Effects of Different Gamma Radiation Doses on the Shooting and Growing of the One-eyed Scions of the Canes of Amasya Grape Variety

Ionizing radiations are a helpful technique and have improved financial potential in developing new and unique commercially important fruit tree varieties. The ‘Yaghouti’ grape cuttings were treated with 0 (control), 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 Gy gamma ray (γ) doses. The objectives of this study were to (1) investigate the effects of γ radiation on the survival rate, leaves, shoots, and root morphometric traits after γ irradiation; (2) Measurement of the 25, 50, and 75% lethal dose and 25, 50, and 75% growth reduction dose based on leaves, shoots, and root morphometric characteristics to estimate grape radiosensitivity; (3) Ultimately, determining the optimum dose of γ irradiation based biological responses (LD25, 50, 75 and GR25, 50, 75) in Yaghouti grape. The findings demonstrate that the lethal dose of the ‘Yaghouti’ was 18 Gy for LD25, 30 Gy for LD50, and 48 Gy for LD75, respectively. Furthermore, our findings showed that increasing the γ dose had a harmed ngative effect on vine growth, as evidenced by a decrease in plant height, root number, root volume, leaf area, aerial biomass, root biomass, and internode number of the ‘Yaghouti’ grape plants. Our results showed that between the aerial parts of vines, the leaf area and aerial biomass had higher radiosensitivity than plant height and other aerial parts of the plants based on data from GR25, GR50, and GR75. Moreover, GR studies of root characteristics revealed that root number and biomass root had higher radiation sensitivity than root volume. According to biological responses (LD25, 50, 75 and GR25, 50, 75) in the ‘Yaghouti’ grape, 30 Gy of γ radiation is the optimum dose for preliminary mutagenesis investigations.

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Genetic Diversity and Relationships Among Grapevine Rootstock Mutants Through Rapd Technique

Salama

Zayan

Shereif

et al. 2020

Journal of Productivity and Development

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Mutation using radiation has been shown to be an important tool in incorporating specific desirable agronomic value characteristics. In the present study, four grapevine rootstocks namely: Freedom, Harmony, SO4 and Ramsey along with commercial variety Thompson seedless were exposed to different doses of gamma rays (0, 10, 20 Gy) to obtain mutations with economic values.Results showed that both doses of 10 and 20 Gy were effective in obtaining mutations in all grapevine genotypes including Thompson seedless, which were confirmed by morphological markers such as bud burst percentage and shoot length and number of leaves, and genetic markers by RAPD technique.In addition, genetic variation and relationships in both irradiated and un-irradiated genotypes were determined. Six RAPD markers were able to generate polymorphic bands ranged from 1 to 9 among irradiated and un-irradiated grapevine genotypes. The similarity coefficients detected by RAPD markers ranged from 0.24 to 0.87, which revealed clear genetic variation among irradiated and un-irradiated grapevine genotypes. According to phylogenetic analysis, genotypes were divided into two clusters, where all five un-irradiated genotypes and irradiated Freedom genotype with 10 Gy were placed in one cluster and second cluster included the rest of nine irradiated genotypes.Conclusively, this study supports the use of mutation in breeding grapevine improvement, and RAPD technique could be an important tool for detecting mutations and genetic diversity among grapevine genotypes.

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Effects of Different Gamma Radiation Doses on the Shooting and Growing of the One-eyed Scions of the Canes of Amasya Grape Variety

Cited by 3 publications

References 2 publications

Determination of mutagenic sensitivity of hardwood cuttings of grapes ‘Red Globe’ and ‘Muscat’ ( Vitis vinifera L.) to gamma rays

Determination of mutagenic sensitivity of hardwood cuttings of grapes ‘Red Globe’ and ‘Muscat’ ( Vitis vinifera L.) to gamma rays

Determination of optimum dose based of biological responses of lethal dose (LD25, 50, 75) and growth reduction (GR25, 50, 75) in ‘Yaghouti’ grape due to gamma radiation

Genetic Diversity and Relationships Among Grapevine Rootstock Mutants Through Rapd Technique

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