Effects of Compound Active Peptides on Protecting Liver and Intestinal Epithelial Cells from Damages and Preventing Hyperglycemia

Abstract

Active peptides have good effectiveness in controlling or preventing many diseases. Compound active peptides (CAP) obtained from animal, plant, and sea food proteins were used in this study to explore their effects on antioxidation, anti-inflammation, and antihyperglycemia in vitro and in vivo. The results demonstrated that 10 μg/mL CAP could increase cell viability (P<0.05) and decrease reactive oxygen species (ROS) levels and cell apoptosis (P<0.05) when WRL68 cells were induced by H2O2 for 6 h. Moreover, incubation with 20 μg/mL CAP for 6 h significantly increased cell viability and Bcl-2 expression level (P<0.05) and decreased expression levels of IL-6, IL-8, TNF-α, Bax, and Caspase 3 and the ratio of Bax/Bcl-2 (P<0.05) when swine jejunal epithelial cells (IPEC-J2) were induced by deoxynivalenol (DON). In addition, adding CAP individually or combined with Liuweidihuang pills (LDP, Chinese medicine) and low-dose glibenclamide could lower blood glucose levels in alloxan-induced hyperglycemic model mice. These results suggested that CAP was probably a beneficial ingredient for alleviating H2O2-induced oxidative stress and DON-induced cell inflammation and apoptosis and preventing hyperglycemia.

Stevioside Improves Antioxidant Capacity and Intestinal Barrier Function while Attenuating Inflammation and Apoptosis by Regulating the NF-κB/MAPK Pathways in Diquat-Induced Oxidative Stress of IPEC-J2 Cells

“…Oxidative stress can trigger an inflammatory response, which in turn can directly induce oxidative stress [52]. The production of pro-inflammatory cytokines, such as IL-6, IL-8, and TNF-α, which are often representative hallmarks of an inflammatory response [53].…”

Oxidative stress in human gingival fibroblasts from periodontitis versus healthy counterparts

“…Reactive oxygen species (ROS) are major normal byproducts of aerobic metabolism, and their levels are highly increased under oxidative stress conditions (Xu et al, 2020). The increased levels of ROS usually suggest activated mitochondrial oxidative phosphorylation, led to the accumulation of inflammatory responses (Li & Qin, 2018).…”

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Reactive oxygen species (ROS) are major normal byproducts of aerobic metabolism, and their levels are highly increased under oxidative stress conditions (Xu et al, 2020). The increased levels of ROS usually suggest activated mitochondrial oxidative phosphorylation, led to the accumulation of inflammatory responses (Li & Qin, 2018).Many factors such as periodontal pathogenic bacteria and the host immune response contributing to chronic periodontitis (CP) are associated with oxidative stress (Chen et al, 2019).

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