Effects of dietary vegetable oil on growth performance, digestive capacity, antioxidant capacity and expression of immune‐related genes in the hybrid grouper (<i>Epinephelus fuscoguttatus</i>♀ ×<i>E. lanceolatus</i>♂)

An, Wenqiang; Dong, Xiaohui; Tan, Beiping; Wu, Meihuan; Zhang, Shuang; Chi, Shuyan; Yang, Qihui; Liu, Hongyu; Yang, Yefeng

doi:10.1111/anu.13149

Cited by 12 publications

(4 citation statements)

References 80 publications

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“…The current study reported that L. crocea fed the diet with 100% LO had lower CAT activity and a higher concentration of MDA than the control group. Similar effects were found in Japanese seabass ( Lateolabrax japonicus ) ( Tan et al, 2017 ) and hybrid grouper ( Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) ( An et al, 2020 ). ALA has been confirmed to increase mitochondrial DNA methylation in the liver, which can induce oxidative stress in fish ( Liao et al, 2015 ).…”

Section: Discussionsupporting

confidence: 66%

Effects of High Levels of Dietary Linseed Oil on the Growth Performance, Antioxidant Capacity, Hepatic Lipid Metabolism, and Expression of Inflammatory Genes in Large Yellow Croaker (Larimichthys crocea)

Chen

et al. 2021

Front. Physiol.

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A growth experiment was conducted to evaluate the effects of dietary fish oil (FO) replaced by linseed oil (LO) on the growth performance, antioxidant capacity, hepatic lipid metabolism, and expression of inflammatory genes in large yellow croaker (Larimichthys crocea). Fish (initial weight: 15.88 ± 0.14 g) were fed four experimental diets with 0% (the control), 33.3%, 66.7%, and 100% of FO replaced by LO. Each diet was randomly attributed to triplicate seawater floating cages (1.0 × 1.0 × 2.0 m) with 60 fish in each cage. Results showed that the growth performance of fish fed the diet with 100% LO was markedly decreased compared with the control group (P < 0.05), while no remarkable difference was observed in the growth performance of fish fed diets within 66.7% LO (P > 0.05). The percentage of 18:3n-3 was the highest in the liver and muscle of fish fed the diet with 100% LO among the four treatments. When dietary FO was entirely replaced by LO, fish had a markedly higher total cholesterol, total triglyceride, low-density lipoprotein cholesterol content, and alanine transaminase activity in the serum than the control group (P < 0.05). The concentration of malondialdehyde was markedly higher, while the activity of catalase was markedly lower in fish fed the diet with 100% LO than the control group (P < 0.05). When dietary FO was entirely replaced by LO, hepatic lipid content, transcriptional levels of fatp1 and cd36, and CD36 protein expression were significantly higher, while transcriptional level of cpt-1 and CPT-1 protein expression were significantly lower than the control group (P < 0.05). As for the gene expression of cytokines, fish fed the diet with 100% LO had markedly higher transcriptional levels of il-1β, tnfα, and il-6 than the control group (P < 0.05). In conclusion, the substitution of 66.7% FO with LO had no significant effects on the growth performance of fish, while 100% LO decreased the growth performance and increased the inflammation and hepatic lipid content of fish. The increase of hepatic lipid content was probably due to the increased fatty acid uptake and decreased fatty acid oxidation in fish.

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Section: Discussionsupporting

confidence: 66%

Effects of High Levels of Dietary Linseed Oil on the Growth Performance, Antioxidant Capacity, Hepatic Lipid Metabolism, and Expression of Inflammatory Genes in Large Yellow Croaker (Larimichthys crocea)

Chen

et al. 2021

Front. Physiol.

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“…Similar to other cultured marine fish species, fish oil is the primary lipid source for hybrid groupers. Considering the non-sustainable reliance on fish oil to farm this species, several trials have investigated the impact of the dietary replacement of fish oil with alternatives [30][31][32][33]. However, studies related to the molecular basis of LC-PUFA biosynthesis and regulation, which could optimize the pathway to the efficient use of alternatives, are still limited in hybrid groupers.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization, Tissue Distribution and Differential Nutritional Regulation of Three n-3 LC-PUFA Biosynthesis-Related Genes in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Zheng

Wang

et al. 2022

Animals

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Elongases of very long-chain fatty acids (Elovls) and fatty acid desaturases (Fads) are crucial enzymes involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs). In this paper, we report the molecular cloning and characterization of three genes from the marine teleost Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂, and analyzed tissue distribution and their expression in response to dietary n-3 LC-PUFA levels after a 42-day feeding experiment. The elovl5, elovl8 and fads2 genes encoded 294, 263 and 445 amino acids, respectively, which exhibited all the characteristics of the Elovl and Fads family. Tissue distribution analysis revealed that elovl5, elovl8 and fads2 were widely transcribed in various tissues, with the highest level in the brain, as described in other carnivorous marine teleosts. The transcript levels of elovl5, elovl8 and fads2 in the liver were significantly affected by dietary n-3 LC-PUFA, and higher LC-PUFA levels repressed their expression. These results demonstrated, for the first time, the presence and nutritional modulation of elovl5, elovl8 and fads2 cDNA in the juvenile hybrid grouper. Further studies are needed to determine the functional characterization of these genes and explore the mechanism of these genes when regulated by dietary fatty lipid profiles in this species.

