Effects of dodder total flavone on polycystic ovary syndrome rat models induced by DHEA combined HCG

Abstract

Aims Explore the effects of dodder total flavone on polycystic ovary syndrome (PCOS) rat models induced by dehydroepiandrosterone (DHEA) combined human chorionic gonadotropin (HCG). Methods Except the blank group, the rest of the rats were injected with DHEA 6 mg/100 g on the back of the neck and 1.5 IU HCG each day, for 21 consecutive days. On the 16th day of modeling, vaginal smear was performed to select the model rats, which were randomly divided into model group, dacin-35 group, large, middle and small dose dodder total flavonoids groups, and given the medicine for three weeks. At the end of the last administration, take samples, so as to calculate the ovaries and uterus indexes, measure serum LH/FSH ratio, P, PRL and INS levels, fixed the uterus and pancreas in 10% formalin solution and stained with HE to observe the morphological changes of the organs. And measure the expression of TNF-α and IGF-l proteins in ovaries by immunohistochemistry. Results Compared with the blank group, ovarian and uterine indexes, serum LH/FSH ratio, serum PRL and INS levels, ovary TNF-α and IGF-l protein expression were significantly increased, and significant pathological changes were observed in the uterine and pancreatic tissues in model group ( P < 0.01). While the serum P level decreased significantly ( P < 0.01), Compared with the model group, the ovarian and uterine indexes, serum LH/FSH ratio, serum P, PRL and INS levels, ovary TNF-α protein expression were significantly decreased in large, middle and small dose dodder total flavonoids groups ( P < 0.01); The expression of IGF-1 protein was decreased and uterus pathological changes were improved in different extents ( P < 0.01 or P < 0.05), pancreas pathological changes were improved significantly ( P < 0.01). Conclusion PCOS rat models was successfully replicated. Dodder total flavone can protect PCOS rats induced by DHEA combined HCG by different action pathways.

Rhamnocitrin Ameliorates Ovarian Fibrosis Via PPARγ/TGF-β1/Smad Pathway to Repair Ovarian Function in Polycystic Ovary Syndrome Rats Running Title：rhamnocitrin Ameliorates Ovarian Fibrosis in Polycystic Ovary Syndrome Rats

“…In addition, the observations that Rhamnocitrin inhibited DHEA-induced up-regulated LH, E2, and T serum levels while promoted the downregulated FSH serum level, suggesting its bene cial role in hormonal secretion and release. Previous study reveals that total avonoids of dodder can decrease ovary index, affect serum hormone secretion and improve endometrial hyperplasia, achieving a protective effect on PCOS rats [32]. Rutin, a avonoid, ameliorates hyperandrogenism, acyclicity and infertility to improve PCOS phenotypes [10].…”

Rhamnocitrin ameliorates ovarian fibrosis via PPARγ/TGF-β1/Smad2/3 pathway to repair ovarian function in polycystic ovary syndrome rats

“…In addition, the observations that Rhamnocitrin inhibited DHEAinduced up-regulated LH, E2, and T serum levels while promoted the down-regulated FSH serum level, suggest its bene cial role in hormonal secretion and release. Previous study reveals that total avonoids of dodder can decrease ovary index, affect serum hormone secretion and improve endometrial hyperplasia, achieving a protective effect on PCOS rats [25]. Rutin, a avonoid, ameliorates hyperandrogenism, acyclicity and infertility to improve PCOS phenotypes [9].…”

Efficacy and safety of traditional Chinese medicine on treating oligomenorrhea: a systematic review and meta-analysis

“…Animal experiments have demonstrated that in addition to promoting the expression of Th1 and Th2 cytokines in T lymphocytes and enhancing the immune function of rats, the components of Radix Rehmanniae Preparata can significantly promote the proliferation of bone marrow cells, increase the number of hemoglobin and erythrocyte, and improve the recovery process of hematopoietic functions (54,55). Previous investigations have proved that chemical constituents of Semen Cuscutae can help to restore the function of hypothalamic-pituitary-gonads, promote the development of follicles, and enhance the number and function of ovarian hormone receptors (56)(57)(58)(59). Rhizoma Chuanxiong, Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, Flos Carthami, and Hirudo are all classic Chinese medicines for promoting blood circulation and removing blood stasis in China, which have been considered effective in improving blood hemorheology, anti-platelet aggregation, antioxidant, antiapoptosis, and anti-inflammatory (60)(61)(62)(63).…”