Á lvarez-Rodrı´guez, J., Palacio, J., Casasu´s, I. and Sanz, A. 2010. Does breed affect nursing and reproductive behaviour in beef cattle? Can. J. Anim. Sci. 90: 137Á143. This experiment was designed to assess the role of genetic differences in nursing behaviour and the resumption of post-partum ovarian cyclicity of beef cows with different types of calf management. Twenty-four multiparous winter-calving cows, 12 Parda de Montan˜a (PA) and 12 Pirenaica (PI), were randomly assigned to once-daily restricted nursing during 30 min (RESTR) or ad libitum nursing (ADLIB). Cow-calf behaviour was recorded at weeks 3, 8 and 13 of lactation. Results were compared within suckling system. Twice-weekly blood samples were drawn throughout lactation to analyse progesterone as an indicator of ovulation. Within each type of calf management, both breeds nursed their calves for a similar amount of time (23.0 and 57.2 min in PA vs. 25.9 and 59.0 min in PI, when nursing once daily or ad libitum, respectively; P0.10). Furthermore, ovarian cyclicity was initiated at a similar time after calving between breeds (70 vs. 73 d in PA and PI, respectively), although it was shorter in RESTR than in ADLIB (54 vs. 89 d; PB0.001).