Effects of Dietary Vibroactivated Clinoptilolite Supplementation on the Intramammary Microbiological Findings in Dairy Cows

Abstract

The aim of this study was to determine the effects of dietary vibroactivated clinoptilolite supplementation on the intramammary microbiological findings in dairy cows, causative agents of potential intramammary infection, and their sensitivity to antibiotics. Cows (n = 78) were randomly divided into two groups: CPL-treated group that received clinoptilolite (CPL) in-feed (n = 38), i.e., 50 g natural powdered zeolite CPL, twice daily from the seventh month of pregnancy to 75 days after calving, and the control group (CON) of untreated cows (n = 40). Milk samples were taken from each cow on days 7, 25, 45, and 75 postpartum. The following causative pathogens were isolated in 86 udder quarters: Staphylococcus aureus in 5.81% of positive samples, Staphylococcus spp. 9.32%, coagulase-negative Staphylococcus (CNS) 22.09%, Streptococcus uberis 13.95%, Streptococcus agalactiae 1.16%, Streptococcus sp. 3.49%, Escherichia coli 8.13%, Enterococcus spp. 6.98%, Corynebacterium spp. 11.63%, Pasteurella sp. 10.47%, Serratia spp. 2.33%, and Arcanobacterium pyogenes, Citrobacter sp., Prototheca sp., and yeasts each in 1.16% of samples. Additionally, 3.87% of environmental microflora samples (n = 47) and 89.06% of udder samples (n = 1083) were bacteriologically negative. The most effective antibiotics were cefoperazone and amoxicillin-clavulanic acid, while cloxacillin and tetracycline were the least effective antibiotics in both groups. In the clinoptilolite supplemented (CPL) group (n = 38) of 14 cows, nine causative agents of mastitis were isolated in 27 quarters, while in the control (CON) group (n = 40) of 24 cows, 13 causative agents of mastitis were isolated in 59 quarters. Cows from the CON group had a 1.96 times higher risk of intramammary infection than cows from the CPL group during the observation period (odds ratio = 1.96, p = 0.0031; 95% CI = 1.2570–3.0770).

Integrated Metabolomics and Proteomics Dynamics of Serum Samples Reveals Dietary Zeolite Clinoptilolite Supplementation Restores Energy Balance in High Yielding Dairy Cows

“…NEB leads to irreversible pathophysiological damage such as future infertility if not minimized during the transition period. Previous studies have suggested that CPL dietary supplementation may improve energy status and reproductive efficiency during the postpartum period in cows [23][24][25]. The effect of long-term use of CPL have also been investigated, on different physiological statuses such as improved rumen digestion, increased glucose level, reduced subclinical ketosis, and reduced oxidative stress [26].…”

Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review

“…In another study, the impact of dietary vibroactivated and micronized clinoptilolite feeding in the incidence of intramammary infections was evaluated in dairy cows. It was observed that the antibacterial, antioxidative, immunostimulating and detoxifying potential of vibroactivated and micronized zeolite clinoptilolite reduced the incidence of intramammary infections (Đuričić et al 2020 ). Strategies have to be developed for using targeted nutrition to obtain positive host response in managing diseases.…”

Acremonium terricola Culture’s Dose–Response Effects on Lactational Performance, Antioxidant Capacity, and Ruminal Characteristics in Holstein Dairy Cows

“…Nutritional strategies have emerged, and they have been proposed as a key factor to improve the health status and welfare of animals, as well as to enhance productivity of livestock [ 2 , 3 , 4 ]. Feed additives, which are nutrients that when added to the feed can trigger the desired response of the animal’s body on production parameters, have been widely used in animal nutrition for quite a long time [ 5 , 6 , 7 , 8 ]. The main compounds used for this purpose are fermented feed, probiotics, prebiotics, synbiotics, or various types of residues from plant production.…”