Effects of an aqueous extract from<i>Ammodaucus leucotrichus</i>on calcium oxalate crystallization<i>in vitro</i>

Beghalia, Mohamed; Ghalem, Saïd; Allali, Hocine; Belouatek, Aissa; Marouf, A.

doi:10.5958/j.0975-4261.1.1.006

Cited by 4 publications

(5 citation statements)

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“…In the same year, the same research team studied in vitro the antilithiasic effect of C. humilis bark (Algeria) using the same method. C. humilis bark aqueous extract inhibited in vitro the formation of calcium oxalate crystallization only for growth phase (Beghalia et al, 2008).…”

Section: Antilithiasic Activitymentioning

confidence: 95%

“…In a study conducted by Beghalia et al (2008), the antilithiasic effect of C. humilis sheath aqueous extract from Algeria was tested on synthetic urine by simulating oxalate crystallisation at different stages, including nucleation, development, and aggregation. Using aqueous extract of C. humilis, the inhibition of calcium oxalate crystallization was observed only for growth phase.…”

Section: Antilithiasic Activitymentioning

confidence: 99%

“…These different classes of phytocompounds play a role in the pharmacological activities of C. humilis. Many researchers have reported a wide range of potential activities associated with C. humilis extracts and essential oils including antibacterial, anticholinesterase, antidiabetic, antilithiasic, anti-inflammatory, antitumoral, antioxidant, antityrosinase, and antihyperlipidemic activities (Figure 1) (Beghalia et al, 2008;Bennaceur et al, 2010;Gaamoussi et al, 2010;Hasnaoui et al, 2013;Miguel et al, 2014;Belhaoues et al, 2017;Gonçalves et al, 2018;Aicha et al, 2019;Dawood et al, 2020;Attaallah et al, 2021;Lachkar et al, 2022;Rincón-Cervera et al, 2023). However, there are scarcely those reported the toxicity effect of this plant (Lachkar et al, 2022).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Ethnomedicinal use, phytochemistry, pharmacology, and toxicology of Chamaerops humilis: A review

KHOUCHLAA

2023

Not Sci Biol

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Chamaerops humilis L. is a widespread species in the western and central Mediterranean region. It is used in traditional medicine to treat numerous diseases, particularly diabetes. Aim of the review: This review was devoted to provide valuable information on C. humilis various aspects including its botanical description, taxonomy, geographical distribution, medicinal use, phytochemistry, pharmacological properties, and toxicity. Materials and methods: We searched various scientific databases, such as Scopus, PubMed, Web of Science, SpringerLink, SciFinder, Wiley Online, and Google Scholar, to collect data on C. humilis. The presented data on C. humilis were organized according to botanical description, ethnomedicinal use, bioactive compounds, pharmacology, and toxicological investigation. Results: In traditional medicine, C. humilis was used to treat diabetes, diarrhea, gastritis, gastralgia, constipation, neuronal diseases, and anemia. The extracts and essential oil derived from C. humilis demonstrated various beneficial properties, such asantibacterial, anticholinesterase, antidiabetic, antilithiasic, anti-inflammatory, antitumoral, antioxidant, antityrosinase, and antihyperlipidemic activities. Phytochemical investigations identified several chemical classes of secondary metabolites in C. humilis essential oil and extracts including phenols, sterols, terpenoids, polysaccharides, and fatty acid. Conclusion: Based on the critical analysis of previous studies, further exploration of C. humilis and its bioactive compounds should be conducted for potential medical applications. This may involve isolating and characterizing specific compounds from C. humilis and evaluating their therapeutic potential through preclinical and clinical studies.

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Section: Antilithiasic Activitymentioning

confidence: 95%

Section: Antilithiasic Activitymentioning

confidence: 99%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Ethnomedicinal use, phytochemistry, pharmacology, and toxicology of Chamaerops humilis: A review

KHOUCHLAA

2023

Not Sci Biol

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“…However, urinary crystallite can agglomerate quickly in a short time. Thus, its size could increase rapidly in a short time 9 . Therefore, the ability of affecting the formation of urinary stone in a descending order is as follows: agglomeration > nucleation > growth.…”

Section: Effect Of Carboxylates On Reaction Order and Reaction Rate Cmentioning

confidence: 99%

“…Therefore, artificial urine is widely used. For example, Beghalia et al 9 studied the effect of ajuga and atriplex, which are two types of herbal medicine, on CaC2O4 crystallization in Asian Journal of Chemistry;Vol. 26, No.…”

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confidence: 99%

Effect of Unitary, Binary and Ternary Carboxylates on Crystallization Kinetics of Calcium Oxalate in Artificial Urine

Li¹,

Xu²,

Ouyang³

et al. 2014

Asian J. Chem.

