Effects of dendrobium polysaccharides on human brain microvascular endothelial cell injury induced by ox-LDL via regulating the miR-378 expression

Abstract

Brain microvascular endothelial cells are a key part of the blood-brain barrier. This experiment was set up to investigate the effect and molecular mechanism of Dendrobium polysaccharide on oxidized low-density lipoprotein (ox-LDL)-induced damage to the human brain microvascular endothelial cells.For this purpose, human brain microvascular endothelial cells HBMEC were divided into control group (without any treatment), ox-LDL group (50 μg/mL ox-LDL), Dendrobium polysaccharide low, medium and high concentration group (0.1 μg/L, 0.2 μg/L, 0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL), ox-LDL+miR-NC group (transfection miR-378 mimic negative control+50 μg/mL ox-LDL), ox-LDL+miR-378 group (transfected miR-378 mimics+50 μg/mL ox-LDL), ox-LDL+DP+anti-miR-NC group (transfected miR-378 inhibitor negative control +0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL), ox-LDL+DP+anti-miR-378 group (transfected miR-378 inhibitor+0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL ). The kit was used to detect the levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and catalase (CAT); flow cytometry to detect apoptosis; and Western blot to detect B-cell lymph tumor/leukemia-2 (Bcl-2) and Bcl-2 related X (Bax) protein expression; real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the expression of miR-378. Results showed that after treatment with different concentrations of Dendrobium polysaccharides, MDA levels were decreased in ox-LDLinduced human brain microvascular endothelial cells, SOD and CAT activities were increased, apoptosis rate was decreased, Bcl-2 expression was increased, Bax expression was decreased, miR-378 expression was increased, in a dose-dependent manner (P<0.05). Overexpression of miR-378 inhibits ox-LDL-induced oxidative stress and apoptosis in human brain microvascular endothelial cells. Inhibition of miR-378 expression reversed the effect of Dendrobium polysaccharide on ox-LDL-induced damage to human brain microvascular endothelial cells. Then dendrobium polysaccharide may inhibit ox-LDL-induced oxidative stress and apoptosis in human brain microvascular endothelial cells by up-regulating the expression of miR-378.

Dendrobium officinale polysaccharide inhibits vascular calcification via anti‐inflammatory and anti‐apoptotic effects in chronic kidney disease

“…Previous studies have shown that DOP suppresses inflammation and oxidative stress in mice with colitis 9 and ox-LDL-induced oxidative stress and apoptosis in human brain microvascular endothelial cells. 29 Given that both inflammation and apoptosis play an important role in vascular calcification, 16,17,30 it was speculated that DOP may inhibit vascular calcification. Interestingly, we found that DOP treatment inhibited the calcification of rat VSMCs.…”

Regulation of Dendrobium Polysaccharides on Proliferation and Oxidative Stress of Human Umbilical Vein Endothelial Cells in the High Glucose Environment

“…PDC is one of the main active ingredients of Dendrobium candidum and has good anti-inflammatory, antioxidant, and other biological activities [10,[21][22][23]. Different concentrations of PDC have been identified by Chen et al to incresease the activity of SOD, which could contribute to inhibiting oxidative stress and apoptosis of human brain microvascular endothelial cells [24]. The results of this experimental study found that the intracellular ROS level increased significantly, and the activity of SOD in the culture medium decreased under high glucose culture, confirming once again the role of high glucose in oxidative damage and influencing endothelial cell proliferation during incubation with endothelial cells.…”

Expression of p53, bax and bcl-2 and predictive value of fibrinogen, D-Dimer, and mean platelet volume in patients with acute cerebral infarction after intravenous thrombolysis

“…Cerebral infarction is also known as ischemic stroke, as it is called stroke in traditional Chinese medicine. It is a blood supply disorder in the local brain tissue area caused by various reasons, leading to ischemic and hypoxic necrosis of brain tissue, and then resulting in corresponding neurological deficits clinically (1)(2)(3). Cerebral infarction is classified into cerebral thrombosis, cerebral embolism, and lacunar cerebral infarction according to the different pathogeneses.…”