Effects of ascorbic acid and ascorbic acid 2‐phosphate, a long‐acting vitamin C derivative, on the proliferation and differentiation of human osteoblast‐like cells

Takamizawa, Shinji; Maehata, Yojiro; Imai, Katsuyuki; Senoo, Haruki; Sato, Sadao; Hata, Ryu‐Ichiro

doi:10.1016/j.cellbi.2004.01.010

Cited by 136 publications

(119 citation statements)

References 27 publications

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“…The proliferation activity has often been reported to be inversely correlated to the cell differentiation, which includes the expression of collagen, ALP, and osteocalcin. 62 AA is a differentiation factor that attenuates cell proliferation 63 in the cell culture medium. The addition of AA probably masked the influence of the substrate's cell-growth properties on cell behavior; hence, on all tested surfaces, the differences in the expression of differentiation markers in cells equalized.…”

mentioning

confidence: 99%

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Lišková¹,

Babchenko²,

Varga³

et al. 2015

IJN

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Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings.

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confidence: 99%

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Lišková¹,

Babchenko²,

Varga³

et al. 2015

IJN

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“…IVC therapy is unlikely to have such severe side effects, and may act as an adjuvant to reduce side effects of other agents. Furthermore, ascorbic acid induces formation of collagen matrix and enhances osteoblast differentiation [50]. Since cancer patients are ascorbate deficient and have an abnormal demand for ascorbate due to oxidative stress, using AA as an adjuvant to support these processes may help prevent metastasis in prostate cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Changes in the rate of PSA progression and the level of alkaline phosphatase during high dose vitamin C treatment of patients with prostate cancer

Mikirova¹,

Hunninghake²

2017

FFHD

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Citation: Mikirova N., Hunninghake R., Changes in the rate of PSA progression and the level of alkaline phosphatase during high dose vitamin C treatment of patients with prostate cancer. Functional Foods in Health and Disease 2017; 7(7): 511-528 ABSTRACT Introduction: Intravenously administered vitamin C (IVC) may have anti-cancer and antiinflammatory properties. Many studies demonstrated evidence of a good safety profile of IVC treatments and improvement of the quality of life in cancer patients. IVC has been proposed as a treatment for cancer as an adjuvant in conjunction with other therapies. To investigate high dose ascorbic acid potential in treating prostate cancer, a retrospective study was conducted using clinical data from the Riordan Clinic database . Methods:We collected data, when available, on the following patient characteristics at diagnosis and during the courses of IVC therapy: age, tumor stage, Gleason score, serum prostate specific antigen (PSA) and alkaline phosphatase (ALP) levels, and location of metastases. In particular, PSA, ALP, and C-reactive protein (CRP) levels are analyzed in prostate cancer patients given IVC therapy during several years. Results:We found that PSA, CRP, and ALP correlate with tumor staging as measured by Gleason scores. Moreover, peak plasma ascorbate levels attained during the patients first IVC infusions are reduced in patients with elevated PSA and CRP levels. Tracking the changes in PSA and ALP with time in patients for whom data are available indicates that the rate of increase in these variables over time can be reduced by incorporating IVC therapy and by increasing the frequency of IVC treatments. Conclusion: There appeared to be a relation between the frequency of IVC treatments and the rate of PSA change, with PSA rate of growth decreasing as the frequency of IVC increases. Further research into the use of IVC in prostate cancer patients is warranted.

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“…1A), a stable phosphate derivative of L-ascorbic acid has been reported to stimulate growth of dermal papilla cells in vitro (Hata and Senoo, 1989;Takamizawa et al, 2004) and induce early conversion of telogen phase to anagen phase of hair cycle in experiment with mice (Sung et al, 2006). Furthermore, MAP treatment resulted in significant elongation of hair shafts in isolated hair follicles.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Methylsulfonylmethane on Hair Growth Promotion of Magnesium Ascorbyl Phosphate for the Treatment of Alopecia

Shanmugam¹,

Baskaran²,

Nagayya-Sriraman³

et al. 2009

Biomolecules and Therapeutics

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Fig. 1. The chemical structures of magnesium ascorbyl phosphate (MAP) and methylsulfonylmethane (MSM). (A) Magnesium ascorbyl phosphate (MAP). (B) Methylsulfonylmethane (MSM).Abstract -The purpose of this study was to evaluate the effect of methylsulfonylmethane (MSM) on hair growth promotion of magnesium ascorbyl phosphate (MAP) for the treatment of alopecia. Aqueous solutions of MAP 7.5% with or without MSM 1%, 5% or 10% were prepared and applied onto the depilated back skin of the male mice once a day for 20 days. The degree of hair growth was evaluated by visual scoring using hair growth quantification scale (0-5, 0 being initial state and 5 being complete hair growth). In vitro transdermal penetration and intradermal retention studies of MAP were performed with Franz diffusion cell using hairless mice skin. Hair growth in the group treated with the aqueous solution containing MAP 7.5% and MSM 10% was comparable to or better than the result in the group treated with minoxidil 5% solution. Hair growth promotion of MAP was dose-dependently increased by the presence of MSM used in combination with MAP 7.5% solution. The in vitro transdermal penetration of the MAP was decreased in proportion to the concentration of MSM. However, intradermal retention of MAP was profoundly and dose-proportionally increased as a function of MSM concentration, reaching 802 μg/cm 2 in the presence of MSM 10% (200-fold increase). The effect of MSM on hair growth promotion of MAP was dose-proportional to the concentration of MSM due to the enhanced intradermal retention of MAP in the presence of MSM. Therefore, topical application of MAP together with MSM appears to be useful for the treatment of alopecia.

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Effects of ascorbic acid and ascorbic acid 2‐phosphate, a long‐acting vitamin C derivative, on the proliferation and differentiation of human osteoblast‐like cells

Cited by 136 publications

References 27 publications

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Changes in the rate of PSA progression and the level of alkaline phosphatase during high dose vitamin C treatment of patients with prostate cancer

The Effect of Methylsulfonylmethane on Hair Growth Promotion of Magnesium Ascorbyl Phosphate for the Treatment of Alopecia

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