Effects of Diet, Ginger Root Oil, and Elevation on the Mating Competitiveness of Male Mediterranean Fruit Flies (Diptera: Tephritidae) from a Mass-Reared, Genetic Sexing Strain in Guatemala

Shelly, Todd E.; Rendón, Pedro; Hernández, Emilio; Salgado, Sergio Zamora; McInnis, Donald O.; Villalobos, Ethel M.; Liedo, Pablo

doi:10.1603/0022-0493-96.4.1132

Cited by 19 publications

(12 citation statements)

References 24 publications

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“…However, this was evident only for the first mating. In addition, in this study the exposure to GRO did not increase the mating duration of the sterile males, and this result is in agreement with other studies (Shelly et al. 2003; Paranhos et al.…”

Section: Discussionsupporting

confidence: 94%

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Exposure of sterile Mediterranean fruit fly (Diptera: Tephritidae) males to ginger root oil reduces female remating

Morelli

Paranhos

Coelho

et al. 2010

J Applied Entomology

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Females of Ceratitis capitata are facultative polyandrous, with remating more common in laboratory strains rather than wild ones. In the application of the Sterile Insect Technique (SIT) against this pest, large overflooding ratios of sterile : wild males can increase the remating frequency. Females that mate for the first time with a sterile male tend to remate more frequently. The exposure of sterile males to ginger root oil (GRO) is used in C. capitata SIT programmes to increase the sterile male mating success. Exposing males to an ‘aromatherapy’ with GRO may also increase the remating frequency among wild females. The frequency of wild females remating, number of matings per female, the refractory period between the first and second mating, and the duration of the first and second matings of wild females were determined under laboratory conditions for three mating scenarios that included wild males only or wild males competing with sterile males (either GRO‐treated or non‐treated). Wild females first mated with sterile males exposed to GRO had their remating rate over the following 6 days and the mean number of matings per female reduced in comparison to those first mated with non‐exposed sterile males, from 62.5% to 32.2% and from 3.1 to 1.6 respectively. The remating parameters of females mated with sterile GRO‐exposed males resembled those of females mated with wild males.

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Section: Discussionsupporting

confidence: 94%

“…2003) as sterile male mating competitiveness may be increased by up to three times (Shelly and McInnis 2001; McInnis et al. 2002; Shelly et al . 2002, 2003; Paranhos et al.…”

Section: Introductionmentioning

confidence: 99%

Exposure of sterile Mediterranean fruit fly (Diptera: Tephritidae) males to ginger root oil reduces female remating

Morelli

Paranhos

Coelho

et al. 2010

J Applied Entomology

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“…Accordingly, various approaches have been examined. These include protein supplements to the pre-release diet (Maor et al, 2004;Barry et al, 2007), Juvenile hormone treatments (Teal et al, 2000), probiotic diets (Niyazi et al, 2004), and aromatherapy (Papadopoulos et al, 2001;Shelly et al, 2003). However, with the exception of aromatherapy, none have been implemented on a large scale.…”

Section: Introductionmentioning

confidence: 99%

Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance

2009

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The sterile insect technique (SIT) is a method of biological control whereby millions of factory reared sterile male insects are released into the field. This technique is commonly used to combat the Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae). Sterile medfly males are less competent in attracting and mating with wild females, a property commonly linked to the irradiation process responsible for the sterilization. As bacteria are important partners in the fly's life cycle, we used molecular analytical methods to study the community structure of the gut microbiota in irradiated male medflies. We find that the sterilizing irradiation procedure affects the gut bacterial community structure of the Mediterranean fruit fly. Although the Enterobacteriaceae family remains the dominant bacterial group present in the gut, the levels of Klebsiella species decreases significantly in the days after sterilization. In addition, we detected substantial differences in some bacterial species between the mass rearing strain Vienna 8 and the wild strain. Most notable among these are the increased levels of the potentially pathogenic species Pseudomonas in the industrial strain. Testing the hypothesis that regenerating the original microbiota community could result in enhanced competitiveness of the sterile flies, we found that the addition of the bacterial species Klebsiella oxytoca to the postirradiation diet enables colonization of these bacteria in the gut while resulting in decreased levels of the Pseudomonas sp. Feeding on diets containing bacteria significantly improved sterile male performance in copulatory tests. Further studies will determine the feasibility of bacterial amelioration in SIT operations.

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“…Constant research efforts are being made to improve SIT efficiency. These include manipulations of the olfactory environment of the sterile males prior to their release (Papadopoulos et al., 2001; Shelly et al., 2003, 2005), manipulations of the pre‐release diet (Yuval et al., 2002, 2007; Maor et al., 2004; Barry et al., 2007; Gavriel et al., 2009, 2010), juvenile hormone treatments (Teal et al., 2000, 2007; Pereira, 2005), and probiotic additives (Niyazi et al., 2004; Ben‐Yosef et al., 2008; Ben‐Ami et al., 2010; Gavriel et al., 2011).…”

Section: Introductionmentioning

confidence: 99%

Spatial patterns of sterile Mediterranean fruit fly dispersal

Gavriel

Gazit

Leach

et al. 2011

Entomologia Exp Applicata

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The success of the sterile insect technique (SIT) for the control of the Mediterranean fruit fly or medfly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), depends largely on the ability of sterile flies to spread in the target area and compete with the wild males for wild females. Our objectives in the present study were three‐fold: (1) to evaluate the dispersal ability of sterile male medflies and compare their spatial dispersion patterns with that of wild males, (2) to evaluate how different release methods affect subsequent spatial dispersal, and (3) to determine whether manipulating the pre‐release diet of sterile males affects their dispersal. To achieve these objectives, we conducted three experiments in the field where we quantified and analyzed the spatial and temporal dispersal patterns of sterile medflies and the dispersion of resident wild males. Overall, ca. 5% of the released sterile flies were recaptured 100 m from the release point, and ca. 2% were recaptured 200 m from the release point. The released flies rarely survived longer than 5–7 days. We repeatedly found that the spatial dispersion patterns of sterile males significantly correlated with those of wild males. Release methods strongly affected subsequent fly dispersal in the field as significantly more flies were recaptured following a scattered release vs. a central one. Finally, we show that enriching sterile fly pre‐release diet with protein did not affect subsequent dispersal in the field. We conclude that sterile males are able to match the dispersion patterns of wild males, an outcome that is highly important for SIT success. Large releases from central points distant from each other may leave many areas uncovered. Accordingly, scattered releases, repeated twice a week, will provide better coverage of all available aggregations sites. The spatial performance of protein‐fed males suggests that pre‐release diet amendments may be used without detriment as a sexual stimulant in SIT programs.

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Effects of Diet, Ginger Root Oil, and Elevation on the Mating Competitiveness of Male Mediterranean Fruit Flies (Diptera: Tephritidae) from a Mass-Reared, Genetic Sexing Strain in Guatemala

Cited by 19 publications

References 24 publications

Exposure of sterile Mediterranean fruit fly (Diptera: Tephritidae) males to ginger root oil reduces female remating

Exposure of sterile Mediterranean fruit fly (Diptera: Tephritidae) males to ginger root oil reduces female remating

Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance

Spatial patterns of sterile Mediterranean fruit fly dispersal

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