Effects of basic media and plant growth regulators on Direct-somatic embryogenesis induction in vegetative strains obtained from the tissue culture of digitalis purpurea

AL-Oqab, Mohammed Ahmed; Zaid, Salim; Al-Ammouri, Youssef

doi:10.9755/ejfa.2023.v35.i2.3008

Cited by 1 publication

(3 citation statements)

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“…The treatment containing 1.0 mg L -1 BA resulted in the greatest average wet and dried weight of the digitalis plant, as observed. The findings align with the results reported in references [13][14][15][16].…”

Section: Effect Of Ba On the Dry Weight Of Digitalis Plants In Vitro ...supporting

confidence: 93%

“…The increase in the average wet and dried weight of the digitalis plant was a direct consequence of the plant's enhanced height. The findings are consistent with those reported in reference [15] concerning the impact of gibberellin acid on the growth rate of the digitalis plant and the average number of branches and leaves that it produces.…”

Section: The Effect Of Ga3 On the Average Dry Weight (G) Of Digitalis...supporting

confidence: 93%

“…In 2013, [14] multiplied the shoots of digitalis plants ex vivo using various concentrations of growth regulators and discovered that the MS medium containing 0.75 mg L-1 BA produced the maximum average number of shoots, 13.4 shoots part -1 . The utilization of various concentrations of GA3 (0, 50, 100, 150, 200) micrograms was recommended by the researcher [15].L -1 . The quantity in micrograms is 200.L -1 produced the greatest mean number of branches, amounting to 16.8.…”

Section: Introductionmentioning

confidence: 99%

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Digitalis purpurea L. Propagated in Vitro with Various Hormones

Abdulraheem,

Noori,

Husni

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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The research was conducted in 2024 at the Department of Horticulture and Landscape Design, College of Agriculture, University of Kirkuk, IRAQ, plant cell and tissue culture laboratory. The seeds of digitalis plants, which were procured from the Spanish firm Semill Asfitto, were utilized in an investigation into the impact of gibberellic acid (GA3) and cytokine (BA) on increased vegetative growth characteristics and moist and dry weight, as well as to ascertain the most effective concentration of these substances. In accordance with a completely randomized design (CRD), ten replications of factorial experiments were utilized to conduct the experiments. The initial step was to sterilize the seeds, which were doused in 10% (v/v) sodium hypochlorate solution for five minutes prior to being planted in Mursch and Skoog (MS) medium devoid of growth regulators. Following the germination of the seeds and the cutting of the seedlings, the nodes were extracted for utilization in the multiplication experiments. A range of benzyl adenine (BA) concentrations (0.0, 1.0, 2.0, and 3.0 mg.L-1) and gibberellic acid (GA3) concentrations (0.0, 1.0, 2.0, and 3.0 mg.L-1) were utilized. Based on the findings, it can be concluded that the optimal concentration for multiplication is not 2.0 mg L-1 of BA, as the mean number of branches reached 2.40. The mean quantity of leaves produced per plant part-1 was 7.22. plant section 1. The concentration of gibberellic acid (GA3) that produced the best results for multiplication was 3.0 mg.

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Section: Effect Of Ba On the Dry Weight Of Digitalis Plants In Vitro ...supporting

confidence: 93%

Section: The Effect Of Ga3 On the Average Dry Weight (G) Of Digitalis...supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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