2020
DOI: 10.3390/su12239833
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Effects of Chemical and Solar Soil-Disinfection Methods on Soil Bacterial Communities

Abstract: The actctivated carbon + solar radiation membrane is an eco-friendly soil disinfestations method for managing soil-borne plant pathogens. However, little was known the impact of Activated carbon + solar radiation membrane on bacterial community structure in strawberry production systems under field conditions. A comprehensive evaluation of the impacts of different soil disinfection methods on the bacterial community structure is fundamental to understand the role of disinfection in maintaining soil health. The… Show more

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Cited by 5 publications
(2 citation statements)
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“…DNA extraction, PCR amplification, Illumina MiSeq sequencing, and data processing were performed as described by Yun et al [ 21 ], with minor modifications. Each PCR reaction system included 12.5 μL of 2× Taq Plus Master Mix, 3 μL of BSA (2 ng/μL), 1 μL of forward primer (5 μM), 1 μL of reverse primer (5 μM), 2 μL of DNA template, and 5.5 μL of double-distilled water.…”
Section: Methodsmentioning
confidence: 99%
“…DNA extraction, PCR amplification, Illumina MiSeq sequencing, and data processing were performed as described by Yun et al [ 21 ], with minor modifications. Each PCR reaction system included 12.5 μL of 2× Taq Plus Master Mix, 3 μL of BSA (2 ng/μL), 1 μL of forward primer (5 μM), 1 μL of reverse primer (5 μM), 2 μL of DNA template, and 5.5 μL of double-distilled water.…”
Section: Methodsmentioning
confidence: 99%
“…Soil-borne pathogens were separated according to the method of Yun et al [27]: 5 g of a soil sample was added to 95 mL of 0.7‰ sterilized agar water, and then the soil suspension was obtained by shaking in a shaker for 30 min. On the aseptic operating table, 2.5 mL of medium B component was mixed with 47.5 mL of medium A component, then 1 mL of the soil suspension was added, shaken well, and poured evenly into the petri dish.…”
Section: Soil-borne Pathogensmentioning
confidence: 99%