Effects of Echinostoma caproni infection on the lipid composition of Biomphalaria glabrata

Fried, Bernard; Schafer, Susan F.; Kim, Stuart

doi:10.1016/0020-7519(89)90149-5

Cited by 25 publications

(12 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Visual observations of the chromatograms showed that triacylglycerol levels were elevated in the DGGs of H. trivolvis infected with all four larval trematodes used in this study. These results are in accord with previous observations on H. trivolvis infected with Echinostoma trivolvis and Biomphalana glabrata infected with Schistosoma mansoni (Thompson 1987), but they dier from the report study of Fried et al (1989) on E. caproni in B. glabrata and from that of McManus et al (1975) on Microphallus similis in Littorina saxatilis rudis. In the latter two studies, larval trematode infection reduced the levels of triacylglycerols in snail DGGs.…”

Section: Discussionsupporting

confidence: 81%

See 1 more Smart Citation

Thin-layer chromatography and histochemistry analyses of neutral lipids in Helisoma trivolvis infected with four species of larval trematodes

Fried

Frazer

Lee

et al. 1998

Parasitology Research

Self Cite

View full text Add to dashboard Cite

Thin-layer chromatography (TLC) and histochemistry studies were done on the neutral lipid content of the digestive gland gonad (DGG) complex of Helisoma trivolvis infected with four species of larval trematodes. Two of the species, Ribeiroia sp. and Zygocotyle lunata, contained rediae, and the two others, Spirorchis sp. and an armatae xiphidiocercaria, contained sporocysts. The DGG infected by each species had its own distinct neutral lipid profile as determined by TLC. All profiles differed from that of the uninfected DGG. Densitometric TLC studies showed some quantitative differences in free sterols in infected versus uninfected DGGs. Visual observations of the chromatograms showed that all four species caused a marked elevation in the triacylglycerol fraction in the DGG as compared with the uninfected controls. Oil Red O (ORO) histochemistry studies showed that levels of neutral lipids were increased in the DGGs of infected versus uninfected samples. These histochemistry studies showed a variable distribution of neutral fat, ranging from its absence in the cercariae of Z. lunata and the armatae xiphidiocercaria to ORO-positive droplets in the excretory system of Ribeiroia sp. Rediae and sporocysts contained ORO-positive material in the body wall and in the space between cercariae.

show abstract

Section: Discussionsupporting

confidence: 81%

“…For instance, infection of Biomphalaria glabrata with Echinostoma caproni depleted triacylglycerols in the snail digestive gland gonad (DGG) complex (Fried et al 1989), but infection of Helisoma trivolvis with E. trivolvis elevated the triacylglycerol fraction in the DGG .…”

Section: Introductionmentioning

confidence: 99%

Thin-layer chromatography and histochemistry analyses of neutral lipids in Helisoma trivolvis infected with four species of larval trematodes

Fried

Frazer

Lee

et al. 1998

Parasitology Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thompson et al (1991) analyzed neutral lipids and phospholipids in the DGGs of uninfected B. glabrata and those infected with S. mansoni and found reduced levels of phosphatidylcholine and several other phospholipids in the DGGs of snails infected with S. mansoni. Fried et al (1989) determined the effects of larval E. caproni infection on the neutral lipid content of the DGG in B. glabrata and found that the triacylglycerols, steryl esters, and free fatty acids were elevated in control DGGs compared to those in infected ones. Perez et al (1994) analyzed the phospholipids in the DGG of B. glabrata infected with E. caproni and found that the mean weight percentage of phosphatidylserine was 1.5 times greater in the DGG of infected vs uninfected snails.…”

Section: Introductionmentioning

confidence: 99%

High-performance thin-layer chromatographic analysis of neutral lipids and phospholipids in Biomphalaria glabrata patently infected with Echinostoma caproni

2006

Self Cite

View full text Add to dashboard Cite

This study examined the effects of larval trematode infection on the neutral lipid and phospholipid content of Biomphalaria glabrata patently infected with the daughter rediae of Echinostoma caproni. Uninfected snails were used as matched controls. As determined by qualitative high-performance silica gel thin-layer chromatography (HPTLC), the major neutral lipids present in the whole bodies and digestive gland-gonad complexes in both snail populations were free sterols, free fatty acids, and triacylglycerols, and the major polar lipids were phosphatidylcholine and phosphatidylethanolamine. Quantitative analysis by HPTLC with visible and UV scanning reflectance densitometry showed no significant differences in the concentrations of these lipids in whole bodies of infected snails vs the controls, but the concentration of triacylglycerols in the infected digestive gland-gonad complex was significantly less than that of the uninfected. No qualitative differences in neutral lipids and phospholipids in shell or plasma samples were found between infected vs uninfected snails.

show abstract

“…The pathobiochemical effects of larval trematodes on naturally or experimentally infected freshwater snails were considered by several authors; these effects include changes of proteins and enzymes (Loker and Hertel 1987;Nabih et al 1990;Zelck et al 1995;el-Ansary et al 2000;Mostafa et al 2001;Mostafa 2002;Mahmoud et al 2002), messenger RNA (Crews and Yoshino 1991), sugar content (Crews and Yoshino 1990;Perez et al 1994), and lipids (Fried et al 1989;Thompson 1987).…”

Section: Introductionmentioning

confidence: 99%

Effects of Schistosoma mansoni and Schistosoma haematobium infections on calcium content in their intermediate hosts

Mostafa

2007

Parasitol Res

View full text Add to dashboard Cite

Flame atomic absorption spectrometry was performed to determine the alteration of calcium concentration in the soft parts and shells of Biomphalaria alexandrina and Bulinus truncatus due to the infection with Schistosoma mansoni and S. haematobium, respectively. The results showed significant lowering in the calcium content of the shells of cercariae shedding B. alexandrina and B. truncatus relative to the calcium content in the shells of uninfected ones. In contrast, the calcium content in the soft parts of cercariae shedding snails was higher than in the soft parts of uninfected snails; the differences were statistically significant. Generally, calcium content was significantly higher in the shells than in the soft parts of the snails, regardless infected or uninfected. The results obtained and the hypothesis of hypercalcification in shells of infected snails were discussed.

show abstract

Effects of Echinostoma caproni infection on the lipid composition of Biomphalaria glabrata

Cited by 25 publications

References 5 publications

Thin-layer chromatography and histochemistry analyses of neutral lipids in Helisoma trivolvis infected with four species of larval trematodes

Thin-layer chromatography and histochemistry analyses of neutral lipids in Helisoma trivolvis infected with four species of larval trematodes

High-performance thin-layer chromatographic analysis of neutral lipids and phospholipids in Biomphalaria glabrata patently infected with Echinostoma caproni

Effects of Schistosoma mansoni and Schistosoma haematobium infections on calcium content in their intermediate hosts

Contact Info

Product

Resources

About