Effects of creatine in a rat intestinal model of ischemia/reperfusion injury

Orsenigo, M.; Porta, Cristina; Sironi, Chiara; Laforenza, Umberto; Meyer, Gerd; Tosco, M.

doi:10.1007/s00394-011-0222-3

Cited by 11 publications

(5 citation statements)

References 43 publications

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“…There is no study which adequately demonstrates the role of the Na+/K+-ATPase pump in the development of mesenteric I/R injury. In previous in vitro studies and transplantation models; Na+/K+-ATPase pump effects have been evaluated [13,21]. Results of our study paralleled results of this study, and the Na+/K+-ATPase activity in the IR group was found to be lower than that in the control group.This decrease was found to have been significantly prevented in the IR group treated with spironolactone.In the study conducted by Federova et al, it was demonstrated that decreased Na+/K+-ATPase activity with inflammation improved following administration of aldosterone antagonist [22].…”

Section: Discussionsupporting

confidence: 84%

The Protective Effect of Spironolactone and Role of the Na /K -ATPase Pump on Intestinal Ischemia/Reperfusion Injury

Akyüz¹,

Uzun²,

Sunamak³

et al. 2018

jrp

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The aim of this study was to evaluate the possible protective effect of spironolactone (SPL) and role of the Na-K ATPase pump on intestinal ischemia/reperfusion injury. In our study, the period of ischemia was established by clamping the mesenteric artery for 45 minunder anesthesia in Wistar albino rats and the animals left for reperfusion at the end of this period were decapitated after one hour. Spironolactone (20 mg kg-1) was administered orally for three days before ischemia, 30 minbefore ischemia. The control group rats were subjected to the Sham operation and administered saline solution. TNF-α and IL-1β levels were measured in the serum samples. Ileal Na+/K+-ATPase, myeloperoxidase (MPO) analysis were performed. Structural injury was assessed histopathologically. Ischemia/reperfusion increased serum TNF-α and IL-1β levels together with MPO activity, whereas these values were maintained at the control group levels through SPL activation. However, ischemia/reperfusion decreased Na+/K+-ATPase activity in ileal tissues; however, these parameters were found to be significantly increased with SPL activation. The protective effect of SPL against ischemia/reperfusion injury by different mechanisms, mainly the activity of the Na+/K+-ATPase pump, suggests that this nontoxic agent may constitute a new clinical therapeutic principle.

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Section: Discussionsupporting

confidence: 84%

The Protective Effect of Spironolactone and Role of the Na /K -ATPase Pump on Intestinal Ischemia/Reperfusion Injury

Akyüz¹,

Uzun²,

Sunamak³

et al. 2018

jrp

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“…Previous experiments have shown that a creatine transporter operates at the brush border level of the rat jejunal enterocyte [4,38]. Moreover, in vitro treatment with creatine had positive effects on rat jejunal epithelium under conditions of oxidative stress induced by an ischemia and reperfusion model in vitro [39]. In the present study, the effect of creatine was studied in vivo, by a subministration of creatine lasting for 11 days, while oxidative stress was induced by acidosis.…”

Section: Introductionmentioning

confidence: 80%

Effects of Creatine Treatment on Jejunal Phenotypes in a Rat Model of Acidosis

et al. 2019

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We investigated the effects of creatine treatment on jejunal phenotypes in a rat model of oxidative stress induced by acidosis. In particular, the activities of some antioxidant enzymes (superoxide dismutase, glutathione peroxidase, catalase, and glutathione reductase), the level of lipid peroxidation, the expression of heat shock proteins (HSP70), and the expression of the major carriers of the cells (Na+/K+-ATPase, sodium-glucose Transporter 1—SGLT1, and glucose transporter 2—GLUT2) were measured under control and chronic acidosis conditions. Creatine did not affect the activity of antioxidant enzymes in either the control or acidosis groups, except for catalase, for which the activity was reduced in both conditions. Creatine did not change the lipid peroxidation level or HSP70 expression. Finally, creatine stimulated (Na+/K+)-ATPase expression under both control and chronic acidosis conditions. Chronic acidosis caused reductions in the expression levels of GLUT2 and SGLT1. GLUT2 reduction was abolished by creatine, while the presence of creatine did not induce any strengthening effect on the expression of SGLT1 in either the control or chronic acidosis groups. These results indicate that creatine has antioxidant properties that are realized through direct interaction of the molecule with reactive oxygen species. Moreover, the administration of creatine seems to determine a functional strengthening of the tissue, making it more resistant to acidosis.

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“…In addition, Madaeva et al demonstrated a direct relationship between oxidative stress levels and HSP70 expression in apneic patients 44 . Hypoxia also induces HSP70 expression in many organs, including the heart, liver, and intestine 45 – 49 . Compressed neck skin appears to be in more severe focal ischemia with cell damage, and HSP70 expression may be upregulated to protect the skin cells from hypoxic cell damage.…”

Section: Discussionmentioning

confidence: 99%

Forensic application of epidermal expression of HSP27 and HSP70 for the determination of wound vitality in human compressed neck skin

Zhang

Ishida

Ishigami

et al. 2023

Sci Rep

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Estimating the age and vitality of human skin wounds is essential in forensic practice, and the use of immunohistochemical parameters in this regard remains a challenge. Heat shock proteins (HSPs) are evolutionarily conserved universal proteins that protect biological systems from various types of stress. However, its importance in forensic pathology for determining wound activation in neck compression skin remains unclear. The expression of HSP27 and HSP70 in neck skin samples was immunohistochemically examined to understand its forensic applicability in determining wound vitality. Skin samples were obtained from 45 cases of neck compression (hanging, 32 cases; strangulation, 10 cases; manual strangulation, 2 cases; other, 1 case) during forensic autopsies; intact skin from the same individual was used as a control. HSP27 expression was detected in 17.4% of keratinocytes in the intact skin samples. In the compressed region, the frequency of HSP27 expression in keratinocytes was 75.8%, which was significantly higher than that in intact skin. Similarly, HSP70 expression was 24.8% in intact skin samples and 81.9% in compressed skin samples, significantly higher in compressed skin than in intact skin samples. This increase in case compression cases may be due to the cell defence role of HSPs. From a forensic pathology perspective, the immunohistochemical examination of HSP27 and HSP70 expression in neck skin could be considered a valuable marker for diagnosing traces of antemortem compression.

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Effects of creatine in a rat intestinal model of ischemia/reperfusion injury

Abstract: The presence of creatine seems to potentiate the defensive response of both tissues, in jejunum by means of cell antioxidant equipment, in ileum by the involvement of HSP70.

Cited by 11 publications

References 43 publications

The Protective Effect of Spironolactone and Role of the Na /K -ATPase Pump on Intestinal Ischemia/Reperfusion Injury

The Protective Effect of Spironolactone and Role of the Na /K -ATPase Pump on Intestinal Ischemia/Reperfusion Injury

Effects of Creatine Treatment on Jejunal Phenotypes in a Rat Model of Acidosis

Forensic application of epidermal expression of HSP27 and HSP70 for the determination of wound vitality in human compressed neck skin

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