Effects of different extraction techniques on physicochemical properties and activities of polysaccharides from comfrey (Symphytum officinale L.) root

Chen, Shi-lun; Shang, Hongmei; Yang, Junyan; Li, Ran; Wu, Hongxin

doi:10.1016/j.indcrop.2018.04.063

Cited by 98 publications

(43 citation statements)

References 34 publications

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“…In addition, total uronic acid content in KPS-H (45.7 ± 0.98%) was significantly ( p < 0.05) higher than that in KPS-M (43.88 ± 0.65%) and KPS-U (43.32 ± 0.73%). The results suggested that different extraction methods had significant effects on the contents of uronic acid in polysaccharides [13,14]. Indeed, the lower content of uronic acids in KPS-M and KPS-U might be attributed to the degradation of KPS under microwave and ultrasonic treatment.…”

Section: Resultsmentioning

confidence: 99%

“…Microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) have been used to extract polysaccharides from natural sources by reducing energy and time while obtaining higher bioactivities [11]. Indeed, several studies have demonstrated that natural polysaccharides extracted by MAE and UAE exhibit higher antioxidant activity than that of HWE [12,13,14,15]. However, to the best of our knowledge, the optimization of microwave-assisted extraction and ultrasonic-assisted extraction of KPS, as well as the effects of different extraction methods on their physicochemical characteristics and antioxidant activities, have seldom been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Han

Liu

et al. 2019

Molecules

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In order to evaluate effects of extraction techniques on the physicochemical characteristics and antioxidant activities of kiwifruit polysaccharides (KPS), and further explore KPS as functional food ingredients, both microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) were optimized for the extraction of KPS. Furthermore, the physicochemical structures and antioxidant activities of KPS extracted by different techniques were investigated. The optimal extraction conditions of UAE and MAE for the extraction of KPS were obtained by response surface methodology. Different extraction techniques significantly affected the contents of uronic acids, molecular weights, molar ratios of constituent monosaccharides, and the degree of esterification of KPS. Results showed that KPS exhibited remarkable DPPH and ABTS radical scavenging activities, and reducing power. The high antioxidant activities observed in KPS extracted by the MAE method (KPS-M) might be partially attributed to its low molecular weight and high content of unmethylated galacturonic acid. Results suggested that the MAE method could be a good potential technique for the extraction of KPS with high antioxidant activity, and KPS could be further explored as functional food ingredients.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Han

Liu

et al. 2019

Molecules

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“…Thus, in this study, these differences in the four polysaccharide fractions might be attributed to their different physicochemical properties, which is consistent with the result of the monosaccharide analysis. In addition, the absorption peaks at 890 and 763 cm −1 were evidence of the presence of a β-configuration and d -glucopyranose ring, respectively [ 18 ]. Overall, our results demonstrated that ASWP, ASEP-A, ASEP-C, and ASEP-P showed the typical absorption peaks of polysaccharides, having a similar backbone and chemical groups, but that sugar compositions of Aster scaber polysaccharides can be affected by different extraction methods.…”

Section: Resultsmentioning

confidence: 99%

“…Many researchers have studied the extraction, purification, structure elucidation, and pharmacological activities of plant-derived polysaccharides [ 6 , 14 , 15 , 16 , 17 ]. The extraction techniques significantly affect the yield, physicochemical properties, and biological activities of polysaccharides [ 18 ]. Research on the extraction yield of polysaccharides is garnering increased attention due to improved extraction techniques.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber

Song

Sung

Shin

et al. 2018

IJMS

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The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties. However, the biological effects of its polysaccharides are not well understood. Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods. Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively. The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP). The highest yield (3.8%) was achieved for ASEP-P extracted using pectinase digestion. Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid. Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity. ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts. The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.

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“…In our study, the results revealed that the RYRP had significant scavenging ability against the DPPH radical and ABTS + radical. According to previous research , the mechanism might be due to that the RYRP can donate electrons or hydrogen atoms to the radical cation. Iron induces the formation of free radical by catalyzing the conversion of superoxide radical to toxic hydroxyl radical .…”

Section: Discussionmentioning

confidence: 99%

Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice

Luo

Sun

et al. 2019

Biotech and App Biochem

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Red yeast rice (RYRP) has been utilized for coloring food, brewing wine, and preserving meat, which is also used as a folk medicine for centuries. In this study, a water‐soluble nonstarch polysaccharide from RYRP was extracted by using ultrasonic‐assisted extraction method. By using the Box–Behnken design to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37 ± 0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 Min), and ultrasonic power (200 W). Moreover, monosaccharide composition analysis showed that RYRP was consisted of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.152:0.015:1:0.149. The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da. Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal‐protective effect in vivo using gastrointestinal disorders model mice. RYRP could be explored as a potential source in the pharmaceutical and functional food industries.

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Effects of different extraction techniques on physicochemical properties and activities of polysaccharides from comfrey (Symphytum officinale L.) root

Cited by 98 publications

References 34 publications

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber

Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice

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