Effects of asiaticoside on the expression of Smad protein by normal skin fibroblasts and hypertrophic scar fibroblasts

Qi, Shaohai; Xie, Ju Lin; Pan, Shu; Xu, Ying; Li, T.-Z.; Tang, Jinming; Liu, X.-S.; Shu, Bin; Liu, P.

doi:10.1111/j.1365-2230.2007.02636.x

Cited by 27 publications

(18 citation statements)

References 8 publications

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“…The combination effects are achieved via several mechanisms. Asiaticoside may inhibit the proliferation of VSMCs and fibroblasts, which is consistent with other studies (8,20,21). Asiaticoside upregulates Smad7 and TGF-β1, thus reducing synthesis of type I collagen.…”

Section: Discussionsupporting

confidence: 93%

“…Pan et al have demonstrated that asiaticoside inhibits scar fibroblast growth via the Smad signal pathway. The Smad7 protein and mRNA levels were reported to be increased in asiaticoside-treated fibroblasts, compared with control fibroblasts (8,21). It is likely that asiaticoside has different functions in different tissues, and has distinct tissue specificity.…”

Section: Discussionmentioning

confidence: 99%

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Combination treatment with asiaticoside and rapamycin: A new hope for in-stent restenosis

Guo

Fang

Zhang

et al. 2013

Experimental and Therapeutic Medicine

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The aim of this study was to investigate and characterize the efficacy and mechanism of action of asiaticoside in combination with rapamycin in the inhibition of in-stent restenosis (ISR). The effects of asiaticoside combined with rapamycin on cell proliferation in vitro were evaluated by MTT assay. The mRNA expression was analyzed by quantitative polymerase chain reaction (qPCR). Enzyme-linked immunosorbent assay (ELISA) was used to confirm protein synthesis. The cell growth inhibition rate in the combination group was significantly higher compared with those in the asiaticoside and rapamycin groups for human aortic fibroblasts (HAFs; 63.50±3.83, 53.06±8.10 and 60.34±4.9%, respectively) and human aortic smooth muscle cells (HASMCs; 33.12±1.35, 26.21±7.59 and 28.27±4.92, respectively; P<0.05). However, for human coronary artery endothelial cells (HCAECs), the cell growth inhibition rates in the combination, asiaticoside and rapamycin groups were 11.09±1.17, 26.22±4.24 and 34.80±2.80%, respectively (P<0.05), as detected by MTT assay. The qPCR assay showed that in the combination group the level of von Willebrand factor (vWF) mRNA was downregulated, while platelet endothelial cell adhesion molecule (PECAM-1) and endothelial nitric oxide synthase (eNOS) mRNAs were upregulated in HCAECs compared with the rapamycin group (P<0.05). Transforming growth factor (TGF)-β1 and TIMP1 mRNAs were downregulated while Smad7 and matrix metalloproteinase 1 (MMP1) mRNAs were upregulated in HAFs compared with the rapamycin and AT groups (P<0.05). The ELISA showed that the type I collagen level was significantly reduced in HASMCs and HAFs (P<0.05). The data suggest that asiaticoside combined with rapamycin may be effective in the reduction of ISR.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Combination treatment with asiaticoside and rapamycin: A new hope for in-stent restenosis

Guo

Fang

Zhang

et al. 2013

Experimental and Therapeutic Medicine

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“…Therefore, inhibition of proliferation with induction of apoptosis in HSFs is an important therapy for hypertrophic scars (Wu et al, 2011). There are several natural compounds reported on their proliferation-inhibiting and apoptosis-inducing effects in HSFs (Cao et al, 2009;Lin et al, 2013;Qi et al, 2008;Rawlins et al, 2006). Unfortunately, no mechanisms of action are investigated more deeply and systematically for these natural compounds.…”

Section: Discussionmentioning

confidence: 99%

Triggering of p38 MAPK and JNK Signaling is Important for Oleanolic Acid‐Induced Apoptosis via the Mitochondrial Death Pathway in Hypertrophic Scar Fibroblasts

Chen

Zhang

et al. 2014

Phytotherapy Research

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Hypertrophic scarring is characterized by collagen overproduction and excessive deposition of extracellular matrix. No consensus arises currently about the best therapeutics to produce complete and permanent improvement of scars with few side effects. In the present study, the mechanism of oleanolic acid (OA)-induced apoptosis in hypertrophic scar fibroblasts (HSFs) was investigated for the first time. OA activated the protein phosphorylation of p38 MAPK and JNK but not ERK. OA did not antagonize the inhibitory effects of SB203580 on p38 MAPK pathway activity but sharply enhanced JNK phosphorylation when HSFs were pretreated with SB203580. Similarly, the inhibition of JNK signal pathway activation by pretreatment with SP600125 facilitated the protein phosphorylation of p38 MAPK caused by OA. Inhibition of p38 MAPK and/or JNK by inhibitors significantly enhanced cell viability and OA only partially depressed the increased cell viability. Moreover, OA increased Bax translocation, MMP loss, mitochondrial cytochrome c and AIF release, Bax and caspase-3 protein expression and the ratio of Bax to Bcl-2, decreased Bcl-2 protein expression, and elevated the mRNA expression of Apaf-1, caspase-9, and capase-3. These results suggest that OA elicits apoptosis through triggering of p38 MAPK and JNK signaling and activation of the mitochondrial death pathway. OA might be a good and useful natural drug against hypertrophic scars.

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“…C. asiatica promotes balanced collagen production by increasing the production of type I collagen and decreasing myofibroblast production. It also helps to rearrange the collagen fiber [20][21][22]. Paper Mulberry extract contains and active ingredient -Kazinol F. It can inhibit the activity of tyrosinase synthesis and that lead to reduced melanin formation [23,24].…”

Section: Discussionmentioning

confidence: 99%

Prospective randomize-controlled comparison between silicone plus herbal extract gel versus Aloe vera gel for burn scar prophylaxis

2015

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Effects of asiaticoside on the expression of Smad protein by normal skin fibroblasts and hypertrophic scar fibroblasts

Abstract: Asiaticoside inhibits scarring probably by enhancing the expression of inhibitory Smad7, and is a potential treatment for scarring.

Cited by 27 publications

References 8 publications

Combination treatment with asiaticoside and rapamycin: A new hope for in-stent restenosis

Combination treatment with asiaticoside and rapamycin: A new hope for in-stent restenosis

Triggering of p38 MAPK and JNK Signaling is Important for Oleanolic Acid‐Induced Apoptosis via the Mitochondrial Death Pathway in Hypertrophic Scar Fibroblasts

Prospective randomize-controlled comparison between silicone plus herbal extract gel versus Aloe vera gel for burn scar prophylaxis

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