Effects of ascorbic acid, citric acid and silver nitrate on the growth of in vitro lily plantlets and reduction of browning

Abstract

Lily is an important cut-flower in Korea and world as well due to it's a variety of flower colors and various sizes of flowers. To develop elite lily cultivars, conventional breeding techniques have been used so far. However, an introduction of tissue culture system in mass propagation of bulbs and regeneration of shoots with a high efficiency is prerequisite at this moment. Especially, growth of bulbs and shoots as well as reduction of browning is critical factor to proliferate bulbs with shoots of superior lines or cultivar in lily. For this purpose, we tried to test whether ascorbic acid, citric acid and silver nitrate in medium to facilitate growth of bulbs and shoots as well as reduction of browning of bulb scales in lily. As a result, ascorbic acid and silver nitrate showed no significant differences compared to control plants in the growth of bulbs and shoots. When bulb scales were treated with 150 mg․l-1 of citric acid, formations of shoots and bulbs showed best result. While, bulb scale treated with 100 mg․l-1 of citric acid showed the growth of shoot and root as well as increasing of fresh weight compared to other treatments. Regarding the reduction of browning, 150 mg․l-1 of ascorbic acid showed the best result with the less than 2%. Although more experiments with several commercial varieties are needed in the future to establish mass propagation of bulbs of lily, results obtained in this study are supposed to provide the basic knowledge and contribution in tissue culture system of lily.

Optimal concentrations of plant growth regulators and AgNO3 for the improvement of regeneration efficiency in Chrysanthemum morifolium ‘Ohblang’

“…절편체의 갈변은 조직배양시 에틸렌 가스 및 페놀 화합물 에 의한 것으로, 기내 식물체 대량 증식에서 생육불량의 주 요 원인으로 작용하여 조직배양에서 큰 문제점으로 이어져 왔다. 해바라기 (Chraibi et al 1991), 딸기 (Qin et al 2005) 등 다 양한 식물에서 AgNO 3 를 이용한 갈변 방지 효과를 입증하였 고, 또한 나리 (Roh et al 2013)…”