Effects of amphotericin B on hepatitis B virus

Abstract

We investigated the effects of amphotericin B (AmB) on the ultrastructure and biochemistry of hepatitis B virus (HBV) core component possesses an immunologically distinct hepatitis B core antigen (HBcAg) reactivity which on further disruption reveals the third HBV-associated antigen, hepatitis B e antigen (21). Also present in the sera of infected individuals are 22-nm HBsAg particles, which have been shown by biochemical analysis to contain approximately 23% lipid by dry weight in addition to protein and carbohydrates (25). Neutral lipids account for 22.3% of this fraction, with 59% of these being cholesterol and cholesterol esters (25). We predicted, therefore, that AmB would have an effect on the structural integrity of HBV particles, assuming the outer lipid membrane to be of approximately the same chemical composition as HBsAg particles. This study was therefore undertaken to determine the ultrastructural effects of AmB on HBV and the 22-nm form of HBsAg as determined by HBV deoxyribonucleic acid (DNA) polymerase activity, electron microscopy (EM), differential ultracentrifugation, and sucrose density centrifugation.MATERIALS AND METHODS HBV and HBsAg. Partially purified HBV and purified 22-nm HBsAg particles were obtained as previously described from a chimpanzee chronically infected with HBV (10, 22). Radioiodinated purified HBsAg particles were prepared as described by Skelly et al. (23). HBV-associated DNA polymerase activity was determined by the method of Howard (9)

Effect of deoxycholate, amphotericin B and fongizone on transmissible gastroenteritis coronavirus

“…However, when AmB and DOC were combined, they were found to have an effect similar to that of fongizone, These results are consistent with those observed by DeVald et al [6] and Kessler et al [10], but not with results obtained by Jordan and Seet (1978), who claimed that fongizone at a concentration of 2 fLg/ml did not affect enveloped virus infectivity. However, Kessler et al [10] claimed that AmB inactivated hepatitis B virus, when they used fongizone, a compound comprising AmB and DOC. It can be assumed that AmB alters the viral envelope by creating pores, similar to treated eukaryote cell membranes [7,8,15].…”

Novel paradigms for drug discovery: computational multitarget screening

“…Our novel protocol is extremely effective and increases success rates downstream in preclinical and clinical use with a considerable reduction in time, effort and cost expended. Table 1 Compounds with inhibitory activity against HIV-1 and other microorganisms or diseases a [55] Aspergillus fumigatus [56] Blastomyces dermatitidis [57] Candida genus [56] Cryptococcus neoformans [56] Fusarium species [58] Hepatitis B virus [59] Histoplasma capsulatum [60] Chloroquine Integrase IC 50 = 5.14 [61] Cryptococcus neoformans [62] Reverse transcriptase IC 50 >300…”

Solution NMR structure of five representative glycosylated polyene macrolide antibiotics with a sterol‐dependent antifungal activity

“…This gives to polyene macrolides therapies undesired hemolytic and nephrolytic side‐effects. Other relevant effects assigned to some polyene macrolides, such as antiviral properties against several groups of enveloped viruses [6,7] or stimulation of the immune response at lower concentrations [8,9], have been also reported. These activities make of polyene macrolides a source of lead structures for the engineering of future molecules with improved medicinal purposes.…”