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Urolithiasis is a chronic disease with an incidence increasing annually, may relapse after treatment 1. Calcium oxalate (CaC2O4) is the main component of urinary stones. Stone formation involves nucleation, growth, aggregation and adhesion process of urinary crystallites. The complexity of the internal environment in the human body, observing the formation processes of stones in situ is difficult. Therefore, we usually use the simulative method in vitro to study the nucleation, growth and aggregation of CaC2O4 and the influence of various inhibitors on these processes 1. These simulative systems contain water, urine, artificial urine, membrane mimetic system, surface of renal epithelial cells, etc 2-5. Nishio et al. 6 studied the inhibition effects of prothrombin fragment 1 and osteopontin on CaC2O4 crystallization in artificial urine, indicating that the inhibition effects of the two proteins on the growth rate of CaC2O4 were 27 and 32 %, respectively, when their concentration were 2 µg/mL. Wang et al. 7 studied the inhibition effect of phosphorylated osteopontin segment on nucleation and growth of calcium oxalate monohydrate (COM) crystals under near-physiological pH, temperature and ionic strength. When the concentration of phosphorylated OPN was 43.9 nm, the growth rate of CaC2O4•H2O

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Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

Karim,

Marghich,

Amrani

et al. 2024

Front. Chem.

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Ethnopharmacological relevanceIn Moroccan traditional medicine, plants from the Apiaceae family are widely utilized in folk medicine to treat various diseases associated with the digestive system. Ammodaucus leucotrichus plays an important role as an antispasmodic that has been traditionally used, especially to treat digestive tract diseases in children.Aim of the studyThe aim of this research was to verify the traditional use by assessing the relaxant and spasmolytic activities of A. leucotrichus essential oil (ALEO) and then comparing them to the effects and potency of the major constituent of ALEO, which is perillaldehyde.Materials and methodsThe in vitro evaluation of ALEO’s relaxant and spasmolytic effects was carried out on isolated rats and rabbit jejunum in an organ bath setup. Intestinal contractility was recorded using an isotonic transducer connected to an amplifier. GC/MS analysis was conducted to identify components within ALEO. Subsequently, these compounds underwent in silico absorption, toxicity, and molecular docking studies.ResultsGC/MS analysis of this essential oil studied revealed seven compounds, which account for 98.67% of the oil, with the dominance of two compounds, namely, perillaldehyde (91.12%) and limonene (6.33%). ALEO and its main compound, perillaldehyde, reversibly relaxed the basal tone of rabbit jejunum, with the IC50 values 158.68 ± 13.89 and 95.03 ± 0.93 μg/mL, respectively. Moreover, ALEO caused a dose-dependent spasmolytic effect on Carbachol (CCh) and KCl provoked jejunum contraction in rats. Furthermore, the decrease in contractions of pre-contracted jejunum by CCh was more pronounced for perillaldehyde compared to ALEO, with an IC50 value of 68.59 ± 6.57 μg/mL, which was half compared to that of ALEO. The pre-treatment of the tissue with concentrations ranging from 30 to 100 μg/mL caused a rightward and downward shift in the concentration–response curves for CaCl2 and CCh. These results suggest that the spasmolytic effect of ALEO is mediated possibly through a non-competitive antagonist of calcium channel or muscarinic receptors. Our results are confirmed by the fact that perillaldehyde exhibited the highest docking scores on muscarinic acetylcholine receptors (M2 and M3) and voltage-gated calcium channels, with D-limonene showing lower binding energies in comparison. These remarks confirm that the activity of ALEO is attributed to the presence of perillaldehyde. In addition, perillaldehyde exhibits a low degree of in silico acute toxicity and high percent of intestinal absorption.ConclusionIn summary, ALEO exhibits myorelaxant and antispasmodic effects by inhibiting muscarinic receptors and calcium channels, which can be attributed to the presence of perillaldehyde. This provides a scientific foundation for the traditional use of A. leucotrichus in treating gastrointestinal disorders and opens up possibilities for developing a more effective and less toxic drug-utilizing perillaldehyde.

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Effects of an aqueous extract fromAmmodaucus leucotrichuson calcium oxalate crystallizationin vitro

Cited by 4 publications

References 2 publications

Ethnomedicinal use, phytochemistry, pharmacology, and toxicology of Chamaerops humilis: A review

Ethnomedicinal use, phytochemistry, pharmacology, and toxicology of Chamaerops humilis: A review

Effect of Unitary, Binary and Ternary Carboxylates on Crystallization Kinetics of Calcium Oxalate in Artificial Urine

Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

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