Effects of cyclic stretch on proliferation of mesenchymal stem cells and their differentiation to smooth muscle cells

The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells

“…Furthermore, hBMMSCs align perpendicularly to the uniaxial strain axis but not that of the equiaxial strain (Park et al, 2004). In fact, the majority of studies using 2D models have been performed with uniaxial cyclic strain and result in SMC-like cells (Hamilton et al, 2004;Kurpinski et al, 2009;Jang et al, 2011;Ghazanfari et al, 2009). 3D models that are designed to replicate the status of SMCs in vivo because the radial distension of the tubular grafts that is induced by blood flow pressure allow cells to experience an effective uniaxial cyclic stain, similar to that in their natural environment (Du et al, 2011;Zheng et al, 2012).…”

Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells

“…Accumulating evidence suggests that mechanical stimuli, such as pressure [2], shear stress [3], and stretch [4], modulate MSC differentiation through mobilization of second messengers, such as inositol trisphosphate (IP 3 ) [5] and Ca 21 [6]. Spontaneous Ca 21 oscillations and stimulation induce a cytosolic Ca 21 increase, which has been well defined to regulate the expression level of transcription related genes [7,8].…”

Status of tissue engineering and regenerative medicine in Iran and related advanced tools: Bioreactors and scaffolds

“…There are many universities and government research institutes in Iran, such as Royan Institute [24][25][26], Amirkabir University [19,20,[27][28][29][30] , Pasteur Institute of Iran [31][32][33][34], University of Tehran [20,35], Tarbiat Modares University [20,[36][37][38][39], Shaheed Beheshti University of Medical Sciences [40,41,43,45], Tehran University of Medical Sciences [42,44], National Institute for Genetic Engineering [46], Islamic Azad University [47][48][49], Iran University of Science and Technology [50,51] and Iran Polymer and Petrochemical Institute [52,53] are conducting active research on the development of artificial organs based on tissue-engineering technologies.…”

“…Therefore, Ghaedi and colleagues proposed that hMSCs may serve as an effective platform for the targeted delivery of therapeutic proteins to cancer sites [60]. The other study revealed that applying appropriate loading treatment on hMSCs could enhance proliferation capability, and produce functional SMCs for engineered tissues [29]. Mohajeri and colleagues improved mechanical properties of collagen to enable mesenchymal stem cells to positively improve their proliferation and differentiation [38].…”

“…This stimulus generally applied by bioreactor to composition and ultrastructural distribution of the extracellular matrix and thus the structural integrity of TE constructs. Mechanical stimuli are particularly important for tissues that support mechanical loads in vivo, including bone, cartilage, muscle, and vasculature [16,29,[80][81][82]. Therefore, Ghazanfari and coworkers investigated influence of cyclic stretch on proliferation of hMSCs, alignment of actin filaments, and consequent differentiation to SMCs.…”