Effects of Development on Acetoacetyl-CoA Synthetase Biosynthesis in Rat Liver.

cDNA‐derived amino acid sequence of acetoacetyl‐CoA synthetase from rat liver1

“…AA‐CoA synthetase activity was assayed as described previously [2]. AA‐CoA synthetase protein concentration was estimated by enzyme immunoassay using anti‐rat AA‐CoA synthetase antiserum of rabbit [5] as described previously [9].…”

The lipogenic enzyme acetoacetyl-CoA synthetase and ketone body utilization for denovo lipid synthesis, a review

“…Its activity was first measured by Stern in 1971 ( 1 ) in rat liver. Its developmental pattern in brain ( 6 ) and liver ( 2 , 3 , 27 ) correlates with the development of lipogenesis in these tissues. For instance, it is high in developing brain during the period of myelination and then is much lower in adult brain when less lipid synthesis is occurring ( 2 ).…”

“…For instance, it is high in developing brain during the period of myelination and then is much lower in adult brain when less lipid synthesis is occurring ( 2 ). In rat liver, AACS activity is low in infancy until weaning and then increases to adult levels ( 2 , 3 , 27 ). In mammary gland of the female rat, the activity follows that of lipid synthesis that is needed to produce milk ( 2 ).…”

High-Fat-Diet Suppressed Ketone Body Utilization for Lipogenic Pathway in Brown Adipose Tissues

“…In ketone body utilization, the mitochondrial enzyme succinyl-CoA: 3-oxoacid CoA-transferase (SCOT, EC 2.8.3.5) is responsible for the generation of ATP from ketone bodies [8]. However, we previously identified acetoacetyl-CoA synthetase (AACS, EC 6.2.1.16) as a lipogenic ligase that directly activates acetoacetate, a ketone body in the cytosol [9,10]. AACS is highly expressed in lipogenic tissues, such as the brain, liver, and adipose tissue [10][11][12].…”

“…However, we previously identified acetoacetyl-CoA synthetase (AACS, EC 6.2.1.16) as a lipogenic ligase that directly activates acetoacetate, a ketone body in the cytosol [9,10]. AACS is highly expressed in lipogenic tissues, such as the brain, liver, and adipose tissue [10][11][12]. In the liver, AACS knockdown using the hydrodynamics method decreases serum total cholesterol levels [13].…”