Effects of blue LED light irradiation on pigment metabolism of ethephon-degreened mandarin fruit

Zhang, Yuan; Deng, Lili; Yin, Baofeng; Yao, Shixiang; Zeng, Kaifang

doi:10.1016/j.postharvbio.2017.08.005

Cited by 38 publications

(28 citation statements)

References 54 publications

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“…The proteins were screened with a fold-change value > 1. 5 Figure S3). The heatmap/hierarchical clustering analysis was conducted for all the identified proteins and DEPs using the pheatmap package in R language.…”

Section: Deps Identified and Functional Analysismentioning

confidence: 99%

“…Color is one of the quality factors of cash crops and agricultural products, and color change associated with carotenoid and chlorophyll metabolism is one of the most obvious phenomena in postharvest vegetative organs during curing and storage. A substantial amount of research has been carried out to comprehend this fundamental postharvest physiological process to improve the commercial value of agricultural goods during the past few decades [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…Parameters of the CIEL*a*b color coordinate include lightness L* (positive white and negative black), and two chromatic components a* (positive red and negative green) and b* (positive yellow and negative blue) [2,11]. Color change is one of the most dramatic events occurring in plant postharvest organs during curing and storage [2,5,23]. Pigments are compounds that absorb subsets of the visible spectrum, transmitting and reflecting back only what they do not absorb, and causing the tissue to be perceived as the reflected colors [24].…”

Section: Introductionmentioning

confidence: 99%

“…iTRAQ (isobaric tags for relative and absolute quantification), a high-throughput proteomic technology, is one platform for comparing changes in the abundance of specific proteins among different samples [8,29,38]. Although new advances have been made in our understanding of pigment metabolism and color change in plant organs [4][5][6]13,23], fewer studies have focused specifically on their molecular mechanisms in postharvest tobacco leaves during curing. In this study, iTRAQ-based proteomic analysis was employed to identify important regulators in pigment metabolism pathways and elucidate the molecular mechanism of pigment metabolism and color change in tobacco leaves during the yellowing stage (0 h, 48 h, 72 h).…”

Section: Introductionmentioning

confidence: 99%

“…(B) Number of peptides that matched proteins.2.6. DEPs Identified and Functional AnalysisThe proteins were screened with a fold-change value > 1 5. or < 0.67 and a Q-value of < 0.05.…”

mentioning

confidence: 99%

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Comparative Proteomic Analysis by iTRAQ Reveals that Plastid Pigment Metabolism Contributes to Leaf Color Changes in Tobacco (Nicotiana tabacum) during Curing

Guo²,

Adil

et al. 2020

IJMS

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Tobacco (Nicotiana tabacum), is a world’s major non-food agricultural crop widely cultivated for its economic value. Among several color change associated biological processes, plastid pigment metabolism is of trivial importance in postharvest plant organs during curing and storage. However, the molecular mechanisms involved in carotenoid and chlorophyll metabolism, as well as color change in tobacco leaves during curing, need further elaboration. Here, proteomic analysis at different curing stages (0 h, 48 h, 72 h) was performed in tobacco cv. Bi’na1 with an aim to investigate the molecular mechanisms of pigment metabolism in tobacco leaves as revealed by the iTRAQ proteomic approach. Our results displayed significant differences in leaf color parameters and ultrastructural fingerprints that indicate an acceleration of chloroplast disintegration and promotion of pigment degradation in tobacco leaves due to curing. In total, 5931 proteins were identified, of which 923 (450 up-regulated, 452 down-regulated, and 21 common) differentially expressed proteins (DEPs) were obtained from tobacco leaves. To elucidate the molecular mechanisms of pigment metabolism and color change, 19 DEPs involved in carotenoid metabolism and 12 DEPs related to chlorophyll metabolism were screened. The results exhibited the complex regulation of DEPs in carotenoid metabolism, a negative regulation in chlorophyll biosynthesis, and a positive regulation in chlorophyll breakdown, which delayed the degradation of xanthophylls and accelerated the breakdown of chlorophylls, promoting the formation of yellow color during curing. Particularly, the up-regulation of the chlorophyllase-1-like isoform X2 was the key protein regulatory mechanism responsible for chlorophyll metabolism and color change. The expression pattern of 8 genes was consistent with the iTRAQ data. These results not only provide new insights into pigment metabolism and color change underlying the postharvest physiological regulatory networks in plants, but also a broader perspective, which prompts us to pay attention to further screen key proteins in tobacco leaves during curing.

