Effects of Bisphenol A on
 Foxl2
 gene expression and DNA damage in adult viviparous fish
 Goodea
 atripinnis

Cervantes-Camacho, Isabel; Guerrero-Estévez, Sandra Milena; López, María Fernanda; Alarcón-Hernández, Ernesto; López‐López, Eugenia

doi:10.1080/15287394.2020.1730282

Cited by 24 publications

(11 citation statements)

References 66 publications

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“…According to previous studies, these same observations were found Ctenopharyngodon [60], zebrafish [61], bighead carp [12], and Channa punctatus [62]. Moreover, clinical ailments in vertebrates [26,63,64] have also been reported. The findings of present study were observed which are also similar when Heteropneustes fossilis and C. carpio were exposed to different concentrations of insecticides [42,65].…”

Section: Discussionsupporting

confidence: 63%

“…Basically, these microscopic changes were due to gradual increase in lipid peroxidation and increased stress biomarkers (ROSs). However, it is well documented in previous reports that exposure of animals to different toxicants causes detoxification in their bod-ies by increasing the level of ROS which ultimately results in less production of antioxidant enzymes [12,26,86]. Previously toxic effects of BPA on brain of fish have also been reported [87].…”

Section: Discussionmentioning

confidence: 98%

“…Among aquatic animals, fish are highly sensitive to different pollutants including BPA which is mainly absorbed through the skin, gills, and alimentary route and get absorbed into the body tissues by disrupting the physiological and biochemical processes. A number of studies regarding effects of BPA on the growth, behavior, morphological characters, genotoxicity, biochemical, and histological changes in fish were reported [23][24][25][26][27]. BPA may also change the gene expression pattern throughout the development of body organs and is strongly influenced by both genetic and environmental factors [28,29].…”

Section: Introductionmentioning

confidence: 99%

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Bisphenol A Induces Histopathological, Hematobiochemical Alterations, Oxidative Stress, and Genotoxicity in Common Carp (Cyprinus carpio L.)

Afzal

Ahmad

Hussain

et al. 2022

Oxidative Medicine and Cellular Longevity

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Bisphenol A (BPA) is one of the environmental endocrine disrupting toxicants and is widely used in the industry involving plastics, polycarbonate, and epoxy resins. This study was designed to investigate the toxicological effects of BPA on hematology, serum biochemistry, and histopathology of different organs of common carp (Cyprinus carpio). A total of 60 fish were procured and haphazardly divided into four groups. Each experimental group contained 15 fish. The fish retained in group A was kept as the untreated control group. Three levels of BPA 3.0, 4.5, and 6 mg/L were given to groups B, C, and D for 30 days. Result indicated significant reduction in hemoglobin (Hb), lymphocytes, packed cell volume (PCV), red blood cells (RBC), and monocytes in a dose-dependent manner as compared to the control group. However, significantly higher values of leucocytes and neutrophils were observed in the treated groups ( P < 0.05 ). Results on serum biochemistry revealed that the quantity of glucose, cholesterol, triglycerides, urea, and creatinine levels was significantly high ( P < 0.05 ). Our study results showed significantly ( P < 0.05 ) increase level of oxidative stress parameters like reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) and lower values of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) in treated groups (4.5 mg/L and 6 mg/L)) in the brain, liver, gills, and kidneys. Our study depicted significant changes in erythrocytes (pear shaped erythrocytes, leptocytes, microcytes, spherocytes, erythrocytes with broken, lobed, micronucleus, blabbed, vacuolated nucleus, and nuclear remnants) among treated groups (4.5 mg/L and 6 mg/L). Comet assay showed increased genotoxicity in different tissues including the brain, liver, gills, and kidneys in the treated fish group. Based on the results of our experiment, it can be concluded that the BPA exposure to aquatic environment is responsible for deterioration of fish health, performance leading to dysfunction of multiple vital organs.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Bisphenol A Induces Histopathological, Hematobiochemical Alterations, Oxidative Stress, and Genotoxicity in Common Carp (Cyprinus carpio L.)

Afzal

Ahmad

Hussain

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Thus, the FOXL2 transcription factor is crucial for ovarian development and function (Georges et al, 2014) and its role in ovarian differentiation seems to be highly conserved among vertebrates (Cocquet et al, 2002). The effect of EACs on ovarian FOXL2 expression has been extensively studied in fish, and upregulation of FOXL2 has been demonstrated after exposure of ovaries to compounds with estrogenic activity (Wang et al, 2012, Cervantes-Camacho et al, 2020. Of note, it was shown that FOXL2 regulates, directly and indirectly, genes involved in cell proliferation and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Effects of Sex Steroid Receptor Agonists and Antagonists on the Expression of the FOXL2 Transcription Factor and its Target Genes AMH and CYP19A1 in the Neonatal Porcine Ovary

