Effects of Aspirin or Basic Amino Acids on Collagen Cross-Links and Complications in NIDDM

Abstract

It is concluded that 1) low-dose aspirin may reduce glycooxidative damage in people with NIDDM, and 2) treatment may need to continue for more than 1 year before clinical status improves.

Antioxidative enzyme and glutathione S-transferase activities in diabetic rats exposed to long-term ASA treatment

“…It has been suggested that glycation of antioxidant enzymes due to hyperglycemia may be responsible for the reduction in GSHPx activity (Adachi et al, 1991;Wolff et al, 1991). It was suggested that low-dose ASA might reduce glycooxidative damage in diabetes (Contreras et al, 1997), mainly due to inhibition of post-Amadori Maillard reactions (a complex cascade of reactions initiated by glycation leading to production of "advanced glycation endproducts") (Hadley et al, 2001). In our experiment, we did not observe any hypoglycemic effect of the long-term ASA treatment (Table 1), but the ASA administration resulted in a slight decrease in the levels of glycated hemoglobin, presumably due to a competition between the two processes, protein glycosylation by a high glucose level and acetylation by ASA (Watala et al, 2006).…”

<i>L</i>-Lysine Reduces Nonenzymatic Glycation of Glomerular Basement Membrane Collagen and Albuminuria in Diabetic Rats

“…The mechanism by which lysine inhibits nonenzymatic glycation seems to be related to its ability to combine with glucose and make the latter unavailable to react with protein lysine residues. However, L -lysine in combination with L -arginine treatment for 1 year did not prevent either skin collagen cross-linking or glycation in type 2 diabetic patients [19]. This discrepancy between animal and human data may be related to the dosage of L -lysine and the duration of treatment.…”

“…These include acetylsalicylic acid [12, 13, 14, 15, 16, 17, 18, 19], L -lysine [14, 20], D -lysine [21, 22, 23], L -arginine [24], vitamin C [20, 25, 26, 27, 28, 29], vitamin E [30], bendazac [31], and aminoguanidine [4, 5, 6, 7, 8, 9, 10, 32, 33]. Other novel therapeutic agents have been shown to improve albuminuria and mesangial expansion in diabetic mice [34, 35]and rats [36], with type 2 diabetes melllitus.…”

A Random-Pattern Skin-Flap Model in Streptozotocin Diabetic Rats

“…23,33,34 Different types of microvascular and microcirculatory damage is present [35][36][37] (eg, induced hyperviscosity and hypercoagulability, 13,33,[38][39][40] increased oxidative damage, 21,41 damage of vital enzymes and proteins by nonenzymatic glycosylation). 32,41,42 Sorbitol accumulates in the intracellular area because of glucose's increased polyol pathway activity, which causes cellular swelling and damage with osmotic effect. 20,21,43 Similar studies showed that effect in scanning electron microscopic studies.…”