Effects of CPP-ACP with sodium fluoride on inhibition of bovine enamel demineralization: A quantitative assessment using micro-computed tomography

Hamba, Hidenori; Nikaido, Toru; Inoue, Go; Sadr, Alireza; Tagami, Junji

doi:10.1016/j.jdent.2011.03.005

Cited by 102 publications

(107 citation statements)

References 47 publications

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“…Briefly, the powder and liquid were thoroughly mixed using a micro-brush, applied to the dentin surface, and cured by irradiation with a light activation unit (Optilux 501, Kerr Corporation, Orange, CA, USA) at the intensity of 500 mW/cm 2 for 10 s. The specimens in the control group were left uncovered. All specimens were immersed individually in 10 mL acidic demineralizing buffer solution (2.2 mmol/L CaCl 2 , 2.2 mmol/L NaH2PO4, and 50 mmol/L acetic acid adjusted to pH 4.5 with NaOH) [22][23][24] for 3 d at 37ºC. The demineralization buffer was changed every 24 h. Three specimens were tested for each group.…”

Section: Specimen Preparation For Micro-ct Scanningmentioning

confidence: 99%

“…The micro-CT system can generate polychromatic X-rays with a cone-beam geometry. A 0.2-mm-thick brass (Cu-Zn) filter was installed in the beam path to reduce beam-hardening effect 24,25) . The specimen was mounted on a computer-controlled turntable with the treated dentin surface parallel to the X-ray beam, and was covered with a wet cotton roll to prevent desiccation of the surface.…”

Section: Micro-ct Scanningmentioning

confidence: 99%

“…Demineralization of the tooth surface can be investigated through various methods including contact microradiography 27,28) , polarized light microscopy 29) , nanoindentation testing 24) and confocal laser microscopy 24,30) , among which contact microradiography is accepted as the "golden standard" technique for the determination of mineral content and caries lesion depth. However, specimen preparation in all these methods is destructive and time consuming.…”

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confidence: 99%

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Effects of a coating resin containing S-PRG filler to prevent demineralization of root surfaces

Imazato

Chen

et al. 2012

Dent. Mater. J.

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The purpose of this study was to evaluate the ability of a coating material containing the surface pre-reacted glass-ionomer (S-PRG) filler to protect the root from demineralization in vitro. The proprietary coating resin containing the S-PRG filler (PRG Barrier Coat) was applied to human root dentin and immersed in acid buffer at pH 4.5 for 3 d. Demineralization was evaluated by micro-CT scanning and the dentin-material interface observed by scanning electron microscopy. The ability of the coating resin to modify acid production by Streptococcus mutans was investigated by monitoring pH using an ion-sensitive field-effect transistor pH electrode. Application of PRG Barrier Coat produced a coating layer with the thickness of approximately 200 µm and completely inhibited demineralization. The bacteria-induced pH fall at the material surface was significantly inhibited. We conclude that S-PRG fillercontaining coating resin may be an effective material for protecting exposed root from both chemical and biological challenges. Keywords: Resin coating, Root caries, Demineralization, S-PRGThe second and third authors contributed equally to this work.

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Section: Specimen Preparation For Micro-ct Scanningmentioning

confidence: 99%

Section: Micro-ct Scanningmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of a coating resin containing S-PRG filler to prevent demineralization of root surfaces

Imazato

Chen

et al. 2012

Dent. Mater. J.

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“…Some authors have concluded that the fluorine is able to increase the remineralization potential of CPP-ACP. 7,8 Few studies that have assessed treatment of sound dentin with CPP-ACP followed by adhesive procedures, noted that the CPP-ACP pastes do not decrease the bond strength. 9,10,11 Alternatives aiming at improving the quality of the adhesive interface have been considered by some authors.…”

Section: Introductionmentioning

confidence: 99%

The effect of CPP-ACP and Nd:YAG laser on the bond strength of softened dentin

et al. 2015

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The purpose of this study was to investigate the effect of CPP-ACP treatment and Nd:YAG laser on microtensile bond strength (µTBS) of softened dentin. Sixty samples were obtained from thirty sound third molars. All samples were submitted to dentin softening procedure, by the immersion of the specimens in 30 mL of Sprite Zero for 30min. Afterwards, the samples were randomly divided according to the CPP-ACP treatment: CG-Control group; MP-treated with CPP-ACP paste (MI Paste); MPP-treated with CPP-ACP+900 ppm NaF paste (MI Paste Plus). Each group was further divided according to bonding procedure: NL-No laser; L-Laser irradiation after adhesive application and before polymerization. The laser parameters used were 1.4 W, 10 Hz, 140 mJ/pulse, with an optic fiber of 320 µm, generating energy of 174 J/cm 2 per pulse. All samples were restored with Clearfil SE Bond/Filtek Z350 XT. After 24 h, the restored samples were cut into beams (± 1 mm 2 adhesive interface area) and subjected to a µTBS test. Data were analyzed by two-way ANOVA test and Holm-Sidak post-hoc method (α = 0.05). The treatment with CPP-ACP pastes did not significantly affect softened dentin µTBS (p = 0.070). Statistic revealed significant reduction on µTBS values for CG/L, leading to the rejection of the second null hypothesis (p < 0.001). Both CPP-ACP based pastes did not affect µTBS of softened dentin for the adhesive system utilized. The Nd:YAG laser irradiation after application of adhesive system did affect µTBS values of softened dentin samples untreated with CPP-ACP based pastes.

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“…Therefore, root specimens without cementum were used in the present study. Demineralization of the tooth surface has been investigated through various methods, including contact microradiography 22) , polarized light microscopy 23) , nanoindentation testing 24) , confocal laser microscopy 25) , and micro-CT 26) . Among these, contact microradiography has been accepted as the standard technique for determination of mineral content and caries lesion depth.…”

Section: Discussionmentioning

confidence: 99%

<i>In vitro</i> assessment of a calcium-fluoroaluminosilicate glass-based desensitizer for the prevention of root surface demineralization

Miyajima

Ishimoto

et al. 2016

Dent. Mater. J.

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The purpose of this study was to evaluate the ability of a calcium-fluoroaluminosilicate glass-based desensitizer (Nanoseal) to protect against root demineralization in vitro. Nanoseal was applied to human root dentin, which was immersed in acidic buffer for 72 h, or exposed to pH cycling by immersing in distilled water or mineralizing solution for 24 h intermediately during 48 h-acid attack. Demineralization was evaluated by μ-CT, and mineral loss (ML) and lesion depth (LD) were determined from mineral density profiles. ML and LD in all treatment groups were significantly smaller compared with control. The Nanoseal-treated group with pH cycling using mineralizing solution had the lowest ML and LD. Analysis using an EPMA demonstrated calcium and phosphorous were incorporated into the superficial layer of specimens in the Nanoseal-treated groups, suggesting Nanoseal modified the dentin surface, making it resistant to demineralization. Application of Nanoseal is an effective method for protecting root from demineralization.

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Effects of CPP-ACP with sodium fluoride on inhibition of bovine enamel demineralization: A quantitative assessment using micro-computed tomography

Cited by 102 publications

References 47 publications

Effects of a coating resin containing S-PRG filler to prevent demineralization of root surfaces

Effects of a coating resin containing S-PRG filler to prevent demineralization of root surfaces

The effect of CPP-ACP and Nd:YAG laser on the bond strength of softened dentin

<i>In vitro</i> assessment of a calcium-fluoroaluminosilicate glass-based desensitizer for the prevention of root surface demineralization

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