Witek

Marek

Grzesiak

et al. 2022

Annals of Animal Science

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Recently, we have demonstrated that neonatal exposure to androgen and estrogen agonists or antagonists influenced the number of ovarian follicles in piglets. Since the FOXL2 transcription factor is required for proper ovarian follicle formation and activation, the objective of the study was to examine effects of exposure of the neonatal porcine ovary to testosterone propionate (TP; an androgen), flutamide (FLU; an antiandrogen), 4-tert-octylphenol (OP; compound with estrogenic activity), ICI 182,780 (ICI; an antiestrogen), and methoxychlor (MXC; compound with estrogenic, antiestrogenic and antiandrogenic properties) on FOXL2 expression and expression of its target genes, AMH and CYP19A1. Piglets were injected subcutaneously with TP, FLU, OP, ICI, MXC, or corn oil (control) between postnatal days 1 and 10 (n = 4/each group). Ovaries were excised from the 11-day-old piglets and the expression of FOXL2, AMH and CYP19A1 were examined using immunohistochemistry and/or real-time PCR and Western blot. FOXL2 was localized in stroma cells surrounding egg nests and in granulosa cells. TP, OP and MXC increased both FOXL2 and AMH mRNAs, while FLU and ICI decreased CYP19A1 mRNA. The increased FOXL2 protein abundance was found in all examined groups. In addition, TP, OP, ICI and MXC increased AMH protein abundance, while TP, FLU and OP decreased CYP19A1 protein abundance. In conclusion, neonatal exposure to sex steroid receptor agonists and antagonists increased FOXL2 expression at mRNA and/or protein levels and affected FOXL2 target genes in the ovaries of 11-day-old piglets. Therefore, it seems that impaired ovarian folliculogenesis induced by altered steroid milieu during the neonatal development period in pigs may, at least in part, involve FOXL2.

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“…In a 60-day dietary exposure study, EE2fed female largemouth bass (Micropterus salmoides) exhibited decreased plasma VTG, hepatosomatic index (HSI) and GSI, and increased hepatic and gonadal er, and gonadal aromatase (cyp19a) transcripts (138). Induction of forkhead box protein L2 (foxl2), a gene which play a role in ovarian differentiation and in maintenance of ovarian functions in mammals and fish (200)(201)(202), was observed after 14 and 28 days of exposure to BPA in females, and after 28 days in males of killifish (Goodea atripinnis) (162). Breeding groups of zebrafish treated with BPA for 15 days exhibited increased egg production, reduced fertilization success, alterations in levels of genes involved in reproductive function and epigenetic processes and reduction in global DNA methylation in both liver and gonad tissue (150).…”

Section: Reproductionmentioning

confidence: 99%

Experimental Approaches for Characterizing the Endocrine-Disrupting Effects of Environmental Chemicals in Fish

2021

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Increasing industrial and agricultural activities have led to a disturbing increase of pollutant discharges into the environment. Most of these pollutants can induce short-term, sustained or delayed impacts on developmental, physiological, and behavioral processes that are often regulated by the endocrine system in vertebrates, including fish, thus they are termed endocrine-disrupting chemicals (EDCs). Physiological impacts resulting from the exposure of these vertebrates to EDCs include abnormalities in growth and reproductive development, as many of the prevalent chemicals are capable of binding the receptors to sex steroid hormones. The approaches employed to investigate the action and impact of EDCs is largely dependent on the specific life history and habitat of each species, and the type of chemical that organisms are exposed to. Aquatic vertebrates, such as fish, are among the first organisms to be affected by waterborne EDCs, an attribute that has justified their wide-spread use as sentinel species. Many fish species are exposed to these chemicals in the wild, for either short or prolonged periods as larvae, adults, or both, thus, studies are typically designed to focus on either acute or chronic exposure at distinct developmental stages. The aim of this review is to provide an overview of the approaches and experimental methods commonly used to characterize the effects of some of the environmentally prevalent and emerging EDCs, including 17 α-ethinylestradiol, nonylphenol, BPA, phthalates, and arsenic; and the pervasive and potential carriers of EDCs, microplastics, on reproduction and growth. In vivo and in vitro studies are designed and employed to elucidate the direct effects of EDCs at the organismal and cellular levels, respectively. In silico approaches, on the other hand, comprise computational methods that have been more recently applied with the potential to replace extensive in vitro screening of EDCs. These approaches are discussed in light of model species, age and duration of EDC exposure.

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Effects of Bisphenol A on Foxl2 gene expression and DNA damage in adult viviparous fish Goodea atripinnis

Cited by 24 publications

References 66 publications

Bisphenol A Induces Histopathological, Hematobiochemical Alterations, Oxidative Stress, and Genotoxicity in Common Carp (Cyprinus carpio L.)

Bisphenol A Induces Histopathological, Hematobiochemical Alterations, Oxidative Stress, and Genotoxicity in Common Carp (Cyprinus carpio L.)

Effects of Sex Steroid Receptor Agonists and Antagonists on the Expression of the FOXL2 Transcription Factor and its Target Genes AMH and CYP19A1 in the Neonatal Porcine Ovary

Experimental Approaches for Characterizing the Endocrine-Disrupting Effects of Environmental Chemicals in Fish

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