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Section: Deps Identified and Functional Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…(B) Number of peptides that matched proteins.2.6. DEPs Identified and Functional AnalysisThe proteins were screened with a fold-change value > 1 5. or < 0.67 and a Q-value of < 0.05.…”

mentioning

confidence: 99%

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Comparative Proteomic Analysis by iTRAQ Reveals that Plastid Pigment Metabolism Contributes to Leaf Color Changes in Tobacco (Nicotiana tabacum) during Curing

Guo²,

Adil

et al. 2020

IJMS

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Low and high storage temperature inhibited the coloration of mandarin fruit (Citrus unshiu Marc.) with different mechanism

Cao

et al. 2022

J Sci Food Agric

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BACKGROUND Peel color regulated by pigment metabolism is one of the most crucial indicators affecting the commodity values of citrus fruit. Storage temperature is a vital environmental factor that regulates the fruit pigmentation. RESULTS Results showed that the peel coloring process was significantly inhibited when mandarin fruit were stored at 5 and 32 °C with normal coloring at 25 °C as the control. However, the inhibitive mechanisms of 5 and 32 °C storage were different. At 5 °C, higher levels of CcNYC and CcCHL2 were detected, which indicated that 5 °C induces the circulation of chlorophyll rather than inhibits chlorophyll degradation. CcPSY2, CcCHYB, and CcZEP exhibited higher expression levels in fruit stored at 5 °C, which accelerated the accumulation of carotenoids. In fruit stored at 32 °C, CcNYC, CcPAO, and CcCHL2 exhibited lower expression levels than those fruit stored at 5 °C, and the expressions of CcPSY2, CcCHYB, and CcZEP were down regulated, implying the carotenoid synthesis was suppressed. CONCLUSION Storage at 5 °C inhibited the postharvest coloring of mandarin fruit mainly by activating the cycle of chlorophyll, although it promotes the accumulation of carotenoids at the same time, but chlorophyll covers the color of carotenoids. Storage at 32 °C inhibited mandarin fruit coloring mainly by inhibiting the degradation of chlorophyll. Compared with the change of individual chlorophyll or carotenoid content, the change of the ratio of chlorophyll and carotenoid had a more important role in the coloration of mandarin fruit. This research offers valuable details for understanding the effect of temperature on the coloring process of postharvest citrus fruit. © 2022 Society of Chemical Industry.

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The response of Midknight Valencia oranges to ethephon degreening varies in the turning and regreening stages

Li,

Ai,

Zeng

et al. 2024

J Sci Food Agric

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BACKGROUNDLate‐ripening citrus plays an important role in the stability of the global citrus industry. However, the regreening phenomenon in Valencia oranges impacts the peel color and commercial value. Ethylene degreening is an effective technique to improve the color of citrus fruits, but this effect may be delayed in regreened oranges. To better clarify this phenomenon, plastid morphology, pigment and phytohormone content in ethephon‐degreened Midknight Valencia oranges harvested in different stages were evaluated.RESULTSResults showed that in fruits harvested at the turning stage, ethephon degreening treatment induced a chloroplast‐to‐chromoplast transition, and chlorophyll degradation and carotenoid accumulation were accelerated. Conversely, in fruits harvested at the regreening stage, the changes in plastid morphology were minimal, with delayed changes in chlorophyll and carotenoids. Genes related to ethylene biosynthesis and signaling pathways supported these responses. Variations in endogenous auxin, jasmonic acid, abscisic acid and gibberellins could partially explain this phenomenon.CONCLUSIONThe response of Midknight Valencia oranges to ethephon degreening was delayed in the regreening stage, possibly due to the dynamic variations in endogenous phytohormones. © 2024 Society of Chemical Industry.

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Effects of blue LED light irradiation on pigment metabolism of ethephon-degreened mandarin fruit

Cited by 38 publications

References 54 publications

Comparative Proteomic Analysis by iTRAQ Reveals that Plastid Pigment Metabolism Contributes to Leaf Color Changes in Tobacco (Nicotiana tabacum) during Curing

Comparative Proteomic Analysis by iTRAQ Reveals that Plastid Pigment Metabolism Contributes to Leaf Color Changes in Tobacco (Nicotiana tabacum) during Curing

Low and high storage temperature inhibited the coloration of mandarin fruit (Citrus unshiu Marc.) with different mechanism

The response of Midknight Valencia oranges to ethephon degreening varies in the turning and regreening stages

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