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“…The desaturation and elongation capacity varies with the fish species (Monroig et al, 2011). The effects of the incorporation of dietary VOs in aquaculture feeds have been previously worked out in many fish species, such as Psetta maxima (Regost et al, 2003), European sea bass, Dicentrarchus labrax (Mourente et al, 2005), rohu, Labeo rohita (Karanth et al, 2009), iridescent shark, Pangasius hypophthalmusturbot (Asdari et al, 2011), blunt snout bream, Megalobrama amblycephala (Li et al, 2015), gibel carp, Carassius auratus gibelio (Zhou et al, 2016), hybrid grouper, Epinephelus fuscoguttatus × E. lanceolatus (An et al, 2020), common carp, Cyprinus carpio (Ren et al, 2020), golden pompano, Trachinotus ovatus (Guo et al, 2021), turbot, Scophthalmus maximus (Xu et al, 2021), tiger puffer, Takifugu rubripes (Liao et al, 2021), and blackhead seabream, Acanthopagrus schlegelii (Zhu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Replacement of Fish Oil With Groundnut Oil for Developing Sustainable Feeds for Labeo rohita Fingerling

Siddiqua¹,

Khan²

2022

Front. Sustain. Food Syst.

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Groundnut oil (GO) is one of the most widely available vegetable oils (VOs) in India and throughout the world, with a global production of 6.12 million metric tons in the year 2020–2021. GO contains phytochemicals and antioxidants with a longer shelf life. Because of these benefits, GO can produce durable, low-cost, and sustainable aquaculture feeds. To evaluate the nutritional efficacy and possibility of replacing fish oil (FO) with GO, this experiment was carried out to test the effects of partial or total substitution of dietary FO by GO on the growth performance, carcass composition, antioxidant capacity, lysozyme activity, muscle fatty acid composition, and filet nutritional quality in Labeo rohita fingerling. Induced bred healthy rohu fingerlings (4.84 ± 0.13 g) were fed six isonitrogenous (400 g kg−1) and isolipidic (97 g kg−1) casein- and gelatin-containing purified diets, wherein the FO was gradually replaced by 0, 20, 40, 60, 80, and 100% GO. Fishes were stocked randomly in triplicate groups of 30 fish per tank and fed at 08:00, 12:00, and 16:00 h to apparent satiety for 8 weeks. The results showed that FO replacement with GO did not affect the growth but decreased the eicosapentaenoic (EPA), docosahexaenoic acid (DHA) levels, and n-3/n-6 ratio in the muscle of rohu fingerlings. The antioxidant capacity and lysozyme activity improved up to 60% replacement of FO with GO in diet and then declined (P > 0.05) upon further inclusion of GO in diets. Although the atherogenicity, thrombogenicity indices, and hypocholesterolemic/hypercholesterolemic (H/H) ratio did not change significantly among all the muscle samples, the highest filet lipid quality (FLQ) value was found in fish receiving a 100% FO diet. Further inclusion of dietary GO decreased the filet H/H ratio and FLQ value. In summary, replacing FO with GO at a higher level had negative consequences on the filet nutritional quality of rohu fingerlings. Therefore, FO can be replaced by GO in formulated feeds to a level of 60% without hampering the growth, antioxidant capacity, and lysozyme activity and to avoid degrading the nutritional quality of fish filet.

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Effects of dietary vegetable oil on growth performance, digestive capacity, antioxidant capacity and expression of immune‐related genes in the hybrid grouper (Epinephelus fuscoguttatus♀ ×E. lanceolatus♂)

Cited by 12 publications

References 80 publications

Effects of High Levels of Dietary Linseed Oil on the Growth Performance, Antioxidant Capacity, Hepatic Lipid Metabolism, and Expression of Inflammatory Genes in Large Yellow Croaker (Larimichthys crocea)

Effects of High Levels of Dietary Linseed Oil on the Growth Performance, Antioxidant Capacity, Hepatic Lipid Metabolism, and Expression of Inflammatory Genes in Large Yellow Croaker (Larimichthys crocea)

Molecular Characterization, Tissue Distribution and Differential Nutritional Regulation of Three n-3 LC-PUFA Biosynthesis-Related Genes in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Replacement of Fish Oil With Groundnut Oil for Developing Sustainable Feeds for Labeo rohita Fingerling

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