lead to severer pathological conditions.Research Highlights Glycopatterns in gastric glands were compared between wild type (WT) and AQP4‐deficient knockout (KO) mice by histochemical and lectin‐binding methods. In the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated and fucosylated residues were observed. In the parietal cells lower fucosylation also resulted. AQP4‐deficiency affects glycosylation and could result in altered functionality and pathological conditions.

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Section: Classic Histochemistrymentioning

confidence: 99%

“…Subsequently, they were rinsed in the same buffer and mounted in Fluoromount (Sigma). Refer to and Guglielmi, Semeraro, Ricci, et al (2023) for further details.…”

Section: Fluorescent Lectin Bindingmentioning

confidence: 99%