Effects of compression force on fibroblast growth factor‐2 and receptor activator of nuclear factor kappa B ligand production by periodontal ligament cells <i>in vitro</i>

Nakajima, Ryo; Yamaguchi, Masaru; Kojima, Tadashi; Takano, M.; Kasai, Kazuhiko

doi:10.1111/j.1600-0765.2007.01008.x

Cited by 51 publications

(42 citation statements)

References 54 publications

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“…The results of the current study indicate that various fibroblastic cells naturally produce OPG, thereby supporting the inhibitory roles of fibroblasts on osteoclastogenesis. There are also significant findings showing that mechanical force induces the production of both OPG and RANKL by PLF, while OPG is predominant over RANKL [Yamamoto et al, 2006;Nakajima et al, 2008;Kook et al, 2009]. Importantly, however, the results from GCF analysis showed that the levels of RANKL are naturally higher than those of OPG in this cell type.…”

Section: Discussionmentioning

confidence: 68%

Human periodontal ligament fibroblasts stimulate osteoclastogenesis in response to compression force through TNF-α-mediated activation of CD4+ T cells

2011

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Periodontal ligament fibroblasts (PLF) sense and respond to mechanical stimuli and participate in alveolar bone resorption during orthodontic treatments. This study examined how PLF influence osteoclastogenesis from bone marrow-derived macrophages (BMM) after application of tension or compression force. We also investigated whether lymphocytes could be a primary stimulator of osteoclastic activation during alveolar bone remodeling. We found that mechanical forces inhibited osteoclastic differentiation from BMM in co-cultures with PLF, with PLF producing predominantly osteoprotegerin (OPG) rather than receptor activator of nuclear factor-kappaB (NF-κB) ligand (RANKL). In particular, PLF increased the expression of tumor necrosis factor (TNF)-α in response to compression. Additional experiments showed the presence of CD4- and B220-positive cells with a subsequent increase in tartrate-resistant acid phosphatase (TRAP)-positive cells and RANKL expression only at the compression side of the force-subjected periodontal tissues. Exogenous TNF-α increased the number of TRAP-positive cells and pit formation in the co-cultures of BMM with Jurkat, but not with BJAB cells and this effect was almost completely inhibited by antibodies to TNF-α or TNF receptor. Collectively, the current findings suggest that PLF secrete relatively higher levels of TNF-α at the compression side than at the tension side and this imbalance leads to RANKL expression by activating CD4+ T cells, thereby facilitating bone resorption during orthodontic tooth movement.

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Section: Discussionmentioning

confidence: 68%

Human periodontal ligament fibroblasts stimulate osteoclastogenesis in response to compression force through TNF-α-mediated activation of CD4+ T cells

2011

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“…They also performed an in vitro study and reported that compression stressed PDL cells increased the secretion of RANKL in a time-dependent manner but decreased the secretion of OPG in a time-dependent manner. Nakajima et al 33 carried out a similar compressive stimulation study on PDL cells. They also reported that RANKL production was elevated in a time and force-dependent manner up to 4 g/cm 2 , the maximal force that they used.…”

Section: Discussionmentioning

confidence: 99%

Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cells

et al. 2009

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Objective: The aim of this study was to determine if human PDL cells can produce osteoclastogenic mRNA and examine how compressive stress affects the expression of osteoclastogenic mRNA in human PDL cells. Methods: Human PDL cells were obtained from biscupids extracted for orthodontic treatment. The compressive force was adjusted by increasing the number of cover glasses. PDL cells were subjected to a compressive force of 0.

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“…The compressive force is adjusted by adding lead granules to the bottle to allow PDLCs to be subjected to 2.0 g/cm 2 of compressive force, and the force is maintained for 0.5, 3, 6, or 12 hours, respectively. 3,18 These time points were selected according to the method described in our previously published article. 19 Two and a half microliters of GsMTx4 (Abcam, San Francisco, CA, USA), a specific inhibitor of cationic mechanosensitive ion channels, was added, and 0.8 ng/mL of TNF-a (R&D, Shanghai, China) was added to the indicated groups.…”

Section: Mechanical Stimulation: Application Of Static Compressive Forcementioning

confidence: 99%

“…This method comprises a modification of the method described by Hiroyuki and Li. 3,5,18 Briefly, a cover glass and a glass bottle are placed on the monolayer hPDLCs. The compressive force is adjusted by adding lead granules to the bottle to allow PDLCs to be subjected to 2.0 g/cm 2 of compressive force, and the force is maintained for 0.5, 3, 6, or 12 hours, respectively.…”

Section: Mechanical Stimulation: Application Of Static Compressive Forcementioning

confidence: 99%

Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells

Jin

Wang

et al. 2015

The Angle Orthodontist

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Objective: To evaluate the function of Piezo1, an evolutionarily conserved mechanically activated channel, in periodontal ligament (PDL) tissue homeostasis under compressive loading. Materials and Methods: Primary human PDL cells (hPDLCs) were isolated, cultured, and then subjected to 2.0 g/cm 2 static compressive loading for 0.5, 3, 6, and 12 hours, respectively. The expressions of Piezo1 and osteoclastogenesis marker gene were assessed by semiquantitative reverse transcription-polymerase chain reaction. In addition, Piezo1 inhibitor, GsMTx4, was used to block the function of Piezo1, and tumor necrosis factor-a was also used as a positive control. After 12 hours of compressive loading the PDLCs were co-cultured with murine monocytic cell line RAW264.7. Immunofluorescence, western blot, enzyme-linked immunosorbent assay, and tartrate-resistant acid phosphatase staining were also used to test the potency of PDLCs to induce osteoclastogenesis and the activation of nuclear factor (NF)-kB. Results: Piezo1, cyclooxygenase-2, receptor activator of NF-kB ligand, and prostaglandin E2 were significantly upregulated under static compressive stimuli. GsMTx4 repressed osteoclastogenesis in the mechanical stress-pretreated PDLCs-RAW264.7 co-culture system. Furthermore, NF-kB signaling pathway was involved in the mechanical stress-induced osteoclastogenesis. Conclusions: Piezo1 exerts a transduction role in mechanical stress-induced osteoclastogenesis in hPDLCs. (Angle Orthod. 2015;85:87-94.)

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Effects of compression force on fibroblast growth factor‐2 and receptor activator of nuclear factor kappa B ligand production by periodontal ligament cells in vitro

Abstract: Fibroblast growth factor-2 may be partly involved in osteoclastogenesis during orthodontic tooth movement.

Cited by 51 publications

References 54 publications

Human periodontal ligament fibroblasts stimulate osteoclastogenesis in response to compression force through TNF-α-mediated activation of CD4+ T cells

Human periodontal ligament fibroblasts stimulate osteoclastogenesis in response to compression force through TNF-α-mediated activation of CD4+ T cells

Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cells

Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells

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